Relatedness of Group I species of Phytophthora as assessed by randomly amplified polymorphic DNA (RAPDs) and sequences of ribosomal DNA

Cooke, D. E. L.; Kennedy, D. M.; Guy, D. C.; Russell, Joanne; Unkles, Shiela E.; Duncan, James M.

doi:10.1016/s0953-7562(96)80158-4

Cited by 81 publications

(40 citation statements)

References 14 publications

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“…The ITS region of nuclear ribosomal DNA, including ITS1, ITS2 and ITS4, has been used to determine variability in fungi at the species and sub-species levels [34][35][36]. Though study on the molecular identification of Ghanaian mushrooms using the ITS region is limited, the findings in this study is in accordance with the study by Das et al [8] and Raju et al [37] who used the ITS Region to confirm the identity of Volvariella volvacea obtained from Kalyani and Tamil Nadu, different parts of India, respectively.…”

Section: Resultsmentioning

confidence: 99%

Molecular Identification of Some Ghanaian Mushrooms Using Internal Transcribed Spacer Regions

Appiah¹,

Agyare²,

Luo³

2017

Mol Biol

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Mushooms have recently attracted attention and are exploited for food and medicinal purposes. Accurate identification of mushrooms is key in utilizing them for the benefit of humans. However, morphological identification of mushrooms is time consuming, tedious and may be prone to error. DNA markers are quick and reliable tools that are useful in mushroom taxonomy. Thus this study confirmed the identity of six Ghanaian mushrooms using the internal transcribed spacer (ITS) sequences. The ribosomal DNA-ITS fragments of genomic DNA of six wild mushrooms were amplified using ITS1 and ITS4 primers. The amplicons were sequenced and data assembled and analyzed using Bio Edit. Basic Local Alignment Search Tool (BLAST) search was carried out using the National Center for Biotechnology Information (NCBI) database. The data obtained from the sequence alignment were used to plot a phylogenetic tree using the Neighbor-Joining method in Molecular Evolutionary Genetics Analysis (MEGA Phylogenetic tree showed close relationship between T. elegans and T. gibbosa, V. volvacea and P. ostreatus. Molecular identification of all six mushrooms corresponded to morphological identification up to species level. This is the first report on identification of these Ghanaian mushrooms using the ITS sequences.

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Section: Resultsmentioning

confidence: 99%

Molecular Identification of Some Ghanaian Mushrooms Using Internal Transcribed Spacer Regions

Appiah¹,

Agyare²,

Luo³

2017

Mol Biol

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“…Since then, crown rot of strawberry has been detected in most of Europe, the USA, and parts of Asia and Africa. P. cactorum can be differentiated from other Phytophthora species based on morphological characteristics, and molecular markers can distinguish between P. cactorum isolates from different host species (Cooke et al 1996, Hantula, Lilja & Parikka 1997, Lilja et al 1998. Isolates of P. cactorum from different hosts vary slightly in the morphology of the oogonia and sporangia, but not sufficiently to be used for identification (Hantula et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Genetic variation between Phytophthora cactorum isolates differing in their ability to cause crown rot in strawberry

Eikemo¹,

Klemsdal²,

Riisberg³

et al. 2004

Mycological Research

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“…This study shows that two P. cactorum and one P. citricola strains isolated from soil samples collected in the strawberry plantations where disease symptoms were observed were pathogenic both to strawberry plants and to raspberry plants, causing necrotic lesions on wounded leaf petioles and raspberry canes. The differentiation in P. cactorum isolates according to host preference was found in studies of Cooke et al [9] and Hantula et al [17]. Bielenin [3] reported that isolates of P. cactorum both from strawberry crowns and from fruit caused distinctly smaller necrosis symptoms on apple shoots than isolates from apple trees.…”

Section: Association Of Phytophthora Spp Presence With Yield Lossmentioning

confidence: 90%

Identification of Phytophthora spp. isolated from plants and soil samples on strawberry plantations in Poland

Meszka

Michalecka

2016

J Plant Dis Prot

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Crown and leather rot of strawberry caused by Phytophthora spp. are major soil diseases of cultivated strawberry (Fragaria 9 ananassa) in Poland. In this study, in total 45 isolates of Phytophthora spp. from plants of cultivars Elsanta, Honeoye, Florence, Camarosa, Roxana, Onebor, Alba, Elegance, Albion, Senga Sengana and Malwina originating from crowns (20 isolates) and fruits (25 isolates) as well as 25 soil samples from the same fruiting strawberry plantations located in central, northeastern, and south-eastern regions of Poland were identified. Among them, 44 isolates from plant organs and eight from soil samples were identified as Phytophthora cactorum and from one plant and one soil sample as P. citricola; of those remaining from the soil, one was identified as P. citrophthora and one as P. cryptogea. Identifications were based on morphological characteristics and DNA analyses. Species-specific polymerase chain reaction confirmed the presence of Phytophthora spp. in all positive examined plant and soil samples and confirmed that P. cactorum is predominantly responsible for crown and leather rot diseases of strawberry in monitored regions of Poland. Pathogenicity tests showed that all isolates of Phytophthora spp. originating from soil derived from strawberry plantations were pathogenic both to strawberry and to raspberry.

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Relatedness of Group I species of Phytophthora as assessed by randomly amplified polymorphic DNA (RAPDs) and sequences of ribosomal DNA

Cited by 81 publications

References 14 publications

Molecular Identification of Some Ghanaian Mushrooms Using Internal Transcribed Spacer Regions

Molecular Identification of Some Ghanaian Mushrooms Using Internal Transcribed Spacer Regions

Genetic variation between Phytophthora cactorum isolates differing in their ability to cause crown rot in strawberry

Identification of Phytophthora spp. isolated from plants and soil samples on strawberry plantations in Poland

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