Regulatory mechanism of Toona sinensis on mouse leydig cell steroidogenesis

Poon, Song Ling; Leu, Sew Fen; Hsu, Hseng Kuang; Liu, Ming Yie; Huang, Bu Miin

doi:10.1016/j.lfs.2004.08.026

Cited by 51 publications

(11 citation statements)

References 15 publications

(14 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, we found that cAMP stimulates testosterone production in primary mouse Leydig cells, which is consistent with results of previous studies [28–30]. However, cotreatment with IA (0.5, 1, or 4 h) attenuated cAMP-stimulated testosterone production (Figure 2), consistent with results obtained with primary bovine luteum cells, suggesting that IA rapidly inhibits steroidogenesis in murine Leydig cells.…”

Section: Discussionsupporting

confidence: 93%

Molecular Mechanism of Isocupressic Acid Supresses MA-10 Cell Steroidogenesis

Tsui

Wang

et al. 2012

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Consumption of ponderosa pine needles causes late-term abortions in cattle and is a serious poisonous plant problem in foothill and mountain rangelands. Isocupressic acid (IA) is the component of pine needles responsible for the abortifacient effect, its abortifacient effect may be due to inhibition of steroidogenesis. To investigate the more detail molecular mechanism, we used MA-10 cell, which is wild used to investigate molecular mechanism of steroidogenesis, to characterize the molecular mechanisms underlying the actions of IA in more detail. In this report, we focus on the function of IA on important steroidogenic genes, including steroidogenic acute regulatory protein (StAR), cytochrome P450 cholesterol side-chain cleavage (P450scc), and 3β-hydroxysteroid dehydrogenase (3β-HSD). We found that IA does not affect enzyme activities of these genes but inhibits transcription of P450scc and translation of StAR and P450scc through attenuating cAMP-PKA signaling. Thus, steroid productions of cells were suppressed.

show abstract

Section: Discussionsupporting

confidence: 93%

Molecular Mechanism of Isocupressic Acid Supresses MA-10 Cell Steroidogenesis

Tsui

Wang

et al. 2012

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…Suppression of the activities of steroidogenic enzymes including the P450 side-chain cleavage enzyme, 3 β-hydroxysteroid dehydrogenase, 17 α-hydroxylase, 20 α-hydroxylase and 17 β-hydroxysteroid dehydrogenase, was observed when primary mouse Leydig cells were incubated with varying concentrations of crude Toona sinensis [45]. The leaves of Azadirachta indica when administered orally at a dose of 500 mg per kg body weight exhibited a regression and decrease in the number of Leydig cells and their nuclear diameter, indicating androgen deficiency[46].…”

Section: Steroidogenesis and Medicinal Plantsmentioning

confidence: 99%

The potentiality of medicinal plants as the source of new contraceptive principles in males

et al. 2011

View full text Add to dashboard Cite

Rising human population throughout the world especially in developing and underdeveloped countries has detrimental effects on life supporting system on earth. Traditionally, plants have been used to treat different kinds of ailments. The growing importance of phytochemicals in males has been reported. Contraceptive ability of plants has been reported in several animal models. The reversibility of the anti-fertility effects of plants and its active compounds are of potential clinical relevance in the development of male contraceptive. This review attempts to discuss the latest reports on the potentiality of medicinal plants as the source of new contraceptive principles in males.

show abstract

“…Importantly, almost all parts of T. sinensis including seed, bark, root bark, petioles, and leaves have a number of traditional Chinese medicinal values (for example: used as febrifuge, astringent, carminative, and used for enteritis and dysentery treatments) (Xien, 1996;Edmonds and Staniforth, 1998). More recently, various other biological values discovered from the T. sinensis leaf extracts have been reported: anti-cancer (Chang et al, 2002;Wang et al, 2010;Yang et al, 2010), antiinflammatory (Yang et al, 2006), anti-diabetes (Hsu et al, 2003), anti-neoplastic effect (Chia et al, 2010), and inhibitory effects on Leydig cell steroidogenesis (Poon et al, 2005), severe acute respiratory syndromes and coronavirus replication (Chen et al, 2008), and antitumor (Yang et al, 2013). In addition, T. sinensis trunk is a good source of hardwood (Edmonds and Staniforth, 1998).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Toona sinensis Roem in China revealed by ISSR and SRAP markers

et al. 2016

View full text Add to dashboard Cite

ABSTRACT. Toona sinensis Roem has an important value as a type of traditional vegetable and Chinese medicinal herb, and is also a valuable source of wood in China. In this study, we used the inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers to assess the level and pattern of genetic diversity in five domesticated T. sinensis populations in China. Our results indicated a relatively low level of genetic diversity both at species (Hs = 0.1662, 0.2098, respectively) and population levels (Hs = 0.0978, 0.1145, respectively). Molecular variance analyses revealed a relatively high degree of differentiation among populations (G ST = 0.3901, 0.4498), and low levels of gene flow (N m = 0.7816 and 0.6116). We divided the five populations into two groups by cluster analysis: group one consists of populations collected from the south part of China (e.g., Yuxi, Yunan Province and Zuanjiang, Chongqing Municipality), and group two contains those cultivated in north part of China (e.g., Hengshui, Hebei Province, Jinan and Rizhao, Shandong Province). The correlation of genetic relationships among populations fits well with their geographical distribution (Mantel test; r = 0.7236 and 0.6789, respectively). Asexual propagation, limited gene flow and geographic isolation are most likely the key factors associated with the observed genetic structure of T. sinensis grown in China. The present study indicated that both ISSR and SRAP markers were effective and reliable for assessing the degree of T. sinensis genetic variations.

show abstract

Regulatory mechanism of Toona sinensis on mouse leydig cell steroidogenesis

Cited by 51 publications

References 15 publications

Molecular Mechanism of Isocupressic Acid Supresses MA-10 Cell Steroidogenesis

Molecular Mechanism of Isocupressic Acid Supresses MA-10 Cell Steroidogenesis

The potentiality of medicinal plants as the source of new contraceptive principles in males

Genetic diversity of Toona sinensis Roem in China revealed by ISSR and SRAP markers

Contact Info

Product

Resources

About