Regulation of transcription factors by sumoylation

Rosonina, Emanuel; Akhter, Akhi; Dou, Yimo; Babu, J. Sreekantha; Theivakadadcham, Veroni S. Sri

doi:10.1080/21541264.2017.1311829

Cited by 102 publications

(111 citation statements)

References 78 publications

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“…These data suggest that conidiation is regulated not only at the transcriptional level, but also at the post‐translational level. This is supported by the fact that SUMO acts as a stabilizer of transcription factors (Rosonina et al ., ). Subsequent identification of SUMOylated motifs in conidiation‐related genes further supports the hypothesis that the modification of conidiation‐related genes by SUMO controls conidiation in M. oryzae .…”

Section: Discussionmentioning

confidence: 97%

SUMOylation is required for fungal development and pathogenicity in the rice blast fungus Magnaporthe oryzae

Lim

Kim

Lee

2018

Molecular Plant Pathology

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Amongst the various post-translational modifications (PTMs), SUMOylation is a conserved process of attachment of a small ubiquitin-related modifier (SUMO) to a protein substrate in eukaryotes. This process regulates many important biological mechanisms, including transcriptional regulation, protein stabilization, cell cycle, DNA repair and pathogenesis. However, the functional role of SUMOylation is not well understood in plant-pathogenic fungi, including the model fungal pathogen Magnaporthe oryzae. In this study, we elucidated the roles of four SUMOylation-associated genes that encode one SUMO protein (MoSMT3), two E1 enzymes (MoAOS1 and MoUBA2) and one E2 enzyme (MoUBC9) in fungal development and pathogenicity. Western blot assays showed that SUMO modification was abolished in all deletion mutants. MoAOS1 and MoUBA2 were mainly localized in the nucleus, whereas MoSMT3 and MoUBC9 were localized in both the nucleus and cytoplasm. However, the four SUMOylation-associated proteins were predominantly localized in the nucleus under oxidative stress conditions. Deletion mutants for each of the four genes were viable, but showed significant defects in mycelial growth, conidiation, septum formation, conidial germination, appressorium formation and pathogenicity. Several proteins responsible for conidiation were predicted to be SUMOylated, suggesting that conidiation is controlled at the post-translational level by SUMOylation. In addition to infection-related development, SUMOylation also played important roles in resistance to nutrient starvation, DNA damage and oxidative stresses. Therefore, SUMOylation is required for infection-related fungal development, stress responses and pathogenicity in M. oryzae. This study provides new insights into the role of SUMOylation in the molecular mechanisms of pathogenesis of the rice blast fungus and other plant pathogens.

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Section: Discussionmentioning

confidence: 97%

SUMOylation is required for fungal development and pathogenicity in the rice blast fungus Magnaporthe oryzae

Lim

Kim

Lee

2018

Molecular Plant Pathology

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“…Such a role could effectively modulate multiple forms of DNA transaction within these structures. In addition, SUMO is well known to regulate individual DNA transaction, such as transcription and DNA repair, by directly targeting proteins involved in these processes; as these topics have been extensively reviewed, we refer readers to some of these articles for details (Rosonina et al, 2017; Sarangi and Zhao, 2015; Schwertman et al, 2016; Wei and Zhao, 2017). …”

Section: Sumo Modulates Chromosome Structures and Functionsmentioning

confidence: 99%

SUMO-Mediated Regulation of Nuclear Functions and Signaling Processes

2018

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Summary Since the discovery of SUMO twenty years ago, SUMO conjugation has become a widely-recognized post-translational modification that targets a myriad of proteins in many processes. Great progress has been made in understanding the SUMO pathway enzymes, substrate sumoylation, and the interplay between sumoylation and other regulatory mechanisms in a variety of contexts. As these research directions continue to generate insights into SUMO-based regulation, several mechanisms by which sumoylation and desumoylation can orchestrate large biological effects are emerging. These include the ability to target multiple proteins within the same cellular structure or process, respond dynamically to external and internal stimuli, and modulate signaling pathways involving other post-translational modifications. Focusing on nuclear function and intracellular signaling, this review highlights a broad spectrum of historical data and recent advances with the aim of providing an overview of mechanisms underlying SUMO-mediated global effects to stimulate further inquiry into intriguing roles of SUMO.

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“…SUMO can impair transcription by promoting the recruitment of transcriptional corepressor complexes or by interfering with transcription-promoting PTMs, such as acetylation and phosphorylation (18). TF SUMOylation is most often associated with reduced target gene expression, which can be mediated by enhanced interactions with corepressors or by interference with protein modifications that promote transcription (19,20).…”

Section: Sumoylation and Gene Transcriptionmentioning

confidence: 99%

“…However, it is becoming evident that SUMOylation also regulates gene expression by controlling the levels of TFs that are associated with chromatin. This can occur directly, by influencing DNA-binding ability or promoting clearance of TFs from chromatin, or indirectly, by regulating TFs abundance or localization (18).…”

Section: Sumoylation and Gene Transcriptionmentioning

confidence: 99%