Regulation of Yersinia pseudotuberculosis major porin expression in response to antibiotic stress

Bystritskaya, Evgeniya; Stenkova, Anna; Portnyagina, O. Yu.; Rakin, Alexander; Rasskazov, V. A.; Isaeva, Marina

doi:10.3103/s0891416814020037

Cited by 5 publications

(6 citation statements)

References 26 publications

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“…The recombinant plasmids pACYC184-F-GFP and pACYC184-C-GFP containing the Y. pseudotuberculosis ompF and ompC regulatory regions fused to GFP were constructed as previously described [ 7 ]. To obtain the coding region of GFP, the plasmid DNA pTurboGFP (Evrogen, Moscow, Russia) was digested with Bam HI and Hind III (Fermentas, Waltham, MA, USA), and the resulting gfp fragment was dephosphorylated with CAP alkaline phosphatase (Fermentas, Waltham, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

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Differential Expression of Yersinia pseudotuberculosis General Porin Genes during Short- and Long-Term Antibiotic Stresses

et al. 2021

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Here, we investigated general porin regulation in Yersinia pseudotuberculosis 488, the causative agent of Far Eastern scarlet-like fever, in response to sublethal concentrations of antibiotics. We chose four antibiotics of different classes and measured gene expression using qRT-PCR and GFP reporter systems. Our data showed temporal regulation of the general porin genes ompF and ompC caused by antibiotic stress. The porin transcription initially decreased, providing early defensive response of the bacterium, while it returned to that of the untreated cells on prolonged antibiotic exposure. Unlike the major porin genes, the transcription of the alternative porin genes ompX and lamB was increased. Moreover, a short-term ompR- and marA-mediated porin regulation was observed. The main finding was a phenotypic heterogeneity of Y. pseudotuberculosis population manifested in variable porin gene expression under carbenicillin exposure. This may offer adaptive fitness advantages for a particular bacterial subpopulation.

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Section: Methodsmentioning

confidence: 99%

“…Y. pseudotuberculosis produces two major nonspecific porins, OmpF and OmpC, which we study intensively [ 7 , 8 , 9 , 10 , 11 ]. These porins consist of 16-stranded β-barrel trimers, each of which forms a central channel [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Differential Expression of Yersinia pseudotuberculosis General Porin Genes during Short- and Long-Term Antibiotic Stresses

et al. 2021

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“…The balance of major OMPs, known as OmpF and OmpC, is achieved through its complex regulation often reciprocal in response to many environmental factors, such as temperature, osmotic pressure, and pH (Liu and Ferenci 2001). Alteration in the porin balance has been observed for a number of clinical isolates making contribution to the adaptive bacterial response to the treatment (Pagès et al 2008;Bystritskaya et al 2014). In addition to major general porins, there are minor ones expressed at very low levels (Prilipov et al 1998).…”

Section: Original Researchmentioning

confidence: 99%

“…; Bystritskaya et al. ). In addition to major general porins, there are minor ones expressed at very low levels (Prilipov et al.…”

Section: Introductionmentioning

confidence: 98%

Adaptive responses of outer membrane porin balance of Yersinia ruckeri under different incubation temperature, osmolarity, and oxygen availability

et al. 2016

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The capability of Yersinia ruckeri to survive in the aquatic systems reflects its adaptation (most importantly through the alteration of membrane permeability) to the unfavorable environments. The nonspecific porins are a key factor contributing to the permeability. Here we studied the influence of the stimuli, such as temperature, osmolarity, and oxygen availability on regulation of Y. ruckeri porins. Using qRT‐PCR and SDS‐PAGE methods we found that major porins are tightly controlled by temperature. Hyperosmosis did not repress OmpF production. The limitation of oxygen availability led to decreased expression of both major porins and increased transcription of the minor porin OmpY. Regulation of the porin balance in Y. ruckeri, in spite of some similarities, diverges from that system in Escherichia coli. The changes in porin regulation can be adapted in Y. ruckeri in a species‐specific manner determined by its aquatic habitats.

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“…[5][6][7][8] The reciprocal expression of two general porins, OmpC and OmpF, is one of the common ways that bacterial cells adapt to changing environmental conditions. [9][10][11] For example, OmpF (which has a large pore size) is mainly expressed at a temperature of 4 °C-25 °C and when there is a lack of nutrients in the medium, while OmpC (which has a smaller pore size) is primarily expressed at the temperature of a warm-blooded organism (37 °C) and during nutrient excess, 12 OmpC plays a more significant role in bile resistance than OmpF. 13 Therefore, these proteins may contribute to the adaptation of bacterial pathogens to different hosts and different environmental conditions.…”

Section: Introductionmentioning

confidence: 99%

Molecular Evolution of theYersiniaMajor Outer Membrane Protein C (OmpC)

Stenkova

Bystritskaya²,

Guzev

et al. 2016

Evolutionary Bioinformatics

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The genus Yersinia includes species with a wide range of eukaryotic hosts (from fish, insects, and plants to mammals and humans). One of the major outer membrane proteins, the porin OmpC, is preferentially expressed in the host gut, where osmotic pressure, temperature, and the concentrations of nutrients and toxic products are relatively high. We consider here the molecular evolution and phylogeny of Yersinia ompC. The maximum likelihood gene tree reflects the macroevolution processes occurring within the genus Yersinia. Positive selection and horizontal gene transfer are the key factors of ompC diversification, and intraspecies recombination was revealed in two Yersinia species. The impact of recombination on ompC evolution was different from that of another major porin gene, ompF, possibly due to the emergence of additional functions and conservation of the basic transport function. The predicted antigenic determinants of OmpC were located in rapidly evolving regions, which may indicate the evolutionary mechanisms of Yersinia adaptation to the host immune system.

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Regulation of Yersinia pseudotuberculosis major porin expression in response to antibiotic stress

Cited by 5 publications

References 26 publications

Differential Expression of Yersinia pseudotuberculosis General Porin Genes during Short- and Long-Term Antibiotic Stresses

Differential Expression of Yersinia pseudotuberculosis General Porin Genes during Short- and Long-Term Antibiotic Stresses

Adaptive responses of outer membrane porin balance of Yersinia ruckeri under different incubation temperature, osmolarity, and oxygen availability

Molecular Evolution of theYersiniaMajor Outer Membrane Protein C (OmpC)

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