Rejuvenation treatment of stored red blood cells reverses storage‐induced adhesion to vascular endothelial cells

Koshkaryev, Alexander; Zelig, Orly; Manny, Noga; Yedgar, Saul; Barshtein, Gregory

doi:10.1111/j.1537-2995.2009.02251.x

Cited by 57 publications

(57 citation statements)

References 55 publications

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“…Table 3 summarizes the effectiveness of the rejuvenation treatment (described by REI) in improving storage-induced damage to RBC mechanical properties (determined in the present study), as well as other RBC properties determined in our previous study [17]. This table clearly shows that treatment of St-RBC with Rejuvesol rectified all tested properties of St-RBC, but the extent of improvement differed considerably between the different measures.…”

Section: Resultsmentioning

confidence: 63%

“…Similar to our previous [17,18] as well as other studies [24,26,27], we used non-leukoreduced St-RBC. Blood was drawn from 9 healthy donors at the Hadassah Hospital Blood Bank, following informed consent according to the Helsinki Committee Regulations Permit (98290, Hadassah Hospital, Jerusalem, Israel), and collected (non-leukoreduced) into standard sterile bags (Fresenius Kabi AG, Homburg, Germany), containing citrate phosphate dextrose (CPD).…”

Section: Methodsmentioning

confidence: 99%

“…On the other hand, the rejuvenation treatment exerted practically a complete reduction of the storage-induced elevation of RBC adhesion to vascular endothelial cells (EC) [17]. These finding suggest that rejuvenation might differentially affect storage-induced changes in RBC properties.…”

Section: Introductionmentioning

confidence: 99%

“…In a previous study [17], we found that post-storage rejuvenation (with Rejuvesol) of un-leukoreduced St-RBC (stored in CPDA-1) only partially rectified storage-induced biochemical lesions, including elevated levels of reactive oxygen species (ROS) and intracellular Ca 2+ as well as phosphatidylserine (PS) externalization [17]. On the other hand, the rejuvenation treatment exerted practically a complete reduction of the storage-induced elevation of RBC adhesion to vascular endothelial cells (EC) [17].…”

Section: Introductionmentioning

confidence: 99%

“…CPDA-1, AS, SAGM, PAGGSM, CP2D, AS3). These are likely to differentially affect St-RBC properties, as well as the extent of storage-induced damage and its kinetics [5,17,18,19,20,21]. Pre-storage leukoreduction has been shown to attenuate storage-induced damage to RBC properties, including deformability [5].…”

Section: Introductionmentioning

confidence: 99%

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Storage-Induced Damage to Red Blood Cell Mechanical Properties Can Be Only Partially Reversed by Rejuvenation

Barshtein

Gural²,

Manny³

et al. 2014

Transfus Med Hemother

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Background: The storage of red blood cells (RBC) is associated with impairment of their properties that can induce a circulatory risk to recipients. In a preceding study (2009), we reported that post-storage rejuvenation (RJ) of stored RBC (St-RBC) efficiently reduced the storage-induced RBC/endothelial cell interaction, while only partially reversing the level of intracellular Ca²⁺, reactive oxygen species, and surface phosphatidylserine. In the present study, we examined the RJ effectiveness in repairing St-RBC mechanical properties. Methods: RBC, stored in CPDA-1 without pre-storage leukoreduction, were subjected to post-storage RJ, and the deformability, osmotic fragility (OF), and mechanical fragility (MF) of the rejuvenated St-RBC (St-RBC_Rj) were compared to those of untreated St-RBC and of freshly-collected RBC (F-RBC). Results: 5-week storage considerably increased OF and MF, and reduced the deformability of St-RBC. All alterations were only partially (40-70%) reversed by RJ, depending on the extent of the damage: the greater the damage, the lesser the relative effect of RJ. Conclusion: The findings of the present and preceding studies suggest that different St-RBC properties are differentially reversed by RJ, implying that some of the changes occur during storage and are irreversible.

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Section: Resultsmentioning

confidence: 63%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Storage-Induced Damage to Red Blood Cell Mechanical Properties Can Be Only Partially Reversed by Rejuvenation

Barshtein

Gural²,

Manny³

et al. 2014

Transfus Med Hemother

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The Red Blood Cell Storage Lesion: A Controversy of Biology Versus Randomized Controlled Trials

Smajdor

Spiess

2021

Essentials of Blood Product Management in Anesthesia Practice

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Vitamin E Induces Phosphatidylserine Externalization and Red Cell Adhesion to Endothelial Cells

Koshkaryev¹,

Barshtein²,

Yedgar³

2009

Cell Biochem Biophys

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Red blood cell (RBC) adhesion to vessel wall endothelium is a potent catalyst of vascular occlusion and occurs in oxidative stress states such as hemoglobinopathies and cardiovascular conditions. These are often treated with vitamin E (VitE), a "classic" antioxidant. In this study, we examined the effects of VitE on RBC adhesion to vascular endothelial cells (EC), and on translocation of phosphatidylserine (PS) to RBC surface, known as a potent mediator of RBC/EC adhesion, facilitating thrombus formation. Treatment of RBC with VitE strongly induces (up to sevenfold) PS externalization and enhances (up to 20-fold) their adherence to EC. The VitE hydrophilic analogue-Trolox-does not incorporate into cell membranes. Trolox did not exhibit any of these effects, implying that the VitE effect is due to its known ability to incorporate into cell membranes. The membrane-incorporated VitE significantly reduced the level of reactive oxygen species in H(2)O(2)-treated RBC, demonstrating that VitE elevates RBC/EC adhesion despite acting as an anti-oxidant. This study demonstrates for the first time that contrary to the common view of VitE as a beneficial supplement, VitE may introduce a circulatory risk by inducing flow-disturbing RBC adherence to blood vessel wall and the pro-thrombotic PS exposure.

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Rejuvenation treatment of stored red blood cells reverses storage‐induced adhesion to vascular endothelial cells

Abstract: Poststorage RBC rejuvenation treatment is effective in reversing the storage-induced RBC/EC interaction. This provides further documentation for the potential clinical benefit of poststorage rejuvenation.

Cited by 57 publications

References 55 publications

Storage-Induced Damage to Red Blood Cell Mechanical Properties Can Be Only Partially Reversed by Rejuvenation

Storage-Induced Damage to Red Blood Cell Mechanical Properties Can Be Only Partially Reversed by Rejuvenation

The Red Blood Cell Storage Lesion: A Controversy of Biology Versus Randomized Controlled Trials

Vitamin E Induces Phosphatidylserine Externalization and Red Cell Adhesion to Endothelial Cells

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