Regulation of Wnt/β-catenin pathway may be related to Regγ in benign epithelial odontogenic lesions

Santos, Hellen Bandeira de Pontes; Medeiros, Hianne Cristinne De Morais; Mafra, Rodrigo Porpino; Miguel, Márcia Cristina da Costa; Galvão, Hébel Cavalcanti; Souza, Lélia Batista de

doi:10.1016/j.oooo.2018.12.019

Cited by 7 publications

(12 citation statements)

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“…Also, AOTs showed lower levels of matrix metalloproteinases (consistent with a reduced local aggressiveness), Ki76 and anti-apoptosis markers such as Bcl-2, and higher levels of β-catenin (suggesting greater cell adhesion properties) [69]. Similarly, there are publications about the strong cytoplasmatic expression of β-catenin on AOTs [4,21], however no mutation in CTNNB1 was detected [4]. The proposal of Reichart et al [69] was based purely on immunohistochemical findings without considering genetic aspect.…”

Section: Discussionmentioning

confidence: 95%

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Genetic Profile of Adenomatoid Odontogenic Tumor and Ameloblastoma. A Systematic Review

Marín

Niklander

Martínez-Flores

2021

Front. Oral. Health

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Purpose: To perform a comprehensive and systematic critical appraisal of the genetic alterations reported to be present in adenomatoid odontogenic tumor (AOT) compared to ameloblastoma (AM), to aid in the understanding in their development and different behavior.Methods: An electronic search was conducted in PubMed, Scopus, and Web of Science during March 2021. Eligibility criteria included publications on humans which included genetic analysis of AOT or AM.Results: A total of 43 articles reporting 59 AOTs and 680 AMs were included. Different genomic techniques were used, including whole-exome sequencing, direct sequencing, targeted next-generation sequencing panels and TaqMan allele-specific qPCR. Somatic mutations affecting KRAS were identified in 75.9% of all AOTs, mainly G12V; whereas a 71% of the AMs harbored BRAF mutations, mainly V600E.Conclusions: The available genetic data reports that AOTs and AM harbor somatic mutations in well-known oncogenes, being KRAS G12V/R and BRAFV600E mutations the most common, respectively. The relatively high frequency of ameloblastoma compared to other odontogenic tumors, such as AOT, has facilitated the performance of different sequencing techniques, allowing the discovery of different mutational signatures. On the contrary, the low frequency of AOTs is an important limitation for this. The number of studies that have a assessed the genetic landscape of AOT is still very limited, not providing enough evidence to draw a conclusion regarding the relationship between the genomic alterations and its clinical behavior. Thus, the presence of other mutational signatures with clinical impact, co-occurring with background KRAS mutations or in wild-type KRAS cases, cannot be ruled out. Since BRAF and RAS are in the same MAPK pathway, it is interesting that ameloblastomas, frequently associated with BRAFV600E mutation have aggressive clinical behavior, but in contrast, AOTs, frequently associated with RAS mutations have indolent behavior. Functional studies might be required to solve this question.

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Section: Discussionmentioning

confidence: 95%

“…Little information is known about the genetic background of AOT. Mutations in the β-catenin gene ( CTNNB1 ) have been suggested, as strong cytoplasmatic expression of β-catenin is reported using immunohistochemistry [ 4 , 21 ]. However, authors have failed to show alterations in CTNNB1 [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Genetic Profile of Adenomatoid Odontogenic Tumor and Ameloblastoma. A Systematic Review

Marín

Niklander

Martínez-Flores

2021

Front. Oral. Health

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“…34 The expressions of both Wnt-1 and β-catenin were high in solid AB. 35 These aforementioned studies indicate that the canonical Wnt/β-catenin signaling pathway may be related to the occurrence and development of AB. Wnt signaling factors (CTNNB1, APC, AXIN1, and AXIN2) rarely have mutations in AB, 33 which indicates that the abnormal activation of the Wnt/β-catenin signal in AB is not associated with mutations in these genes.…”

Section: Discussionmentioning

confidence: 97%

miR‐29a‐3p promotes migration and invasion in ameloblastoma via Wnt/β‐catenin signaling by targeting catenin beta interacting protein 1

Liu

et al. 2021

Head & Neck

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Background: Ameloblastoma (AB) is a common epithelial odontogenic tumor. The Wnt/β-catenin pathway has been found to be related to AB invasion. Methods:The alteration expression of microRNAs (miRNAs) and messenger RNAs (mRNAs) was performed by miRNA and mRNA microarray analysis and validated by quantitative real-time polymerase chain reaction (RT-PCR). The effects of miR-29a-3p on migration and invasion in AB cells were evaluated by a transwell assay. Bioinformatic prediction was conducted using the miRSystem and validated by quantitative RT-PCR, western blot, and a luciferase reporter assay. Results: miR-29a-3p was overexpressed in AB tissues, which promoted the migration and invasion of AB cells in vitro. Catenin beta interacting protein 1 (CTNNBIP1), a negative regulator of the Wnt/β-catenin pathway, was predicted to be a target of miR-29a-3p. miR-29a-3p inhibited the expression of CTNNBIP1 and promoted the expression of the downstream molecules of the Wnt/β-catenin pathway.Conclusions: miR-29a-3p promoted migration and invasion in AB via Wnt/β-catenin signaling by targeting CTNNBIP1.

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“…En el estudio de Zhang et al (29) , la vía de señalización SHH, que tiene un papel en el desarrollo de los dientes, y su receptor PTC, SMO y el factor de transcripción GLI1 se localizaran predominantemente en el componente epitelial de diferentes tipos de TOs, lo que sugiere su posible participación en la proliferación epitelial de TOs. Aún en este contexto, Santos et al (30) sugirieron la participación de las proteínas Regγ, Wnt-1 y β-catenina en la patogenia de lesiones odontogénicas epiteliales benignas. Finalmente, otros factores potencialmente involucrados en el desarrollo y progresión de los TOs son las moléculas de adhesión celular, las proteínas de degradación de la matriz extracelular, los factores angiogénicos y las citocinas osteolíticas (6) .…”

Section: Principales Aspectos Moleculares Y Genéticos De Los Tumores ...unclassified

Bases moleculares de los tumores odontogénicos benignos: revisión de la literatura en el contexto de la última clasificación de la Organización Mundial de la Salud

2022

Odonto

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Odontogenic tumors (OTs) are a heterogeneous group of lesions that range from hamartomas to benign or malignant neoplasms. The present study aimed to review the literature regarding the molecular and genetic aspects from some benign OTs. Based on the review of the included studies, it is supported that genetic background may play an important role in the etiology and pathogenesis of OTs.However, the body of evidence on the subject still does not allow to conclude the definitive mechanisms involved in the development and progression of OTs. It is suggested the development of further molecular and genetic studies evaluating significant samples of OTs to establish its etiopathogenesis, facilitate its diagnostic process and enrich the therapeutic approaches.

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Regulation of Wnt/β-catenin pathway may be related to Regγ in benign epithelial odontogenic lesions

Cited by 7 publications

References 40 publications

Genetic Profile of Adenomatoid Odontogenic Tumor and Ameloblastoma. A Systematic Review

Genetic Profile of Adenomatoid Odontogenic Tumor and Ameloblastoma. A Systematic Review

miR‐29a‐3p promotes migration and invasion in ameloblastoma via Wnt/β‐catenin signaling by targeting catenin beta interacting protein 1

Bases moleculares de los tumores odontogénicos benignos: revisión de la literatura en el contexto de la última clasificación de la Organización Mundial de la Salud

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