Adaptive responses of outer membrane porin balance of Yersinia ruckeri under different incubation temperature, osmolarity, and oxygen availability

Abstract

The capability of Yersinia ruckeri to survive in the aquatic systems reflects its adaptation (most importantly through the alteration of membrane permeability) to the unfavorable environments. The nonspecific porins are a key factor contributing to the permeability. Here we studied the influence of the stimuli, such as temperature, osmolarity, and oxygen availability on regulation of Y. ruckeri porins. Using qRT‐PCR and SDS‐PAGE methods we found that major porins are tightly controlled by temperature. Hyperosmosis did not repress OmpF production. The limitation of oxygen availability led to decreased expression of both major porins and increased transcription of the minor porin OmpY. Regulation of the porin balance in Y. ruckeri, in spite of some similarities, diverges from that system in Escherichia coli. The changes in porin regulation can be adapted in Y. ruckeri in a species‐specific manner determined by its aquatic habitats.

Altered Outer Membrane Transcriptome Balance with AmpC Overexpression in Carbapenem-Resistant Enterobacter cloacae

“…It is possible for microorganisms to regulate the OmpF-OmpC ratio, thereby choosing porins with preferable transmembrane channel diameter, electrostatic potential, and the molecular configuration inside the channel. Regulatory cascades can also allow pathogens to take over permeability by the minor porins such as OmpY or OmpK37 (Knopp and Andersson, 2015; Bystritskaya et al, 2016). Various regulatory cascades can be launched in response to changing environmental conditions.…”

Effect of photooxidative stress and mannitol on synthesis of OmpC-OmpF proteins of Escherichia coli in lake water

“…The effects of temperature on effect of the photooxidative stress were examined. E. coli produces two outer membrane proteins, OmpC and OmpF, and these porins play very important roles for survival in aquatic environments [4,17]. Many studies have investigated the effects of light or photosensitizer/light on bacterial survival in aquatic environments [18][19][20].…”

secA, secD, secF, yajC, and yidC contribute to the adhesion regulation of Vibrio alginolyticus

“…Many pathogenic bacteria can induce an adaptable response to environmental stimuli, primarily by altering gene expression (Bystritskaya et al, 2016). Recent study showed that T2SS promotes not only pathogenic potency, but also environmental survival (von Tils, Blädel, Schmidt, & Heusipp, 2012).…”