Regulatory function of sigma factors RpoS/RpoN in adaptation and spoilage potential of Shewanella baltica

Feng, Lili; Bi, Weiwei; Chen, Shuai; Zhu, Junli; Liu, Xiaoxiang

doi:10.1016/j.fm.2021.103755

Cited by 33 publications

(25 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By sensing the existence of AI-2 signals, the quantitative growth and the metabolic process of S. baltica could be inhibited. Although the growth and metabolism of SS-128 might use L. vannamei as an energy source, its damage to L. vannamei was far less than that from S. baltica , which is a recognized spoilage microorganism for aquatic products ( Feng et al, 2021 ). The AI-2/LuxS QC system of SS-128 retarded the damage of texture proteins from microorganisms, especially from S. baltica .…”

Section: Resultsmentioning

confidence: 99%

LuxS in Lactobacillus plantarum SS-128 Improves the Texture of Refrigerated Litopenaeus vannamei: Mechanism Exploration Using a Proteomics Approach

Qian

Lou

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

This study illustrated the texture changes of Shewanella baltica-inoculated Litopenaeus vannamei during refrigerated storage with the exogenous addition of Lactobacillus plantarum SS-128. The group inoculated with SS-128 had an improved texture compared with that inoculated with the luxS-mutant group (ΔluxS). Proteomics were conducted to analyze the protein alterations in L. vannamei and supernatant, respectively. During storage, many texture-related proteins, including myosin heavy chain and beta-actin, were maintained due to luxS. Some endogenous enzymes related to the energy metabolism and hydrolysis of L. vannamei were downregulated. The luxS-induced interaction with S. baltica showed significant changes in the expression of some critical enzymes and pathways. The ATP-dependent zinc metalloprotease FtsH and protease subunit HslV were downregulated, and the oxidative phosphorylation and glycosaminoglycan degradation pathways in S. baltica were inhibited, resulting in the slow deterioration of L. vannamei. By exploring the mechanism underlying SS-128-led manipulation of the metabolism of spoilage bacteria, we clarified the texture maintenance mechanism of luxS in SS-128, providing theoretical evidence for SS-128 application in food preservation.

show abstract

Section: Resultsmentioning

confidence: 99%

LuxS in Lactobacillus plantarum SS-128 Improves the Texture of Refrigerated Litopenaeus vannamei: Mechanism Exploration Using a Proteomics Approach

Qian

Lou

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…The results showed that there was a significant positive correlation between the adhesion ability of S. putrefaciens and spoilage ability. TVB-N mainly includes trimethylamine, dimethylamine, ammonia, and other nitrogen-containing substances formed by the protein decomposition of the fish due to the action of enzymes and bacteria [29]. However, it is not absolute to use the total number of colonies and TVB-N to reflect the spoilage capacity of a strain, and the spoilage capacity of a strain could be comprehensively reflected by calculating the yield factor of spoilage.…”

Section: Discussionmentioning

confidence: 99%

The Adhesion and Spoilage of Shewanella putrefaciens in Tilapia

Zhang

et al. 2022

Foods

View full text Add to dashboard Cite

Shewanella putrefaciens is a typical spoilage bacteria organism in seafood. The adhesion ability of three S. putrefaciens strains (HR-15, JR-18, HC-71) isolated from putrefied tilapia were evaluated by mucus adhesion in vitro and intestinal adhesion in vivo. The results of the spoilage of the inoculated fish fillets and spoilage of the refrigerated fish both showed that the adhesion ability of S. putrefaciens was positively correlated with the spoilage ability. High-throughput sequencing and GC-MS results showed that S. putrefaciens with high adhesion ability also significantly changed the intestinal flora of fish, causing an increase in the intestinal bacteria such as Plesionomas, Macellibacteroides, Acinetobacter, and Legionella, which then led to the increase in volatile substances such as low-grade aldehydes, alcohols, and ketones in the fish, serious fatty acid oxidation, and excitement of the fishy smell.

show abstract

“…The exopolysaccharide of the inoculated fish juice was extracted according to the method of Feng et al [24]. Nine milliliters of the inoculated fish juice was transferred to a six-well plate and incubated at 30 • C for 24, 48, and 72 h and at 4 • C for 1, 2, and 4 days, respectively.…”

Section: Determination Of Exopolysaccharide Content and Extracellular...mentioning

confidence: 99%

Genomic Analysis of Two Representative Strains of Shewanella putrefaciens Isolated from Bigeye Tuna: Biofilm and Spoilage-Associated Behavior

Xie

2022

Foods

View full text Add to dashboard Cite

Shewanella putrefaciens can cause the spoilage of seafood and shorten its shelf life. In this study, both strains of S. putrefaciens (YZ08 and YZ-J) isolated from spoiled bigeye tuna were subjected to in-depth phenotypic and genotypic characterization to better understand their roles in seafood spoilage. The complete genome sequences of strains YZ08 and YZ-J were reported. Unique genes of the two S. putrefaciens strains were identified by pan-genomic analysis. In vitro experiments revealed that YZ08 and YZ-J could adapt to various environmental stresses, including cold-shock temperature, pH, NaCl, and nutrient stresses. YZ08 was better at adapting to NaCl stress, and its genome possessed more NaCl stress-related genes compared with the YZ-J strain. YZ-J was a higher biofilm and exopolysaccharide producer than YZ08 at 4 and 30 °C, while YZ08 showed greater motility and enhanced capacity for biogenic amine metabolism, trimethylamine metabolism, and sulfur metabolism compared with YZ-J at both temperatures. That YZ08 produced low biofilm and exopolysaccharide contents and displayed high motility may be associated with the presence of more a greater number of genes encoding chemotaxis-related proteins (cheX) and low expression of the bpfA operon. This study provided novel molecular targets for the development of new antiseptic antisepsis strategies.

show abstract

Regulatory function of sigma factors RpoS/RpoN in adaptation and spoilage potential of Shewanella baltica

Cited by 33 publications

References 43 publications

LuxS in Lactobacillus plantarum SS-128 Improves the Texture of Refrigerated Litopenaeus vannamei: Mechanism Exploration Using a Proteomics Approach

LuxS in Lactobacillus plantarum SS-128 Improves the Texture of Refrigerated Litopenaeus vannamei: Mechanism Exploration Using a Proteomics Approach

The Adhesion and Spoilage of Shewanella putrefaciens in Tilapia

Genomic Analysis of Two Representative Strains of Shewanella putrefaciens Isolated from Bigeye Tuna: Biofilm and Spoilage-Associated Behavior

Contact Info

Product

Resources

About