Regulatory T cells and intestinal homeostasis

Coombes, Janine L.; Robinson, Nicholas J.; Maloy, Kevin J.; Uhlig, Holm H.; Powrie, Fiona

doi:10.1111/j.0105-2896.2005.00250.x

Cited by 253 publications

(199 citation statements)

References 84 publications

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“…Peripheral blood was obtained from patients with various subtypes of CLE (SCLE, DLE, LET) (n ϭ 12) as well as from normal healthy donors (n ϭ 18) and separated by Ficoll gradient centrifugation. The detection of Treg by analysis of CD4ϩ,CD25 high cells has produced conflicting results in patients with SLE (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)31,32). Therefore, we used a new intracellular staining method to directly visualize Foxp3ϩ Treg by flow cytometry, which was performed according to the manufacturer's protocol, as described previously (25).…”

Section: Methodsmentioning

confidence: 99%

“…Treg can suppress conventional T cells (Tcon) by direct cell contact in vitro (8), but down-modulation of dendritic cell (DC) function and secretion of inhibitory cytokines, such as interleukin-10 (IL-10) and transforming growth factor ␤ (TGF␤), might contribute to Treg function in vivo (10,11). A major unanswered question about Treg-mediated suppression concerns the location at which tolerance induction occurs in vivo.…”

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confidence: 99%

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Low number of regulatory T cells in skin lesions of patients with cutaneous lupus erythematosus

Franz¹,

Fritzsching²,

Riehl³

et al. 2007

Arthritis & Rheumatism

123

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Objective. To define the phenotype and function of CD4؉,CD25؉ regulatory T cells (Treg) in patients with cutaneous lupus erythematosus (CLE), a heterogeneous autoimmune disease characterized primarily by inflammatory skin lesions.Methods. The number of Treg in skin specimens obtained from patients with various subtypes of CLE was investigated by immunohistochemical analysis, using anti-Foxp3 and anti-CD4 monoclonal antibodies. Furthermore, characterization of peripheral blood CD4؉,CD25؉ Treg from normal healthy donors and patients with CLE was carried out by flow cytometry, analyzing the expression of Foxp3 and Treg subpopulations. We also purified CD4؉,CD25 high Treg obtained from patients with CLE and tested the sensitivity of these cells to CD95L-mediated apoptosis.Results. Quantitative analysis of CD4؉ T cells in skin lesions from patients with CLE revealed that the number was similar to that in lesions from patients with other chronic inflammatory diseases, but the number of Foxp3؉ Treg in CLE was significantly reduced. There was no correlation between disease subtype and the frequency of Foxp3؉ Treg in the skin of patients with CLE. In peripheral blood, no significant differences were observed in the number and phenotype of CD4؉,CD25؉ Treg or in the sensitivity to apoptosis of CD4؉,CD25 high Treg derived from patients with CLE and those derived from normal healthy donors.Conclusion. These data suggest that an organspecific abnormality of Treg in the skin underscores the importance of analyzing Treg in the affected tissue. Such a local process might give insight into the pathogenic mechanisms of CLE and differs from a global peripheral dysfunction as reported for patients with a systemic manifestation of the disease.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Low number of regulatory T cells in skin lesions of patients with cutaneous lupus erythematosus

Franz¹,

Fritzsching²,

Riehl³

et al. 2007

Arthritis & Rheumatism

123

View full text Add to dashboard Cite

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“…In particular, cytokines involved in Th1 differentiation may have a very critical role. A typical phenotype in IBDs in either human or mouse, is an exaggerated Th1 type response with excess generation of IFN-γ and TNF-α, which may result in tissue injury [33,[49][50][51][52][53][54]. Th1 cells are induced by the differentiation of activated Tcells in the presence of IL-12.…”

Section: The Stat Signaling Pathwaymentioning

confidence: 99%

“…Th1 cells are induced by the differentiation of activated Tcells in the presence of IL-12. During a balanced response, these effector T-cells are inhibited by IL-10-dependent regulatory T-cells [54]. Th1 cells are a major source of IFN-γ and activate cytotoxic T-cells.…”

Section: The Stat Signaling Pathwaymentioning

confidence: 99%

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STAT3 in immune responses and inflammatory bowel diseases

2006

Cell Res

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214npg STAT3 has been known as a mediator for gene expression induced by many important cytokines. Recent studies have suggested that STAT3 has important functions in regulation of both innate and adaptive immunity. Loss of STAT3 in immune cells caused severe inflammation in response to pathogens. This review discusses the recent progress and suggests directions for the future research on this interesting molecule.

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Inflammatory Bowel Disease

Stenson

Hanauer

Cohen

2008

Textbook of Gastroenterology

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In the US, H. pylori vacA shows allelic variation in the signal sequence (which may be type sla, slb or s2) and the mid-region (type ml or m2). Previous PCR-based vacA mid-region typing classified most, but not all, Asian and South American strains tested as ml or m2. We now sought to investigate vacA mid-region diversity further.MotlmodL We studied 13 Japanese, 6 Chinese, 9 Thai, and 8 Peruvian H. pylori isolates. cagA was identified by colony hybridisation (CH), vacA signal sequence was typed by PCR, and vacA mid-region was typed proximally by CH and distally by PCR. Sections of the vacA mid-region from 8 strains were PCR-amplified, sequenced, and compared with known sequences from 8 other strains.Rcuts Of the 36 Asian and South American strains studied, 35 were cagA+ (the cagA-was Peruvian) and 35 were vacA sla (1 cagA + Peruvian was slb). vacA mid-regions from the 13 Japanese strains were not PCR-amplified by ml or m2-specific primers, but hybridised weakly with an ml probe. Sequence analysis of vacA from 1 Japanese strain revealed 91% nucleotide identity with the ml probe but only 71% identity with the m2 probe. The previously equivocal Thai and Peruvian strains also had ml-like mid-region sequences. A Chinese strain was ml in the proximal mid-region and m2 distally, showing a clear crossover site. Final mid-region types were: Japanese, all 13 ml; Chinese, 1 ml, 1 ml/m2, 4 m2; Thai, 3 ml, 6 m2; Peruvian 4 ml, 4 m2. Distal mid-region sequences of 16 strains, compared over 294 bp, clustered into 2 groups, ml and m2. Nucleotide identity between ml and m2 strains ranged from 73-78%. Within groups, m2 strains were less diverse than ml strains (m2 range 94-99.7%, ml 88-99.3%, p<0.001). Sequence analysis of 7 ml and 3 m2 strains over 1.1kb proximally showed maintenance of clustering outside the 294bp region C duions These Asian and South American strains are similar in terms of cagA status and vacA sla genotype, but fall into 2 vacA midregion groups. ml sequences are more diverse than m2, and thus may be phylogenetically older. The vacA sequence of 1 Chinese strain suggests recombination in vivo between ml and m2 alleles. Introduction: H.pylori antimicrobial susceptibility is an important determinant ofthe efficacy of eradication therapies'. The prevalence of antimicrobial resistance varies within the UK and may increase given the increased use of eradication therapy. This multicentre study assesses the prevalence and possible associations ofH.pylon antimicrobial resistance. Methods: H.pylon was isolated from antral biopsies of patients undergoing routine endoscopy and cultured according to standard microbiological methods. Antimicrobial resistance was determined using "E-tests" or disc tests (tinidazole only) with breakpoints defined by previous studies. Results: H.pyloni was isolated from 32% (1222/3823) of patients and antimicrobial susceptibility determined in 90% (1077/1222) Previous case-control studies of IBD have used hospital or community derived controls. There are obvious inherent biases in both metho...

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Regulatory T cells and intestinal homeostasis

Cited by 253 publications

References 84 publications

Low number of regulatory T cells in skin lesions of patients with cutaneous lupus erythematosus

Low number of regulatory T cells in skin lesions of patients with cutaneous lupus erythematosus

STAT3 in immune responses and inflammatory bowel diseases

Inflammatory Bowel Disease

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