Evaluation of Immunostaining for 4-Hydroxy-2-Nonenal Receptors in Cutaneous Malignant Melanoma Immunohistochemical Study of 55 Cases

Blendea, A; Il, Şerban; Dc, Brănişteanu; De, Brănişteanu

doi:10.4172/2155-9929.1000364

Cited by 2 publications

(1 citation statement)

References 26 publications

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“…In our study, 4HNE expression was significantly increased in CRC with a size of 2 cm or less. In earlier research, high cytoplasmic 4HNE expression was typically detected in dysplastic and early-stage malignancies, when small tumor sizes are typical (12,29). Due to the toxicity of high 4HNE accumulation, it was hypothesized that 4HNE levels decreased in later stages of cancer progression.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical expression of apolipoprotein B and 4-hydroxynonenal proteins in colorectal carcinoma patients: a retrospective study

Ng¹,

Nafi

Jalil³

et al. 2023

Croat Med J

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Aim To assess the association of the expression of apolipoprotein B (apoB) and 4-hydroxynonenal (4HNE) with the clinicopathological data of patients with colorectal cancer (CRC). Methods We obtained 80 CRC histopathological specimens sent to the Pathology Laboratory of Hospital Universiti Sains Malaysia from 2015 to 2019. Data on demographic factors, body mass index (BMI), and clinicopathological characteristics were also collected. Formalin-fixed paraffin-embedded tissues were stained by using an optimized immunohistochemical protocol. Results Patients were mostly older than 50 years, male, Malay, and overweight or obese. A high apoB expression was observed in 87.5% CRC samples (70/80), while a high 4HNE expression was observed in only 17.5% (14/80) of CRCs. The expression of apoB was significantly associated with the sigmoid and rectosigmoid tumor sites ( p =0.001) and tumor size 3-5 cm ( p =0.005). 4HNE expression was significantly associated with tumor size 3-5 cm ( p =0.045). Other variables were not significantly associated with the expression of either marker. Conclusion ApoB and 4HNE proteins may play a role in promoting CRC carcinogenesis.

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Section: Discussionmentioning

confidence: 99%

Immunohistochemical expression of apolipoprotein B and 4-hydroxynonenal proteins in colorectal carcinoma patients: a retrospective study

Ng¹,

Nafi

Jalil³

et al. 2023

Croat Med J

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The TRPA1 Channel Amplifies the Oxidative Stress Signal in Melanoma

Logu

Araújo

Ugolini

et al. 2021

Cells

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Macrophages (MΦs) and reactive oxygen species (ROS) are implicated in carcinogenesis. The oxidative stress sensor, transient receptor potential ankyrin 1 (TRPA1), activated by ROS, appears to contribute to lung and breast cancer progression. Although TRPA1 expression has been reported in melanoma cell lines, and oxidative stress has been associated with melanocytic transformation, their role in melanoma remains poorly known. Here, we localized MΦs, the final end-product of oxidative stress, 4-hydroxynonenal (4-HNE), and TRPA1 in tissue samples of human common dermal melanocytic nevi, dysplastic nevi, and thin (pT1) and thick (pT4) cutaneous melanomas. The number (amount) of intratumoral and peritumoral M2 MΦs and 4-HNE staining progressively increased with tumor severity, while TRPA1 expression was similar in all samples. Hydrogen peroxide (H2O2) evoked a TRPA1-dependent calcium response in two distinct melanoma cell lines (SK-MEL-28 and WM266-4). Furthermore, H2O2 induced a TRPA1-dependent H2O2 release that was prevented by the TRPA1 antagonist, A967079, or Trpa1 gene silencing (siRNA). ROS release from infiltrating M2 MΦs may target TRPA1-expressing melanoma cells to amplify the oxidative stress signal that affects tumor cell survival and proliferation.

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Evaluation of Immunostaining for 4-Hydroxy-2-Nonenal Receptors in Cutaneous Malignant Melanoma Immunohistochemical Study of 55 Cases

Cited by 2 publications

References 26 publications

Immunohistochemical expression of apolipoprotein B and 4-hydroxynonenal proteins in colorectal carcinoma patients: a retrospective study

Immunohistochemical expression of apolipoprotein B and 4-hydroxynonenal proteins in colorectal carcinoma patients: a retrospective study

The TRPA1 Channel Amplifies the Oxidative Stress Signal in Melanoma

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