Relapsing-Remitting Multiple Sclerosis diagnosis from cerebrospinal fluids via Fourier transform infrared spectroscopy coupled with multivariate analysis

Abstract

Multiple sclerosis (MS) is a chronic, progressive, inflammatory and degenerative disease of central nervous system. Here, we aimed to develop a method for differential diagnosis of Relapsing-Remitting MS (RRMS) and clinically isolated syndrome (CIS) patients, as well as to identify CIS patients who will progress to RRMS, from cerebrospinal fluid (CSF) by infrared (IR) spectroscopy and multivariate analysis. Spectral analyses demonstrated significant differences in the molecular contents, especially in the lipids and Z conformation of DNA of CSF from CIS, CIS to RRMS transformed (TCIS) and RRMS groups. These changes enables the discrimination of diseased groups and controls (individuals with no neurological disease) from each other using hierarchical cluster and principal component analysis. Some CIS samples were consistently clustered in RRMS class, which may indicate that these CIS patients potentially will transform to RRMS over time. Z-DNA band at 795 cm−1 that is existent only in diseased groups and significant increase in carbonyl amount, decrease in amideI/amide II and lipid/protein ratios observed only for RRMS groups can be used as diagnostic biomarkers. The results of the present study shed light on the early diagnosis of RRMS by IR spectroscopy complemented with multivariate analysis tools.

Diagnosis of malignant pleural mesothelioma from pleural fluid by Fourier transform-infrared spectroscopy coupled with chemometrics

“…ATR-FTIR spectroscopy has been proposed as an efficient useful diagnostic tool for a variety of diseases within the biomedical field toward translational medicine. 37,38 In order to visually demonstrate the spectral differences between studied groups, the average spectra of MPM, LC, and BPE pleural fluids are shown in Fig. 1, in the 3800 to 800 cm −1 spectral region.…”

4-Nonylphenol effects on rat testis and sertoli cells determined by spectrochemical techniques coupled with chemometric analysis

“…Significant alterations are observed in the lipid-to-protein ratio for the NP-treated samples (Figure 3A and 7A), indicating an alteration in the cellular lipid and protein metabolism caused by NP exposure (Yonar et al, 2018). These biomolecular alterations, in turn, may be related to NP-induced apoptosis or dysfunction of SCs and germ cells that lead to seminiferous tubule degeneration with impaired spermatogenesis.…”

Characterization and Differentiation of Adipose Tissue by Spectroscopic and Spectral Imaging Techniques

“…These alterations can be detected rapidly and sensitively without using external agents by attenuated total reflectance-fourier transform infrared (ATR-FTIR) spectroscopy [49,50]. Sen et al [51] aimed to investigate the effects of obesity on macromolecular alterations in order to characterize berlin fat mice (BFMI) lines according to the macromolecular alterations within SCAT and VAT by using ATR-FTIR spectroscopy.…”