Regulation of the Peroxidase‐Like Activity of nGO, MoS₂ and WS₂ Nanozymes by Using Metal Cations

Abstract

Two dimensional nanoparticles (2D‐NPs) along with other nanoscale materials have been deemed to be the next generation of artificial enzymes (nanozymes). The low‐cost bulk‐scale production, ease of storage and modification of such nanomaterials have given nanozymes an advantage over traditional enzymes. Many studies have been aimed at developing methods to increase the performance of these nanozymes, and also identify interfering agents. To investigate the interference of a number of metal cations, we studied the effect of Ti2+, Fe2+, Ag+, Hg2+, Co2+, Cu2+, Ni2+, Pb2+, Ca2+, Zn2+ and Mn2+ in a nanozyme assays of 2D‐NPs using ABTS radical formation. Ti2+, Co2+, Cu2+, Ni2+, Ca2+, Zn2+ and Mn2+ ions did not display any notable effect on the peroxidase‐like activity of nGO, MoS2 and WS2 2D‐NPs. However, Fe2+, Ag+, Hg2+ and Pb2+ ions’ effects on the overall ABTS reaction were significant enough to be visualised by partial least square discriminant analysis (PLSDA). We report that, similar to that of many natural enzymes, the nanozyme activity of 2D‐NPs is regulated by a number of metal cations allowing their identification and discrimination by using a statistical analysis tool.

Reactive oxygen nanobiocatalysts: activity-mechanism disclosures, catalytic center evolutions, and changing states

“…206 This section will give a brief discussion of each type of emerged 2D material to act as RONBCs and the corresponding modification strategies. For the TMCs, the iron sulfide (FeS), [207][208][209][210] zinc sulfide (ZnS), 211 SnS, 212 molybdenum disulfide (MoS 2 ), 90,213,214 tungsten disulfide (WS 2 ), 215 vanadium(IV) disulfide (VS 2 ), 216 and platinum disulfide (PtS 2 ) 211 have been reported with enzymelike activities (Fig. 14A(a)-(d)), among which MoS 2 nanosheets get increasing attention due to their special mechanical, optical, electrical structure, ultrathin atomic layer structure, low toxicity, and good biocompatibility.…”

Transition Metal Dichalcogenides (TMDC)-Based Nanozymes for Biosensing and Therapeutic Applications

“…Chitosan modified MoSe 2 showed optimal POD activity at pH 3.5 and 55 °C temperature; higher pH or lower temperatures were linked with lower activity [ 28 ]. The presence of Fe 2+ ion increased POD activity of WS 2 NZs [ 62 ], whereas the presence of Pb 2+ ion hindered it [ 63 ].…”

Quantification of Neuronal Cell-Released Hydrogen Peroxide Using 3D Mesoporous Copper-Enriched Prussian Blue Microcubes Nanozymes: A Colorimetric Approach in Real Time and Anticancer Effect

“…Magnetite nanoparticles (Fe 3 O 4 NPs) were among the first reported nanozymes to possess peroxidase-like (POD) activity alike horseradish peroxidase (HRP) . Since then, an array of nanostructures, including monometallic nanomaterials like Au NPs, Ag NPs, Pt NPs, and bimetallic nanoparticles such as Au@Ag nanorods, porous PtCu dendrites, Pt 74 Ag 26 , FePt–Au, metal oxides like MoS 2 , WS 2 , Co 3 O 4 , cerium oxide, as well as mixed metal oxides like Cr 2 O 2 –TiO 2 , ZnCo 2 O 4 , Fe 3 O 4 –Fe 0 /Fe 3 C, metal cluster nanomaterials like mercaptosuccinic acid (MSA)-stabilized Cu nanoclusters, and Fe–N/C single-atom nanozymes, along with metal–organic framework-based nanomaterials, , have found wide applications in colorimetric biosensors for detecting H 2 O 2 . However, these nanostructured materials exhibit certain drawbacks, such as complex synthesis processes and limited biocompatibility.…”