Rejoining of cut wounds by engineered gelatin–keratin glue

Raja, S.; Thiruselvi, T.; Sailakshmi, G.; Ganesh, S.; Gnanamani, A.

doi:10.1016/j.bbagen.2013.04.009

Cited by 31 publications

(21 citation statements)

References 40 publications

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“…However, this material lost most adhesion ability if it was pre‐wetted, had no mechanism for tuneable or extended drug delivery, and lacked a biodegradable pathway. Other biodegradable adhesives exist, usually in the form of protein hydrogels, but their mechanical properties limits their application and they typically have low shear bioadhesion strengths of 50 mN cm −2 or less . A notable exception are the cyanoacrylate adhesives which displays strong adhesion force of 5000 mN cm −2 after initial compression of 5 N for 15 min and 4830 ± 1240 mN cm −2 on dry aorta tissues .…”

Section: Resultsmentioning

confidence: 99%

Novel On‐Demand Bioadhesion to Soft Tissue in Wet Environments

Mogal

Papper

Chaurasia

et al. 2013

Macromolecular Bioscience

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Current methods of tissue fixation rely on mechanical-related technologies developed from the clothing and carpentry industries. Herein, a novel bioadhesive method that allows tuneable adhesion and is also applicable to biodegradable polyester substrates is described. Diazirine is the key functional group that allows strong soft tissue crosslinking and on-demand adhesion based on a free radical mechanism. Plasma post-irradiation grafting makes it possible to graft diazirine onto PLGA substrates. When the diazirine-PLGA films, placed on wetted ex vivo swine aortas, are activated with low intensity UV light, lap shear strength of up to 450 ± 50 mN cm(-2) is observed, which is one order of magnitude higher than hydrogel bioadhesives placed on similar soft tissues. The diazirine-modified PLGA thin films could be added on top of previously developed technologies for minimally invasive surgeries. The present work is focused on the chemistry, grafting, and lap shear strength of the alkyl diazirine-modified PLGA bioadhesive films.

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Section: Resultsmentioning

confidence: 99%

Novel On‐Demand Bioadhesion to Soft Tissue in Wet Environments

Mogal

Papper

Chaurasia

et al. 2013

Macromolecular Bioscience

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“…In the case of first environment, the stability assessment was made in the presence of Phosphate buffered saline and the second environment consists of proteolytic enzyme (pepsin). At scheduled time intervals (3, 6, 9, 12 and 24 h) the degraded samples were subjected to TNBS assay 38 and SDS-PAGE analysis 39 .…”

Section: Methodsmentioning

confidence: 99%

“…Hemo-compatibility of the material was studied by both direct and indirect contact assays 39 40 using a representative sample. In the indirect method, hydrogel (450 μM) samples (10 mm dia and 10 mm height) were sterilized by immersing in 70% alcohol and rinsed with sterile PBS and then incubated for the period of 5 to10 days in PBS.…”

Section: Methodsmentioning

confidence: 99%

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pH and redox sensitive albumin hydrogel: A self-derived biomaterial

Raja

Thiruselvi

Mandal

et al. 2015

Sci Rep

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Serum albumin can be transformed to a stimuli (pH and redox) responsive hydrogel using the reduction process followed by oxidative refolding. The preparation of albumin hydrogel involves a range of concentrations (75, 150, 300, 450, 600 and 750 μM) and pH (2.0–10.0) values and the gelation begins at a concentration of 150 μM and 4.5–8.0 pH value. The hydrogel shows maximum swelling at alkali pH (pH > 9.0). The increase in albumin concentration increases hydrogel stability, rheological property, compressive strength, proteolytic resistance and rate of in vivo biodegradation. Based on the observed physical and biological properties of albumin hydrogel, 450 μM was determined to be an optimum concentration for further experiments. In addition, the hemo- and cytocompatibility analyses revealed the biocompatibility nature of albumin hydrogel. The experiments on in vitro drug (Tetracycline) delivery were carried out under non reducing and reducing conditions that resulted in the sustained and fast release of the drug, respectively. The methodology used in the preparation of albumin hydrogel may lead to the development of autogenic tissue constructs. In addition, the methodology can have various applications in tissue engineering and drug delivery.

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“…Besides asepsis and conventional therapeutics with antibiotics and anti-inflammatories, some techniques have been developed to assist in the skin repair process, such as the use of skin flaps, autografts and extracellular matrix (BILLINGHAM; MEDAWAR, 1951;BARRANDON;GREEN, 1988). Currently, novel techniques are being applied, such as negative-pressure wound therapy in birds (KNAPP-HOCH; MATOS, 2014), acupuncture (BLAIR, 2013) and gelatin-keratin glue in humans (RAJA et al, 2013). Holistic methods have also been administered in the treatment of injuries not only in birds, but also small animals (PESCH, 2014) and zoo animals, such as acupuncture, moxibustion and acupressure, which are based on stimulating particular points situated at meridians that are able to achieve responses from organs and systems of the body, especially moxibustion, which increases healing with thermogenic effects (XIE; ECKERMANN-ROSS, 2012;HUANG et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Use of a porous cellulose membrane (Membracel®) and moxibustion for severe excoriative injury in a chilean skua (Catharacta chilensis)

Mannina

Mello

Costa

et al. 2018

Braz. J. Vet. Res. Anim. Sci.

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The use of alternative therapeutic approaches in wild animals has gained notoriety due to its efficiency, low cost and reduced or nonexistent stressors. The present study evaluated the efficacy of treatment of a bilateral excoriative lesion in the region of the dorsal metacarpus in a Chilean skua (Catharacta chilensis) in a rehabilitation process with the use of a porous cell membrane, sold commercially, in concomitance with moxibustion sessions with herbs (Artemisia vulgaris). The healing time of 14 days was reduced when compared to traditional techniques and proved the viability and efficacy of the treatment. Keywords: Artemisia vulgaris. Healing. Rehabilitation. Metacarpus. Marine bird. ResumoO uso de abordagens terapêuticas alternativas em animais selvagens tem ganhado notoriedade em virtude de sua eficiência, baixo custo e estímulos estressores reduzidos ou inexistentes. Este estudo avaliou a eficácia do tratamento de uma lesão escoriativa bilateral em região do dorsal metacarpo em um mandrião chileno (Catharacta chilensis) em processo de reabilitação com o uso de uma membrana celular porosa vendida comercialmente, em concomitância com sessões de moxabustão com ervas (Artemisia vulgaris). O tempo de cicatrização de catorze dias mostrou-se reduzido quando comparado ao de outras técnicas tradicionais e provou a viabilidade e eficácia do tratamento. Palavras-chave: Artemisia vulgaris. Cicatrização. Reabilitação. Metacarpos. Ave marinha.

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Rejoining of cut wounds by engineered gelatin–keratin glue

Cited by 31 publications

References 40 publications

Novel On‐Demand Bioadhesion to Soft Tissue in Wet Environments

Novel On‐Demand Bioadhesion to Soft Tissue in Wet Environments

pH and redox sensitive albumin hydrogel: A self-derived biomaterial

Use of a porous cellulose membrane (Membracel®) and moxibustion for severe excoriative injury in a chilean skua (Catharacta chilensis)

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