Regulation on the biocompatibility of bioactive titanium metals by type I collagen

Wang, Q. Q.; Li, W.; Yang, Bangcheng

doi:10.1002/jbm.a.33142

Cited by 16 publications

(14 citation statements)

References 27 publications

(24 reference statements)

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“…Biomedical titanium has been made into bone devices such as bone plates, bone screws and artificial hip joint for many years . After implanted in vivo , the similar interaction between surface modified titanium and the rhBMP‐2 protein takes place adjacent to the host bone, which greatly influences the new bone formation.…”

Section: Discussionmentioning

confidence: 99%

Regulating the osteogenic function of rhBMP 2 by different titanium surface properties

Xiao

Biao

Chen

et al. 2016

J. Biomed. Mater. Res.

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Bone morphogenetic protein 2 (BMP-2) is important for regulating the osteogenic differentiation of mesenchymal stem cells and the response of bone tissue. It adsorbs on the surface of biomedical implants immediately and plays a role of mediator between the materials surfaces and the host cells. Studies usually connect the material surface properties and the new bone formation directly. However, interaction between the adsorbed BMP-2 on the implant surface and the cells in the tissue is the key to explaining the osteogenic properties of the material. So, in this article, we investigated the conformational and functional changes induced by the surface modified titanium metals. We found that the α-helix and β-sheet structure of rhBMP-2 can be well maintained on the anodic oxidation treated titanium surface. The osteogenic function of rhBMP-2 can sustain for a relatively long time even though there is less amount adhere to the surface compared with that on the acid alkali treated titanium. Surface properties, especially the morphology enable a larger amount of rhBMP-2 to adsorb to the surface of the acid alkali treated titanium, but the conformation of the protein is severely influenced. The percentage of α-helix structure is also significantly decreased so that the efficacy of rhBMP-2 is only maintained in the early time. This study indicated that different surface modification of the surface could regulate the structure of rhBMP-2 and then further influence its osteogenic function. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1882-1893, 2016.

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Section: Discussionmentioning

confidence: 99%

Regulating the osteogenic function of rhBMP 2 by different titanium surface properties

Xiao

Biao

Chen

et al. 2016

J. Biomed. Mater. Res.

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“…Type I collagen, one of the main components of bone extracellular matrix, is an intriguing candidate for surface immobilization because it can modulate various aspects of cell behaviors, such as controlling cell migration and attachment by interacting integrin located on cell membrane . Early reports have shown that type I collagen immobilized on Ti‐based implants can modulate various aspects of cell behavior . It can be hypothesized that the biological function of titanium coating would also be improved by immobilizing type I collagen.…”

Section: Introductionmentioning

confidence: 99%

“…In adsorptive immobilization, collagen is physically adsorbed on the surface through van der Waals, hydrophobic or electrostatic forces . The surface characteristics of Ti‐based implants, such as the wettability, electric charge, and hydrophilicity, influence the absorption of collagen . In covalent immobilization, collagen can be covalently bound onto titanium surfaces using a variety of cross‐linking agents, such as aminopropylsilane, glutaraldehyde or carbodiimide .…”

Section: Introductionmentioning

confidence: 99%

Improved hMSC functions on titanium coatings by type I collagen immobilization

Xie

Tan

et al. 2013

J. Biomed. Mater. Res.

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In this study, type I collagen was fixed onto plasma-sprayed porous titanium coatings by either adsorptive immobilization or covalent immobilization. Surface characterization by scanning electron microscopy (SEM), diffuse reflectance Fourier transform infrared spectroscopy (DR-FTIR) and X-ray photoelectron spectroscopy (XPS) confirmed the biochemical modification of the titanium coatings. The immobilizing effects of type I collagen, including variations in the amount and stability of collagen, were investigated using Sirius red staining. A greater amount of collagen was found on the covalently immobilized titanium coating, and higher stability was achieved relative to the absorptive immobilization surface. Human mesenchymal stem cells (hMSCs) were used to evaluate the cytocompatibility of the modified titanium coatings. Type I collagen immobilized on titanium coating led to enhance cell-material interactions and improved hMSC functions, such as attachment, proliferation, and differentiation. Interestingly, covalently immobilized collagen on titanium coating showed a greater capability to regulate the osteogenic activity of hMSCs than did absorbed collagen, which was explained in terms of the increased amount and higher stability of the covalently linked collagen. The type I collagen covalently immobilized titanium coatings with improved biological function may exhibit better osteointegration in clinical application.

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“…Xia et al 11 obtained HA/COL composite coating by co-precipitation of type I collagen and apatite in a collagen-containing modified simulated body fluid, proving that addition of collagen promoted osteoblast activity, which can lead to early bone formation. Wang et al 12 directly absorbed collagen onto the titanium surface after alkali heat treatment, then immersed it in the simulated body fluid to deposit phosphorite. It turned out that collagen addition improved the biocompatibility of the titanium substrate surface and promoted cell activity.…”

Section: Introductionmentioning

confidence: 99%

Effect of osteoblast activity of porous titanium with HA-coating by adding human-like collagen

Kou

et al. 2014

Materials Research Innovations

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In this article, osteoblast activity on two functionalised hydroxyapatite (HA) and hydroxyapatite/ collagen (HAzCOL) surface layers is studied, and the effects of the concentrations of collagen and the collagen adding method on osteoblast activity are investigated. The morphology and elements of the HA and HAzCOL on porous titanium are observed using a scanning electron microscope and an energy dispersive spectrometer. The number of living osteoblasts is found by comparing the optical density value against a control group. The experimental results showed that osteoblasts have different responses at HA and HAzCOL surfaces that have different chemical compositions. The different concentrations and the means of collagen addition can all significantly improve the survival rate of the osteoblasts, but a 7 mg mL 21 concentration of the added amount of collagen in cell activity gives the best result. It can be concluded that collagen molecules deposit on the HAcoating uniformly and provide a favourable position for the osteoblasts.

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Regulation on the biocompatibility of bioactive titanium metals by type I collagen

Cited by 16 publications

References 27 publications

Regulating the osteogenic function of rhBMP 2 by different titanium surface properties

Regulating the osteogenic function of rhBMP 2 by different titanium surface properties

Improved hMSC functions on titanium coatings by type I collagen immobilization

Effect of osteoblast activity of porous titanium with HA-coating by adding human-like collagen

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