Regulatory network for cell shape changes during Drosophila ventral furrow formation

Aracena, Julio; González, Maurício; Zúñiga, Alejandro; Méndez, Marco A.; Cambiazo, Verónica

doi:10.1016/j.jtbi.2005.07.011

Cited by 35 publications

(26 citation statements)

References 83 publications

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“…8). Invagination of the mesoderm is the first step in this process and is dependent on Snail, Twist, Concertina, Fog and several other genes (Parks and Wieschaus, 1991;Reuter and Leptin, 1994;Morize et al, 1998;Aracena et al, 2006;Seher et al, 2007;Martin et al, 2009). Next, collapse of the mesoderm tube onto the ectoderm requires Htl activation via Ths.…”

Section: Mesoderm Migration Involves Four Distinct Stepsmentioning

confidence: 99%

Mesoderm migration in Drosophila is a multi-step process requiring FGF signaling and integrin activity

et al. 2010

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SUMMARYMigration is a complex, dynamic process that has largely been studied using qualitative or static approaches. As technology has improved, we can now take quantitative approaches towards understanding cell migration using in vivo imaging and tracking analyses. In this manner, we have established a four-step model of mesoderm migration during Drosophila gastrulation: (I) mesodermal tube formation, (II) collapse of the mesoderm, (III) dorsal migration and spreading and (IV) monolayer formation. Our data provide evidence that these steps are temporally distinct and that each might require different chemical inputs. To support this, we analyzed the role of fibroblast growth factor (FGF) signaling, in particular the function of two Drosophila FGF ligands, Pyramus and Thisbe, during mesoderm migration. We determined that FGF signaling through both ligands controls movements in the radial direction. Thisbe is required for the initial collapse of the mesoderm onto the ectoderm, whereas both Pyramus and Thisbe are required for monolayer formation. In addition, we uncovered that the GTPase Rap1 regulates radial movement of cells and localization of the beta-integrin subunit, Myospheroid, which is also required for monolayer formation. Our analyses suggest that distinct signals influence particular movements, as we found that FGF signaling is involved in controlling collapse and monolayer formation but not dorsal movement, whereas integrins are required to support monolayer formation only and not earlier movements. Our work demonstrates that complex cell migration is not necessarily a fluid process, but suggests instead that different types of movements are directed by distinct inputs in a stepwise manner.

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Section: Mesoderm Migration Involves Four Distinct Stepsmentioning

confidence: 99%

Mesoderm migration in Drosophila is a multi-step process requiring FGF signaling and integrin activity

et al. 2010

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“…A more advanced model will be developed to include the spatiotemporal dynamics of the most important genes that mediate the cellular response to Dorsal signal (Aracena et al 2006). This improvement would permit to perform simulations corresponding to some experiences in mutants (lacking of some genes expression).…”

Section: Discussionmentioning

confidence: 99%

A General Formalism for Tissue Morphogenesis Based on Cellular Dynamics and Control System Interactions

Forest

Demongeot

2008

Acta Biotheor

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Morphogenesis is a key process in developmental biology. An important issue is the understanding of the generation of shape and cellular organisation in tissues. Despite of their great diversity, morphogenetic processes share common features. This work is an attempt to describe this diversity using the same formalism based on a cellular description. Tissue is seen as a multi-cellular system whose behaviour is the result of all constitutive cells dynamics. Morphogenesis is then considered as a spatiotemporal organization of cells activities. We show how this formalism relies on Reaction-Diffusion/Positional Information approach and how it permits to generalize its modelling possibilities. Three quite different applications for concrete morphogenetic processes are presented. The first one is a model for epithelial invagination, the second is a model of cellular differentiation by local cell-cell signalling. The last example is the secondary radial growth of conifer trees. From the mathematical point of view, different modelling tools are used according to the specificity of each process.L. Forest (

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“…A number of statistical and computational methods have been applied in the modeling of gene regulation networks [58][59][60][61][62][63][64]. A few regulatory networks have been defined [60,[65][66][67][68]. Despite this, regulatory network construction remains a great challenge due to the requirement of large experimental data sets.…”

Section: Bioinformatics and Functional Genomicsmentioning

confidence: 99%

High‐Throughput Technologies and Functional Genomics

Wan

Thompson

2009

Systems Biology and Synthetic Biology

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Regulatory network for cell shape changes during Drosophila ventral furrow formation

Cited by 35 publications

References 83 publications

Mesoderm migration in Drosophila is a multi-step process requiring FGF signaling and integrin activity

Mesoderm migration in Drosophila is a multi-step process requiring FGF signaling and integrin activity

A General Formalism for Tissue Morphogenesis Based on Cellular Dynamics and Control System Interactions

High‐Throughput Technologies and Functional Genomics

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