Regulatory Cells and Cytokines Involved in Primary B Lymphocyte Production

Dorshkind, Kenneth; Narayanan, Ramaswamy; Landreth, Kenneth S.

doi:10.1007/978-1-4615-3396-2_15

Cited by 16 publications

(5 citation statements)

References 20 publications

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“…Therefore PBEF might play an active role in increasing the number of B220+ cells for IL-7 to act on. The presence of an activity such as PBEF has been predicted before (9,13), and in our assay conditions, the addition of insulin-like growth factor (IGF) did not substitute for the presence of PBEF.…”

Section: Resultssupporting

confidence: 52%

“…Primitive cells, defined as lineage negative/Sca-1 positive (Lin-l-Sca-l+), do not respond to SCF plus IL-7, but in the presence of SCF plus IL-3 and stromal cells, Lin-1-Sca-l+ cells generate B220+ cells (16a). Detailed studies by Landreth et al (9,13) suggest that additional stromal-cell-derived but not-yet-characterized cytokines control the developmental step in which the maturation of immature B-cell precursors to an IL-7-SCFresponsive state occurs. In this report, we demonstrate that the effect of SCF and IL-7 on murine pre-B-cell colony formation can be significantly enhanced by a novel cytokine-like molecule. This molecule is the product of a novel gene which is expressed in bone marrow stromal cells as well as in activated human lymphocytes.…”

mentioning

confidence: 99%

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Cloning and characterization of the cDNA encoding a novel human pre-B-cell colony-enhancing factor.

Samal¹,

Sun²,

Stearns³

et al. 1994

Mol. Cell. Biol.

898

799

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Section: Resultssupporting

confidence: 52%

mentioning

confidence: 99%

Cloning and characterization of the cDNA encoding a novel human pre-B-cell colony-enhancing factor.

Samal¹,

Sun²,

Stearns³

et al. 1994

Mol. Cell. Biol.

898

799

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 72%

Cloning and Characterization of the cDNA Encoding a Novel Human pre-B-Cell Colony-enhancing Factor

Samal

Sun

Stearns

et al. 1994

Molecular and Cellular Biology

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A novel gene coding for the pre-B-cell colony-enhancing factor (PBEF) has been isolated from a human peripheral blood lymphocyte cDNA library. The expression of this gene is induced by pokeweed mitogen and superinduced by cycloheximide. It is also induced in the T-lymphoblastoid cell line HUT 78 after phorbol ester (phorbol myristate acetate) treatment. The predominant mRNA for PBEF is approximately 2.4 kb long and codes for a 52-kDa secreted protein. The 3' untranslated region of the mRNA has multiple TATT motifs, usually found in cytokine and oncogene messages. The PBEF gene is mainly transcribed in human bone marrow, liver tissue, and muscle. We have expressed PBEF in COS 7 and PA317 cells and have tested the biological activities of the conditioned medium as well as the antibody-purified protein in different in vitro assays. PBEF itself had no activity but synergized the pre-B-cell colony formation activity of stem cell factor and interleukin 7. In the presence of PBEF, the number of pre-B-cell colonies was increased by at least 70% above the amount stimulated by stem cell factor plus interleukin 7. No effect of PBEF was found with cells of myeloid or erythroid lineages. These data define PBEF as a novel cytokine which acts on early B-lineage precursor cells.

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“…For instance, precursor B cells/Pro-B cells production is greatly reduced when there is a decreased amount of mRNA of the recombination activating genes 1 and 2 in aged bone marrow [ 42 , 45 ]. Moreover, signals from stromal cells have been shown to potentiate the effect of other gene groups, such as the interleukin 7 receptor, that modulate the proliferation and differentiation of developing B cells [ 46 , 47 , 48 , 49 , 50 ]. These events may require direct cell communication with stromal cells and biological responses to signaling growth factors [ 51 , 52 ].…”

Section: Discussionmentioning

confidence: 99%

Bone Marrow-Derived Stem Cell Populations Are Differentially Regulated by Thyroid or/and Ovarian Hormone Loss

Mogharbel¹,

Abdelwahid

Irioda³

et al. 2017

IJMS

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Bone marrow-derived stem cells (BMDSCs) play an essential role in organ repair and regeneration. The molecular mechanisms by which hormones control BMDSCs proliferation and differentiation are unclear. Our aim in this study was to investigate how a lack of ovarian or/and thyroid hormones affects stem cell number in bone marrow lineage. To examine the effect of thyroid or/and ovarian hormones on the proliferative activity of BMDSCs, we removed the thyroid or/and the ovaries of adult female rats. An absence of ovarian and thyroid hormones was confirmed by Pap staining and Thyroid Stimulating Hormone (TSH) measurement, respectively. To obtain the stem cells from the bone marrow, we punctured the iliac crest, and aspirated and isolated cells by using a density gradient. Specific markers were used by cytometry to identify the different BMDSCs types: endothelial progenitor cells (EPCs), precursor B cells/pro-B cells, and mesenchymal stem cells (MSCs). Interestingly, our results showed that hypothyroidism caused a significant increase in the percentage of EPCs, whereas a lack of ovarian hormones significantly increased the precursor B cells/pro-B cells. Moreover, the removal of both glands led to increased MSCs. In conclusion, both ovarian and thyroid hormones appear to have key and diverse roles in regulating the proliferation of cells populations of the bone marrow.

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Regulatory Cells and Cytokines Involved in Primary B Lymphocyte Production

Cited by 16 publications

References 20 publications

Cloning and characterization of the cDNA encoding a novel human pre-B-cell colony-enhancing factor.

Cloning and characterization of the cDNA encoding a novel human pre-B-cell colony-enhancing factor.

Cloning and Characterization of the cDNA Encoding a Novel Human pre-B-Cell Colony-enhancing Factor

Bone Marrow-Derived Stem Cell Populations Are Differentially Regulated by Thyroid or/and Ovarian Hormone Loss

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