Regulatory Role of Na,K-ATPase in the Growth of Heart Tissue Explants in Organotypic Culture

Lopatina, E. V.; Penniyainen, V. A.; Zaika, A. A.

doi:10.1007/s10517-005-0440-x

Cited by 8 publications

(10 citation statements)

References 4 publications

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“…Ouabain being applied at 1 × 10 -8 М inhibits the growth of cardiac tissue explants almost completely (6). AI value is equal to 5% of the control value.…”

Section: Resultsmentioning

confidence: 90%

“…-10 М) stimulates the growth of cardiac tissue explants by an average of 37% (6). It is the concentration of ouabain that regulates the signal-transducing function of Na…”

Section: Resultsmentioning

confidence: 99%

“…Culture medium рН 7.4: 45% -Hank's solution; 40% -Eagle's medium; 15% -fetal cow serum; supplemented with glucose (0.6%), insulin (0.5 ED/ml), gentomycin (100 ED/ml), glutamine (0.35%) (6).…”

Section: Methodsmentioning

confidence: 99%

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Organotypic tissue culture investigation of homocysteine thiolactone cardiotoxic effect

Lopatina¹,

Av²,

Penniyaynen³

et al. 2015

Acta Physiologica Hungarica

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Homocysteine thiolactone was demonstrated to inhibit the growth of 10–12-day-old chicken embryo cardiac tissue explants at 7 × 10−9 −1 × 10−3 M concentrations in a dose-dependent manner. The maximal cardiotoxic effect of homocysteine thiolactone was detected at 1 × 10−3 M, which corresponds to severe hyperhomocysteinemia. The results of experiments on culturing of cardiac tissue explants in the medium containing homocysteine thiolactone (1 × 10−3 M) and ouabain at concentrations regulating the signal-transducing (1 × 10−10 M) and pumping (1 × 10−8 M) functions of Na+,K+ -ATPase indicate that the cardiotoxic effect of homocysteine thiolactone is supposed to result from inhibition of the Na+,K+ -ATPase pumping function.

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“…Ouabain being applied at 1 × 10 -8 М inhibits the growth of cardiac tissue explants almost completely (6). AI value is equal to 5% of the control value.…”

Section: Resultsmentioning

confidence: 90%

“…-10 М) stimulates the growth of cardiac tissue explants by an average of 37% (6). It is the concentration of ouabain that regulates the signal-transducing function of Na…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Organotypic tissue culture investigation of homocysteine thiolactone cardiotoxic effect

Lopatina¹,

Av²,

Penniyaynen³

et al. 2015

Acta Physiologica Hungarica

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“…The study was carried out on 300 explants of sensory ganglia and 350 heart tissue explants from 10-12-day chicken embryos cultured over 3 days on collagen-coated Petri dishes at 36.5 o C and 5% CO 2 [2][3][4]. The nutrient medium contained 40% Hanks' solution, 40% Eagles' medium, 5% chicken embryo extract, and 15% FCS with insulin (0.5 U/ml), glucose (0.6%), glutamine (2 mM), and gentamicin (100 U/ml).…”

Section: Methodsmentioning

confidence: 99%

“…This enzyme is regarded as a transductor of intracellular signal routes [1,5,7,8]. This function of Na + ,K + -ATPase manifests in modulation of proliferation and growth of cells of different tissues [2][3][4]7,8].…”

mentioning

confidence: 99%

Drug Modulation of Transductor Function of Na+,K+-ATPase

Lopatina¹,

Pennyaynen

Rogachevskyi

et al. 2008

Bull Exp Biol Med

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Experimental proof of the hypothesis on modulation of the transductor function of Na+,K+-ATPase by the ouabain-Ca2+ chelate complex was obtained by the method organotypic tissue culture. Quantum chemical estimations detected two principally different modes of Ca2+ ion chelation by ouabain molecule. It is hypothesized that the ouabain-Ca2+-Na+,K+-ATPase ligand-receptor complex is formed due to ion-ion bonds. The formation of the complex serves as the signal triggering the enzyme transductor function. It is experimentally proven that ouabain is incapable of inhibiting neurite growth in sensory neuron and heart tissue explants after removal of free calcium from the nutrient medium with EGTA.

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