Reinforced Hybrid Collagen Sponges for Tissue Engineering

Abstract

We created an anisotropic material based on collagen sponge and reactive polylactide structured by laser photopolymerization. The combination of collagen with reactive polylactide improves the resistance of the formed matrices to biodegradation in comparison with collagen sponge, while the existence of sites with different mechanical characteristics and cell affinity on the matrix provides directed cell growth during their culturing. It was shown that reinforcement of the collagen sponges 7-fold increased the mean Young's modulus for the hybrid matrix without affecting its cytotoxicity. The developed matrix provides cell adhesion and proliferation along reinforcement lines and can be used for fabrication of tissue engineering constructs.

Crosslinking of Polylactide by High Energy Irradiation and Photo-Curing

“…Functionalized with (meth)acrylate groups star low molecular weight polylactides have been used for stereolithography also by other groups where two-photon polymerization (2PP) technique was applied for crosslinking [ 70 , 71 , 72 , 73 , 74 , 75 ]. Star-shaped methacrylate-terminated oligo( d , l -lactide)s with M n = 2800 were prepared, and it was demonstrated that oligomer synthesis and their functionalization can be carried out in the same reactor [ 71 ].…”

“…Similarly, fabricated scaffolds (2PP technique) were also used for supporting of Schwann cells growth and thus, as neural scaffolds in nerve repair [ 70 ]. Laser-induced crosslinked star-shaped methacrylate-terminated oligo( d , l -lactide)s (M n = 2400) were used as a reinforcement of collagen materials [ 74 , 75 ]. The material exhibited improved resistance to biodegradation, while the direct multipotent stromal cell growth during their culture was observed.…”

“…Some authors applied a two-photon polymerization technique (2PP) as the type of stereolithography [ 109 ] for the preparation of UV crosslinked PLA-based materials designed for tissue scaffolds [ 70 , 71 , 73 , 74 , 75 ]. 2PP is a computer-aided microfabrication method by which it is possible to produce biomimetic synthetic scaffolds with high precision and reproducibility.…”

Chitosan-g-oligo/polylactide copolymer non-woven fibrous mats containing protein: from solid-state synthesis to electrospinning

“…Collagen (type I) was obtained from cattle dermis as reported earlier. 38,39 All solvents were purchased from Acros Organics (Belgium) as analytical grade and were used without further purication.…”

A Collagen Basketweave from the Giant Squid Mantle as a Robust Scaffold for Tissue Engineering

“…The sample was ≈100-fold concentrated by ultrafiltration on a Microcon Centrifugal filter unit with a 10 kDa molecular cut-off (MRCPRT010, Millipore, Burlington, MA, USA) to obtain the final collagen at 10 mg/mL. Collagen from GSCM and Type I collagen from the cattle dermis were isolated using a protocol described in [82], while Type II collagen was isolated from the tracheal cartilage by a protocol described in [83] omitting the use of pepsin. An amount of 10 µg of the proteins were diluted with an SDS-loading buffer supplemented with 100 mM DTT (20710, SERVA, Heidelberg, Germany) and heated at 95 • C for 5 min.…”