Effect of osteoblast activity of porous titanium with HA-coating by adding human-like collagen

Abstract

In this article, osteoblast activity on two functionalised hydroxyapatite (HA) and hydroxyapatite/ collagen (HAzCOL) surface layers is studied, and the effects of the concentrations of collagen and the collagen adding method on osteoblast activity are investigated. The morphology and elements of the HA and HAzCOL on porous titanium are observed using a scanning electron microscope and an energy dispersive spectrometer. The number of living osteoblasts is found by comparing the optical density value against a control group. The experimental results showed that osteoblasts have different responses at HA and HAzCOL surfaces that have different chemical compositions. The different concentrations and the means of collagen addition can all significantly improve the survival rate of the osteoblasts, but a 7 mg mL 21 concentration of the added amount of collagen in cell activity gives the best result. It can be concluded that collagen molecules deposit on the HAcoating uniformly and provide a favourable position for the osteoblasts.

Exploring the potential of the recombinant human collagens for biomedical and clinical applications: a short review

“…However, except applications in hemostatic sponge, skin injury treatments, and skincare [104,105], most of the HLCs applications still remain in the stage of animal testing, such as vascular scaffolds engineering [77][78][79][80], cartilage [81,82] and bone defect repair [83][84][85][86][87][88][89][90], and other diseases treatment [106][107][108][109][110][111].…”

“…Considering all the benefits and its cost-efficient in the production process, human-like collagen if successful certainly has great advantage than its animal extracted counterparts. As a consequence , sufficient supplement of HLC is available and make it possible for us to explore its various applications, including skin injury treatments [71][72][73][74][75][76], vascular scaffolds engineering [77][78][79][80], cartilage [81,82] and bone defect repair [83][84][85][86][87][88][89][90], and haemostatic sponge [28,91,92] etc.…”

“…In early studies, the HLC was not able to be used alone but as a coating to the scaffold due to its poor mechanical strength. For example, HLCs are usually used to coat or mix with the calcium phosphate ceramics, metals, or polymers to improve the compatibility and remain the stiffness of the substrate scaffold [79,80,82,87,88]. In order to increase the strength of the HLC to meet the requirement of the scaffold, compatible cross-link agents were used.…”

“…There are many publications based on HLC added with tough component and used in bone repairing. Calcium phosphate ceramics, metals, or synthetic polymers, such as HA, Ti, NiTi alloy and PLA are usually used to composite scaffolds for bone defect repair [83][84][85][86][87][88][89][90]. For example, NiTi shape memory alloy was used as the basic scaffold.…”

“…Significantly promoted survival of the osteoblasts by the HA/HLC surface layer compared with the HA surface layer [88] HLC and BMP-2 complex A large area of defective bone repair HLC sponge loaded with tiny BMP-2 of 1 µg, with profiles of a controlled release characterized by a short burst and a steady slow process. self-Osteogenesis in situ consequently facilitated with the ability of complete repair of large areas of defective bone…”