Regulation of Srpr Expression by miR-330-5p Controls Proliferation of Mouse Epidermal Keratinocyte

Kim, Bong-Kyu; Yoo, Hye-In; Choi, Kyu-Yong; Lee, Ahreum; Yoon, Sungjoo Kim

doi:10.1371/journal.pone.0164896

Cited by 12 publications

(11 citation statements)

References 21 publications

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“…Because our previously miRNAs array also identified significant differential expression of miR-330-5p in the skin of different coat colors of animals [18,19]. Other report indicated that keratinocytes produce miR-330-5p [27] and miR-330-5p downregulated the expression of TYR that plays a key role in pigmentation [28,29]. Whether and how miR-330-5p regulates TYR in melanocytes to regulate pigmentation is not clear.…”

Section: Introductionmentioning

confidence: 94%

Exosomal miRNA derived from keratinocytes regulates pigmentation in melanocytes

Liu

Gao

et al. 2019

Journal of Dermatological Science

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Background: Pigmentation is controlled by complex mechanisms. Evidence suggests that miRNAs can regulate pigmentation. However, the mechanism has not been fully elucidated. Objective In this study, we revealed a novel mechanism that regulates pigmentation involving exosomes, miRNAs and the crosstalk between keratinocytes and melanocytes. Methods: The expression and localization of exosome specific marker TSG101 in keratinocytes and melanocytes; Changes of melanin content in melanocytes after co-culture of exosome and melanocytes; Expression changes of target gene TYR and its related genes and inhibitory effect of miR-330-5p on pigmentation were studied by using various molecular biological techniques. Results: In this experiment, we used miR-330-5p in keratinocytes to verify the effect of keratinocyte derived exosome on melanocyte pigmentation. First, we found that keratinocytes secrete exosomes carrying miR-330-5p; moreover, greater miR-330-5p expression was found in exosomes derived from keratinocytes that overexpressed miR-330-5p. Second, we found that exosomes derived from keratinocytes with overexpression of miR-330-5p caused a significant increase in miR-330-5p in melanocytes. Finally, exosomes derived from keratinocytes that overexpressed miR-330-5p induced a significant decrease in the production of melanin and expression of TYR in melanocytes. Meanwhile, we overexpressed miR-330-5p in melanocytes, which also proved the inhibitory effect of miR-330-5p on pigmentation. Conclusion: These findings suggest that keratinocytes crosstalk with melanocytes in the epidermal melanin unit via exosomal miRNAs. These studies reveal an important role of exosomes in melanocyte pigmentation, which opens a new pathway of melanogenesis.

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Section: Introductionmentioning

confidence: 94%

Exosomal miRNA derived from keratinocytes regulates pigmentation in melanocytes

Liu

Gao

et al. 2019

Journal of Dermatological Science

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“…RNA-binding proteins have been shown to be the key units to regulating RNA metabolism (Fu & Ares, 2014;Stefanie, Markus & Thomas, 2014;Moore & Proudfoot, 2009) and dynamically interact with both coding and noncoding RNA (Kim et al, 2016). Furthermore, recent studies have shown that RBPs are down-regulated in cancers (Wang et al, 2018), but the study of 16 tissues from 80 healthy individuals indicated that RBPs show the higher expression than non-RBPs (Bobak & Sarath, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…TRNAU1AP has been confirmed to play an important role in the regulation of cell proliferation and migration via the PI3K/Akt signaling pathway (Hu et al, 2018). SRPR is reported to regulate keratinocyte proliferation by affecting cell cycle progression and tend to show high expression in epidermal keratinocytes (Kim et al, 2016). RBMS3 has been found to inhibit breast cancer cell proliferation and tumorigenesis by inactivating the Wnt/β-catenin signaling pathway (Yang, Quan & Ling, 2018).…”

Section: Introductionmentioning

confidence: 99%

Peer Review #1 of "The co-expression networks of differentially expressed RBPs with TFs and LncRNAs related to clinical TNM stages of cancers (v0.1)"

Corleone¹

2019

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Background: RNA-binding proteins (RBPs) play important roles in cellular homeostasis by regulating the expression of thousands of transcripts, which have been reported to be involved in human tumorigenesis. Despite previous reports of the dysregulation of RBPs in cancers, the degree of dysregulation of RBPs in cancers and the intrinsic relevance between dysregulated RBPs and clinical TNM information remains unknown. Furthermore, the co-expressed networks of dysregulated RBPs with transcriptional factors and lncRNAs also require further investigation. Results: Here, we firstly analyzed the deviations of expression levels of 1,542 RBPs from 20 cancer types and found that (1) RBPs are dysregulated in almost all 20 cancer types, especially in BLCA, COAD, READ, STAD, LUAD, LUSC and GBM with proportion of deviation larger than 300% compared with non-RBPs in normal tissues. (2) Up-and down-regulated RBPs also show opposed patterns of differential expression in cancers and normal tissues. In addition, down-regulated RBPs show a greater degree of dysregulated expression than up-regulated RBPs do. Secondly, we analyzed the intrinsic relevance between dysregulated RBPs and clinical TNM information and found that (3) Clinical TNM information for two cancer types-CHOL and KICH-is shown to be closely related to patterns of differentially expressed RBPs (DE RBPs) by co-expression cluster analysis. Thirdly, we identified ten key RBPs (seven downregulated and three up-regulated) in CHOL and seven key RBPs (five down-regulated and two up-regulated) in KICH by analyzing co-expression correlation networks. Fourthly, we constructed the co-expression networks of key RBPs between 1,570 TFs and 4,147 lncRNAs for CHOL and KICH, respectively. Conclusions: These results may provide an insight into the understanding of the functions of RBPs in human carcinogenesis. Furthermore, key RBPs and the co-expressed networks offer useful information for potential prognostic biomarkers and therapeutic targets for patients with cancers at the N and M stages in two cancer types CHOL and KICH.

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“…In humans, downregulation of miR‐330‐5p expression may affect colorectal cancer development via modulation of integrin α5 (ITGA5) expression (Yoo, Kim, & Yoon, ). MiR‐330‐5p‐mediated regulation of SRPR is needed for the regulation of proliferation of epidermal keratinocytes in mice (Kim, Yoo, Choi, Lee, & Yoon, ). The role of miR‐330‐5p in cattle, pigs and other livestock requires further study.…”

Section: Discussionmentioning

confidence: 99%

MiR‐330‐5p negatively regulates ovine preadipocyte differentiation by targeting branched‐chain aminotransferase 2

Shi

Yan

Qiao

et al. 2018

Animal Science Journal

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The differentiation of preadipocytes into adipose tissues is tightly regulated by various factors including microRNAs and cytokines. This article aims to study the effect of miR-330-5p on expression of BCAT2 in ovine preadipocytes. Ovine preadipocytes were isolated, and we found that the miR-330-5p expression decreased gradually during the early differentiation of ovine preadipocytes, while BCAT2 expression increased. BCAT2 was identified as a direct target of miR-330-5p, ectopic expression of miR-330-5p could change the expression of both BCAT2 mRNA and protein. Silencing BCAT2 had the same inhibition effects as overexpressing miR-330-5p on the preadipocyte differentiation, but overexpressing BCAT2 had the converse effects. Taken together, we demonstrated that miR-330-5p is a negative regulator of differentiation by targeting BCAT2, and clarified the role of BCAT2 and miR-330-5p during preadipocyte differentiation.

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Regulation of Srpr Expression by miR-330-5p Controls Proliferation of Mouse Epidermal Keratinocyte

Cited by 12 publications

References 21 publications

Exosomal miRNA derived from keratinocytes regulates pigmentation in melanocytes

Exosomal miRNA derived from keratinocytes regulates pigmentation in melanocytes

Peer Review #1 of "The co-expression networks of differentially expressed RBPs with TFs and LncRNAs related to clinical TNM stages of cancers (v0.1)"

MiR‐330‐5p negatively regulates ovine preadipocyte differentiation by targeting branched‐chain aminotransferase 2

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