SUMOylation is required for fungal development and pathogenicity in the rice blast fungus <i>Magnaporthe oryzae</i>

Lim, You-Jin; Kim, Ki-Tae; Lee, Yong-Hwan

doi:10.1111/mpp.12687

Cited by 25 publications

(35 citation statements)

References 80 publications

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“…Thirdly, a 3.8-kb gene replacement fragment were amplified with a primer set CgMCK1_F3/R3 and purified and transformed into the protoplasts of wild type C. gloeosporioides SMCG1#C as described [ 18 ]. The deletion mutant of CgMCK1 was confirmed by Southern blot analysis using a method previously described [ 21 ] with the hybridization probes of CgMCK1 and HPH , respectively.…”

Section: Methodsmentioning

confidence: 99%

The MAPKKK CgMck1 Is Required for Cell Wall Integrity, Appressorium Development, and Pathogenicity in Colletotrichum gloeosporioides

Fang

Xia

Wang

et al. 2018

Genes

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Mitogen-activated protein kinase (MAPK) signaling pathway plays key roles in sensing extracellular signals and transmitting them from the cell membrane to the nucleus in response to various environmental stimuli. A MAPKKK protein CgMck1 in Colletotrichum gloeosporioides was characterized. Phenotypic analyses of the ∆Cgmck1 mutant showed that the CgMck1 was required for vegetative growth, fruiting body development, and sporulation. Additionally, the CgMCK1 deletion mutant showed significant defects in cell wall integrity, and responses to osmotic stresses. The mutant abolished the ability to develop appressorium, and lost pathogenicity to host plants. The ∆Cgmck1 mutant also exhibited a higher sensitivity to antifungal bacterium agent Bacillus velezensis. The deletion mutants of downstream MAPK cascades components CgMkk1 and CgMps1 showed similar defects to the ∆Cgmck1 mutant. In conclusion, CgMck1 is involved in the regulation of vegetative growth, asexual development, cell wall integrity, stresses resistance, and infection morphogenesis in C. gloeosporioides.

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Section: Methodsmentioning

confidence: 99%

The MAPKKK CgMck1 Is Required for Cell Wall Integrity, Appressorium Development, and Pathogenicity in Colletotrichum gloeosporioides

Fang

Xia

Wang

et al. 2018

Genes

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“…In this study, some of these types of genes or protein products were clearly downregulated or misregulated in the ΔVdCmr1 strain. Among the various post-translational modifications, SUMOylation is a conserved process of attachment of a SUMO to a protein substrate in eukaryotes (Lim et al, 2018). This process regulates many important biological mechanisms, including transcriptional regulation, protein stabilization, cell cycle, DNA repair, and pathogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…This process regulates many important biological mechanisms, including transcriptional regulation, protein stabilization, cell cycle, DNA repair, and pathogenesis. Sumoylation is also involved in responding to different stresses (Liu et al, 2018) and is required for infection-related fungal development, stress responses and pathogenicity in Magnaporthe oryzae (Lim et al, 2018). Ubiquitination plays an important role in DNA damage repair, including regulation of cell cycle checkpoint monitoring, DNA repair and ensuing programmed death if the repair is unsuccessful (Takata et al, 1998;Abraham 2001;Huen and Chen, 2008).…”

Section: Discussionmentioning

confidence: 99%

Insights into VdCmr1‐mediated protection against high temperature stress and UV irradiation in Verticillium dahliae

Fang

Klosterman

Tian

et al. 2019

Environmental Microbiology

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Summary The fungus Verticillium dahliae causes vascular wilt disease on more than 200 plant species worldwide. This fungus can survive for years in soil as melanized microsclerotia. We found that VdCmr1, a transcription factor, is required for the melanin production and increased survival following UV irradiation in V. dahliae but not for microsclerotia production or virulence. Here, we provided evidence how VdCmr1 protects against high temperature (HT) and UV irradiation in V. dahliae. The results indicate that VdCmr1 mediates entry to the diapause period in V. dahliae in response to HT and contributes to the expression of proteins to minimize protein misfolding and denaturation. VdCmr1 deletion results in the misregulation of DNA repair machinery, suggestive of reduced DNA repair capacity following UV irradiation and in correlation with the low survival rate of UV‐treated VdCmr1 mutants. We discovered a putative VdCmr1‐dependent gene cluster associated with secondary metabolism and stress responses. We also functionally characterized two VdCmr1‐responsive genes participating in HT and UV response. These results shed further light on the roles of VdCmr1 in protection from HT or UV irradiation, and the additional insights into the mechanisms of this protection may be useful to exploit for more effective disease control.

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“…Generally, deficiencies in penetration and infectious growth are the two main limiting factors for the impaired virulence of pathogens. In most researches for M. oryzae , the penetration ratio in the first 24 hours post inoculation (hpi) is often taken as an indicator of the ability to penetrate [28,29]. Consequently, an inoculation assay using rice sheaths was conducted to observe the infection process under a microscope.…”

Section: Resultsmentioning

confidence: 99%

The Secreted Protein MoHrip1 Is Necessary for the Virulence of Magnaporthe oryzae

Nie

Zhang

ZHUANG

et al. 2019

IJMS

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Secreted effectors from Magnaporthe oryzae play critical roles in the interaction with rice to facilitate fungal infection and disease development. M. oryzae-secreted protein MoHrip1 can improve plant defense as an elicitor in vitro, however, its biological function in fungal infection is not clear. In this study, we found that the expression of mohrip1 was significantly induced in the stages of fungal penetration and colonization. Although dispensable for the growth and conidiation, MoHrip1 was necessary for the full virulence of M. oryzae. Deletion of mohrip1 remarkably compromised fungal virulence on rice seedlings and even on rice leaves with wounds. Rice sheath inoculation assay further demonstrated the defects of mohrip1-deleted mutants on penetration and proliferation in rice cells. Additionally, compared with WT and complementation strain, the inoculation of mohrip1-deleted mutants induced a higher expression of specific defense related genes and a higher production of specific defensive compounds in rice leaves. These data collectively indicated that MoHrip1 is necessary for fungal penetration and invasive expansion, and further full virulence of rice blast fungus.

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SUMOylation is required for fungal development and pathogenicity in the rice blast fungus Magnaporthe oryzae

Cited by 25 publications

References 80 publications

The MAPKKK CgMck1 Is Required for Cell Wall Integrity, Appressorium Development, and Pathogenicity in Colletotrichum gloeosporioides

The MAPKKK CgMck1 Is Required for Cell Wall Integrity, Appressorium Development, and Pathogenicity in Colletotrichum gloeosporioides

Insights into VdCmr1‐mediated protection against high temperature stress and UV irradiation in Verticillium dahliae

The Secreted Protein MoHrip1 Is Necessary for the Virulence of Magnaporthe oryzae

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