Relation between Gastric Cancer and Protein Oxidation, DNA Damage, and Lipid Peroxidation

Abstract

Objects. The aim of this study is to evaluate protein oxidation, DNA damage, and lipid peroxidation in patients with gastric cancer and to investigate the relationship between oxidative stress and gastric cancer. Methods. We investigated changes in serum protein carbonyl (PC), advanced oxidation protein products (AOPP), and 3-nitrotyrosine (3-NT) levels, as indicators of protein oxidation, serum 8-hydroxydeoxyguanosine (8-OHdG), as a biomarker of DNA damage, and malondialdehyde (MDA), conjugated diene (CD), 4-hydroxynonenal (4-HNE), and 8-ISO-prostaglandin F2α (8-PGF) in serum, as lipid peroxidation markers in gastric cancer (GC) patients and healthy control. Results. Compared with control, a statistically significant higher values of 8-OHdG, PC, AOPP, and 3-NT were observed in the GC patients (P < 0.05). The products of lipid peroxidation, MDA, CD, 4-HNE, and 8-PGF, were significantly lower in the GC patients compared to those of control (P < 0.05). In addition, the products of oxidative stress were similar between the Helicobacter pylori positive and the negative subgroups of GC patients. Conclusions. GC patients were characterized by increased protein oxidation and DNA damage, and decreased lipid peroxidation. Assessment of oxidative stress and augmentation of the antioxidant defense system may be important for the treatment and prevention of gastric carcinogenesis.

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“…These residues are released into the body fluid, including cerebrospinal fluid, plasma/serum, and urine under the condition of oxidative stress [1, 2]. …”

Oxidative Stress Resulting From Helicobacter pylori Infection Contributes to Gastric Carcinogenesis

“…H pylori infection results in the formation of ROS and RNS by increasing the immune cell expression of Nox and iNOS 8 . Patients infected with H pylori have increased levels of ROS along with increased levels of NO-derived metabolites, indicating the activation of iNOS 5, 18, 19, 20. In vivo studies with iNOS-deficient mice show decreased gastric cancer incidence after infection with H pylori compared with wild-type mice 21 …”

Evaluation of Immunostaining for 4-Hydroxy-2-Nonenal Receptors in Cutaneous Malignant Melanoma Immunohistochemical Study of 55 Cases

“…Moreover, it has been well documented that oxidative stress and ROS generation are intimately associated with cancer [26,27]. Accumulated data suggest that mitochondrial macromolecules derived from 4-HNE are involved in the initiation and progression of cancer [28,29].…”

“…Lipid peroxidation is initiated by the attack of free radicals on membrane lipids, generating large amounts of reactive products that have been involved in initiating and promoting cancer development [28].…”