Relation between DNA double-strand breaks and energy spectra of secondary electrons produced by different X-ray energies

Fréneau, Amélie; Santos, M. Dos; Voisin, P.; Tang, Nicolas; Vizcarra, Marta Bueno; Villagrasa, C.; Roy, Laurence; Vaurijoux, Aurélie; Gruel, Gaëtan

doi:10.1080/09553002.2018.1518612

Cited by 16 publications

(21 citation statements)

References 24 publications

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“…Errors correspond to the standard deviation (SD) of the mean over 10 batches. [30]. As can be observed, the σ rel z decreases when the dose increases.…”

Section: Yields Of Dsb/gy/gbp and Dsb Complexitymentioning

confidence: 61%

“…4(see 4.3.1.) in the irradiated cell population for the three photon energy spectra as a function of the simulated doses 0.25 Gy, 0.5 Gy, 1 Gy, and 2 Gy, as used in the experiments [30]. As can be observed, the σ decreases when the dose increases.…”

Section: Microdosimetric Calculationsmentioning

confidence: 66%

“…The experimental number of γ-H2AX foci per nucleus, determined by image analysis as shown in Figure 4, is compared to the simulated number of DSBs for chemistry stage simulation end-times of 2.5 and 10 ns in Table 3 for a dose of 1 Gy. The mean number of γ-H2AX foci per endothelial cell nucleus was observed experimentally 30 min post-irradiation for 0.25, 0.5, 1, and 2 Gy doses and obtained from at least three replicate experiments [30]. Errors correspond to the standard error.…”

Section: Comparison Between Simulated Results and Experimental Datamentioning

confidence: 99%

“…In this work, simulations were performed to reproduce experimental irradiations using different photon sources carried out at the IRSN (Institut de Radioprotection et de Sûreté Nucléaire), Fontenay aux Roses, France [30]. In Freneau et al [30], human umbilical vein endothelial cells (HUVECs) were irradiated with 40 kVp, 220 kVp, and 4 MV X-rays. The experiments showed that the yield of histone variant H2AX (γ-H2AX) foci was similar for 40 kVp and 220 kVp but higher when compared to 4 MV for a dose of 2 Gray (Gy).…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Radio-Induced Damage in Endothelial Cells Irradiated with 40 kVp, 220 kVp, and 4 MV X-rays by Means of Micro and Nanodosimetric Calculations

Tang

Bueno

Meylan³

et al. 2019

IJMS

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The objective of this work was to study the differences in terms of early biological effects that might exist between different X-rays energies by using a mechanistic approach. To this end, radiobiological experiments exposing cell monolayers to three X-ray energies were performed in order to assess the yields of early DNA damage, in particular of double-strand breaks (DSBs). The simulation of these irradiations was set in order to understand the differences in the obtained experimental results. Hence, simulated results in terms of microdosimetric spectra and early DSB induction were analyzed and compared to the experimental data. Human umbilical vein endothelial cells (HUVECs) were irradiated with 40, 220 kVp, and 4 MV X-rays. The Geant4 Monte Carlo simulation toolkit and its extension Geant4-DNA were used for the simulations. Microdosimetric calculations aiming to determine possible differences in the variability of the energy absorbed by the irradiated cell population for those photon spectra were performed on 10,000 endothelial cell nuclei representing a cell monolayer. Nanodosimetric simulations were also carried out using a computation chain that allowed the simulation of physical, physico-chemical, and chemical stages on a single realistic endothelial cell nucleus model including both heterochromatin and euchromatin. DNA damage was scored in terms of yields of prompt DSBs per Gray (Gy) and per giga (109) base pair (Gbp) and DSB complexity was derived in order to be compared to experimental data expressed as numbers of histone variant H2AX (γ-H2AX) foci per cell. The calculated microdosimetric spread in the irradiated cell population was similar when comparing between 40 and 220 kVp X-rays and higher when comparing with 4 MV X-rays. Simulated yields of induced DSB/Gy/Gbp were found to be equivalent to those for 40 and 220 kVp but larger than those for 4 MV, resulting in a relative biological effectiveness (RBE) of 1.3. Additionally, DSB complexity was similar between the considered photon spectra. Simulated results were in good agreement with experimental data obtained by IRSN (Institut de radioprotection et de sûreté nucléaire) radiobiologists. Despite differences in photon energy, few differences were observed when comparing between 40 and 220 kVp X-rays in microdosimetric and nanodosimetric calculations. Nevertheless, variations were observed when comparing between 40/220 kVp and 4 MV X-rays. Thanks to the simulation results, these variations were able to be explained by the differences in the production of secondary electrons with energies below 10 keV.

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“…Errors correspond to the standard deviation (SD) of the mean over 10 batches. [30]. As can be observed, the σ rel z decreases when the dose increases.…”

Section: Yields Of Dsb/gy/gbp and Dsb Complexitymentioning

confidence: 61%

Section: Microdosimetric Calculationsmentioning

confidence: 66%

Section: Comparison Between Simulated Results and Experimental Datamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Assessment of Radio-Induced Damage in Endothelial Cells Irradiated with 40 kVp, 220 kVp, and 4 MV X-rays by Means of Micro and Nanodosimetric Calculations

Tang

Bueno

Meylan³

et al. 2019

IJMS

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show abstract

“…Researchers have considered the impacts of low-dose radiation, given as a single dose or fractionated or prolonged over time, with regard to altering cancer progression or resolution as well as their impacts on the systemic immune system [23] .It was found that low-dose radiation increases cellular antioxidant activity; facilitates DNA damage repair; reduces malignant transformation and mutagenesis, and stimulates immune surveillance [24]. The cell nucleus and DNA are susceptible to several stressors[25, 26,27,28] and can be greatly harmed with relatively low doses of ionizing radiation [29,30]. As adverse effects of ionizing radiation on (cancer) cells are mainly mediated by fragmentation of nuclear chromatin via inserting double strand breaks (DSBs) into the DNA molecule [31].…”

Section: Discussionmentioning

confidence: 99%

Antioxidative stress of Chitosan-Gallium Nanoparticles and low doses of γ-irradiation on mice bearing Ehrlich carcinoma

Mohammad¹,

Ahmed²,

Kandil³

et al. 2019

IJASTR

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Guided treatments with nanoparticles and low doses of radiation are a new approach in cancer therapy. This study evaluated the ability of Chitosan-Gallium nanoparticles (Ch.GaNPs) and low dose ofγ-radiation to suppressoxidative stressof female mice bearing solid Ehrlich carcinoma (EC)in the neck region. Begins from 7 th day of EC cells inoculation Ch.GaNPs(0.5 mg/kg body weight) were orally administered via gavages for a period of 15 consecutive days and Whole-body γ -irradiation was carried out at a single dose of 0.25Gy/twice a week for 2 weeks. Tumor size was monitored. Oxidative stress markers were assessed, histopathological changes in tumor and liver tissues were studied. In addition, the angiogenic markers concentrations were evaluated.Treatment of EC-bearing mice with Ch.GaNPs and/or low doseof γ -radiation exposure significantly reduced tumor volume, increased lipid peroxidation and decreased glutathione content as well as glutathione peroxidase activity intumor and liver tissues. Moreover, the dual treatment significantly reducedserumVEGF, PDGF and TNF-α levels, and significantly elevated Cas-3 activity. These results were well appreciated with the histopathologicalfindings in the tumor and liver tissue. In conclusion, the combined treatment of Ch.GaNPs and low dose ofγ-radiation have a role in suppressing oxidative stress of female mice bearing solid Ehrlich carcinoma .

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Radiation-induced double-strand breaks by internal ex vivo irradiation of lymphocytes: Validation of a Monte Carlo simulation model using GATE and Geant4-DNA

Salas-Ramirez,

Maigne,

Fois

et al. 2023

Zeitschrift für Medizinische Physik

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Relation between DNA double-strand breaks and energy spectra of secondary electrons produced by different X-ray energies

Cited by 16 publications

References 24 publications

Assessment of Radio-Induced Damage in Endothelial Cells Irradiated with 40 kVp, 220 kVp, and 4 MV X-rays by Means of Micro and Nanodosimetric Calculations

Assessment of Radio-Induced Damage in Endothelial Cells Irradiated with 40 kVp, 220 kVp, and 4 MV X-rays by Means of Micro and Nanodosimetric Calculations

Antioxidative stress of Chitosan-Gallium Nanoparticles and low doses of γ-irradiation on mice bearing Ehrlich carcinoma

Radiation-induced double-strand breaks by internal ex vivo irradiation of lymphocytes: Validation of a Monte Carlo simulation model using GATE and Geant4-DNA

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