Organotypic tissue culture investigation of homocysteine thiolactone cardiotoxic effect

Abstract

Homocysteine thiolactone was demonstrated to inhibit the growth of 10–12-day-old chicken embryo cardiac tissue explants at 7 × 10−9 −1 × 10−3 M concentrations in a dose-dependent manner. The maximal cardiotoxic effect of homocysteine thiolactone was detected at 1 × 10−3 M, which corresponds to severe hyperhomocysteinemia. The results of experiments on culturing of cardiac tissue explants in the medium containing homocysteine thiolactone (1 × 10−3 M) and ouabain at concentrations regulating the signal-transducing (1 × 10−10 M) and pumping (1 × 10−8 M) functions of Na+,K+ -ATPase indicate that the cardiotoxic effect of homocysteine thiolactone is supposed to result from inhibition of the Na+,K+ -ATPase pumping function.

Homocysteine as a Predictor of Paroxysmal Atrial Fibrillation-Related Events: A Scoping Review of the Literature

“…Furthermore, Hcy has been reported to down-regulate the production of beta 2 adrenergic receptors [ 83 , 84 ], as well as to increase responsiveness to beta-adrenergic agonists [ 85 ]. Finally, alterations involving atrial calcium [ 74 ], sodium [ 77 , 78 , 80 ] and potassium [ 75 , 78 , 79 , 101 ] channels have also been demonstrated in hyperhomocysteinemia.…”

Role of the Rhamnosyl Residue of Ouabain in the Activation of the Na,K-ATPase Signaling Function

“…The objects of the study were DRG, cardiac, retina, skin, and liver tissue explants of 10-12-day old White Leghorn chick embryos obtained as described previously [32,35]. Briefly, explants were cultured in a CO 2 incubator (Sanyo, Osaka, Japan) for 3 days on collagen substrates in Petri dishes at 37 • C and CO 2 .…”