Rejuvenation improves roller pump–induced physical stress resistance of fresh and stored red blood cells

Gelderman, Monique P.; Vostal, Jaroslav G.

doi:10.1111/j.1537-2995.2010.02972.x

Cited by 15 publications

(19 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…treatment mainly by Rejuvesol, can completely reverse the storage-induced reduction of St-RBC levels of biochemical measures, specifically ATP and 2,3-DPG, and of mechanical properties, specifically OF, MF, and deformability [34,40,41,42]. However, as shown in table 3, in the present and previous [17] studies we found that all these properties are only partially rectified by treatment with Rejuvesol.…”

Section: Discussioncontrasting

confidence: 55%

“…While Gelderman and Vostal [41] reported complete reversal of St-RBC OF and MF, we found only partial improvement of these alterations (fig. 1 and 2, table 3).…”

Section: Discussioncontrasting

confidence: 48%

“…Specifically, it has been reported [34] that with any storage duration the increase in fragility of St-RBC was significantly higher when stored in SAGM [34] or PAGGSM [43] compared to AS-5 medium. In the study of Gelderman and Vostal [41], RBC were stored in CP2D/AS3 medium [41], while in the present study RBC were stored in CPDA-1. In a study comparing RBC storage media, Heaton et al [22] found that following 35 days of storage in CP2D/AS3, ATP levels were approximately 2-fold higher than after storage in CPDA-1 (3.22 ± 0.58 vs. 1.71 ± 0.45 µ mol/g Hb).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Storage-Induced Damage to Red Blood Cell Mechanical Properties Can Be Only Partially Reversed by Rejuvenation

Barshtein

Gural²,

Manny³

et al. 2014

Transfus Med Hemother

View full text Add to dashboard Cite

Background: The storage of red blood cells (RBC) is associated with impairment of their properties that can induce a circulatory risk to recipients. In a preceding study (2009), we reported that post-storage rejuvenation (RJ) of stored RBC (St-RBC) efficiently reduced the storage-induced RBC/endothelial cell interaction, while only partially reversing the level of intracellular Ca²⁺, reactive oxygen species, and surface phosphatidylserine. In the present study, we examined the RJ effectiveness in repairing St-RBC mechanical properties. Methods: RBC, stored in CPDA-1 without pre-storage leukoreduction, were subjected to post-storage RJ, and the deformability, osmotic fragility (OF), and mechanical fragility (MF) of the rejuvenated St-RBC (St-RBC_Rj) were compared to those of untreated St-RBC and of freshly-collected RBC (F-RBC). Results: 5-week storage considerably increased OF and MF, and reduced the deformability of St-RBC. All alterations were only partially (40-70%) reversed by RJ, depending on the extent of the damage: the greater the damage, the lesser the relative effect of RJ. Conclusion: The findings of the present and preceding studies suggest that different St-RBC properties are differentially reversed by RJ, implying that some of the changes occur during storage and are irreversible.

show abstract

Section: Discussioncontrasting

confidence: 55%

“…While Gelderman and Vostal [41] reported complete reversal of St-RBC OF and MF, we found only partial improvement of these alterations (fig. 1 and 2, table 3).…”

Section: Discussioncontrasting

confidence: 48%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Storage-Induced Damage to Red Blood Cell Mechanical Properties Can Be Only Partially Reversed by Rejuvenation

Barshtein

Gural²,

Manny³

et al. 2014

Transfus Med Hemother

View full text Add to dashboard Cite

show abstract

“…Briefly, 2 x10 7 cells were added to PBS solutions from 0% to 0.9% saline and incubated for 30 minutes as previously described [12]. After centrifugation, hemoglobin release, which directly corresponds to the number of lysed cells, was assessed by colorimetric assay by determining the absorbance of the supernatants at 540 nm using the Biotek Synergy-4 plate reader.…”

Section: Methodsmentioning

confidence: 99%

“…Cells were washed twice and analyzed using a FACSCalibur (Becton Dickinson, San Jose, CA) equipped with CellQuestPro software. To determine recovery of transfused RBCs, mouse whole blood samples containing either CFSE-labeled control RBCs or stemRBCs were washed twice and resuspended in 500 μL PBS/0.1% BSA for flow cytometric analysis [12]. …”

Section: Methodsmentioning

confidence: 99%

Evaluation of Stem Cell-Derived Red Blood Cells as a Transfusion Product Using a Novel Animal Model

et al. 2016

Self Cite

View full text Add to dashboard Cite

Reliance on volunteer blood donors can lead to transfusion product shortages, and current liquid storage of red blood cells (RBCs) is associated with biochemical changes over time, known as ‘the storage lesion’. Thus, there is a need for alternative sources of transfusable RBCs to supplement conventional blood donations. Extracorporeal production of stem cell-derived RBCs (stemRBCs) is a potential and yet untapped source of fresh, transfusable RBCs. A number of groups have attempted RBC differentiation from CD34+ cells. However, it is still unclear whether these stemRBCs could eventually be effective substitutes for traditional RBCs due to potential differences in oxygen carrying capacity, viability, deformability, and other critical parameters. We have generated ex vivo stemRBCs from primary human cord blood CD34+ cells and compared them to donor-derived RBCs based on a number of in vitro parameters. In vivo, we assessed stemRBC circulation kinetics in an animal model of transfusion and oxygen delivery in a mouse model of exercise performance. Our novel, chronically anemic, SCID mouse model can evaluate the potential of stemRBCs to deliver oxygen to tissues (muscle) under resting and exercise-induced hypoxic conditions. Based on our data, stem cell-derived RBCs have a similar biochemical profile compared to donor-derived RBCs. While certain key differences remain between donor-derived RBCs and stemRBCs, the ability of stemRBCs to deliver oxygen in a living organism provides support for further development as a transfusion product.

show abstract

Should modulation of p50 be a therapeutic target in the critically ill?

Srinivasan

Morkane

Martin

et al. 2017

Expert Review of Hematology

View full text Add to dashboard Cite

Rejuvenation improves roller pump–induced physical stress resistance of fresh and stored red blood cells

Abstract: Our studies suggest that rejuvenation improves roller pump-induced physical and osmotic stress resistance of stored RBCs.

Cited by 15 publications

References 26 publications

Storage-Induced Damage to Red Blood Cell Mechanical Properties Can Be Only Partially Reversed by Rejuvenation

Storage-Induced Damage to Red Blood Cell Mechanical Properties Can Be Only Partially Reversed by Rejuvenation

Evaluation of Stem Cell-Derived Red Blood Cells as a Transfusion Product Using a Novel Animal Model

Should modulation of p50 be a therapeutic target in the critically ill?

Contact Info

Product

Resources

About