MiR‐330‐5p negatively regulates ovine preadipocyte differentiation by targeting branched‐chain aminotransferase 2

Shi, Tao; Yan, Xin; Qiao, Liying; Li, Baojun; Liu, Cheng; Jing, Jiongjie; Cao, Ningxian; Liu, Wenzhong

doi:10.1111/asj.12995

Cited by 8 publications

(6 citation statements)

References 34 publications

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“…For example, miR-330-5p can regulate macrophage M2 polarization and insulin resistance in diabetic mice (Sun et al, 2018). MiR-330-5p can negatively regulate ovine preadipocyte differentiation by targeting branched-chain aminotransferase 2 (Shi et al, 2018). In addition, miR-330-5p can suppress glioblastoma cell proliferation and invasion through targeting ITGA5 (Feng, Ma, Ji, Liu, & Hu, 2017).…”

Section: Discussionmentioning

confidence: 99%

Silence of long intergenic noncoding RNA HOTAIR ameliorates oxidative stress and inflammation response in ox‐LDL‐treated human macrophages by upregulating miR‐330‐5p

Liu

Huang

2018

Journal Cellular Physiology

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Evidence of the involvement of long noncoding RNAs (lncRNAs) in atherosclerosis is growing but still not well characterized. Here, we concentrated on the biological roles of lncRNA HOX transcription antisense RNA (HOTAIR) in atherosclerosis. In our study, we found that oxidized low-density lipoprotein (ox-LDL) induced human macrophages THP-1 cells apoptosis dose dependently and time dependently. Meanwhile, HOTAIR was significantly increased in THP-1 cells treated with ox-LDL. Then, HOTAIR was modulated by infection of LV-short hairpin RNA (shRNA) and LV-HOTAIR into THP-1 cells. As displayed, CD36, Oil Red O staining levels, total cholesterol, triglyceride levels and dil-ox-LDL uptake rate were greatly repressed by the silence of HOTAIR while triggered by overexpression of HOTAIR. Moreover, knockdown of HOTAIR suppressed reactive oxygen species, malondialdehyde levels, increased superoxide dismutase activity and cell apoptosis were also restrained. Reversely, overexpression of HOTAIR exhibited an opposite phenomenon. In addition, interleukin 6 (IL-6), IL-1β, cyclo-oxygenase 2, and tumor necrosis factor α protein levels were significantly depressed by LV-shRNA) of HOTAIR while increased by upregulation of HOTAIR in THP-1 cells. By carrying out bioinformatics analysis, miR-330-5p was predicted as a target of HOTAIR and the correlation between them was validated in our current study. MiR-330-5p was greatly decreased in THP-1 cells incubated with ox-LDL and overexpression of miR-330-5p was able to inhibit oxidative stress and inflammation process. Taken together, it was implied that HOTAIR contributed to atherosclerosis development by downregulating miR-330-5p in human macrophages.

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Section: Discussionmentioning

confidence: 99%

Silence of long intergenic noncoding RNA HOTAIR ameliorates oxidative stress and inflammation response in ox‐LDL‐treated human macrophages by upregulating miR‐330‐5p

Liu

Huang

2018

Journal Cellular Physiology

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“…BCAT2 expression is downregulated by miR-182 ( Wang et al, 2017 ). miR-330-5p is another negative regulator of BCAT2, as there is an inverse relationship between the two during ovine preadipocyte differentiation ( Shi et al, 2018 ). miR-125a-3p is involved in AA and glucose metabolism, and can inhibit BCAA metabolism by negatively regulating KLF-15, an upstream regulator of BCAT2 in skeletal muscle of fish ( Li H. et al, 2020 ).…”

Section: Bcat2 and Its Regulation In Skeletal Musclementioning

confidence: 99%

Branched-chain Amino Acids: Catabolism in Skeletal Muscle and Implications for Muscle and Whole-body Metabolism

et al. 2021

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Branched-chain amino acids (BCAAs) are critical for skeletal muscle and whole-body anabolism and energy homeostasis. They also serve as signaling molecules, for example, being able to activate mammalian/mechanistic target of rapamycin complex 1 (mTORC1). This has implication for macronutrient metabolism. However, elevated circulating levels of BCAAs and of their ketoacids as well as impaired catabolism of these amino acids (AAs) are implicated in the development of insulin resistance and its sequelae, including type 2 diabetes, cardiovascular disease, and of some cancers, although other studies indicate supplements of these AAs may help in the management of some chronic diseases. Here, we first reviewed the catabolism of these AAs especially in skeletal muscle as this tissue contributes the most to whole body disposal of the BCAA. We then reviewed emerging mechanisms of control of enzymes involved in regulating BCAA catabolism. Such mechanisms include regulation of their abundance by microRNA and by post translational modifications such as phosphorylation, acetylation, and ubiquitination. We also reviewed implications of impaired metabolism of BCAA for muscle and whole-body metabolism. We comment on outstanding questions in the regulation of catabolism of these AAs, including regulation of the abundance and post-transcriptional/post-translational modification of enzymes that regulate BCAA catabolism, as well the impact of circadian rhythm, age and mTORC1 on these enzymes. Answers to such questions may facilitate emergence of treatment/management options that can help patients suffering from chronic diseases linked to impaired metabolism of the BCAAs.

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“…In recent years, many studies have found that miRNAs are involved in many biological processes, such as disease and metabolism [9,10]. Some studies have also shown that miRNAs play an important role in regulating adipocyte differentiation and lipid metabolism [11,12]. KLF13 targeted by miR-425-5p inhibits adipocyte differentiation during porcine intramuscular adipocyte differentiation [13] and miR-26a promoting 3T3-L1 adipocyte differentiation via targeting regulatory PTEN [14].…”

Section: Introductionmentioning

confidence: 99%

MiRNAs and mRNAs Analysis during Abdominal Preadipocyte Differentiation in Chickens

Sun

Zhu

et al. 2020

Animals

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The excessive deposition of abdominal fat has become an important factor in restricting the production efficiency of chickens, so reducing abdominal fat deposition is important for improving growth rate. It has been proven that miRNAs play an important role in regulating many physiological processes of organisms. In this study, we constructed a model of adipogenesis by isolating preadipocytes (Ab-Pre) derived from abdominal adipose tissue and differentiated adipocytes (Ab-Ad) in vitro. Deep sequencing of miRNAs and mRNAs expressed in Ab-Pre and Ab-Ad groups was conducted to explore the effect of miRNAs and mRNAs on fat deposition. We identified 80 differentially expressed miRNAs (DEMs) candidates, 58 of which were up-regulated and 22 down-regulated. Furthermore, six miRNAs and six mRNAs were verified by qRT-PCR, and the results showed that the expression of the DEMs and differentially expressed genes (DEGs) in the two groups was consistent with our sequencing results. When target genes of miRNA were combined with mRNA transcriptome data, a total of 891 intersection genes were obtained, we predicted the signal pathways of cross genes enrichment to the MAPK signal pathway, insulin signal pathway, fatty acid metabolism, and ECM–receptor interaction. Meanwhile, we constructed miRNA and negatively correlated mRNA target networks, including 12 miRNA–mRNAs pairs, which showed a strong association with the abdominal adipocyte differentiation (miR-214−ACSBG2, NFKB2, CAMK2A, ACLY, CCND3, PLK3, ITGB2; miR-148a-5p−ROCK2; miR-10a-5p−ELOVL5; miR-146b-5p−LAMA4; miR-6615-5p−FLNB; miR-1774−COL6A1). Overall, these findings provide a background for further research on lipid metabolism. Thus, we can better understand the molecular genetic mechanism of chicken abdominal fat deposition.

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MiR‐330‐5p negatively regulates ovine preadipocyte differentiation by targeting branched‐chain aminotransferase 2

Cited by 8 publications

References 34 publications

Silence of long intergenic noncoding RNA HOTAIR ameliorates oxidative stress and inflammation response in ox‐LDL‐treated human macrophages by upregulating miR‐330‐5p

Silence of long intergenic noncoding RNA HOTAIR ameliorates oxidative stress and inflammation response in ox‐LDL‐treated human macrophages by upregulating miR‐330‐5p

Branched-chain Amino Acids: Catabolism in Skeletal Muscle and Implications for Muscle and Whole-body Metabolism

MiRNAs and mRNAs Analysis during Abdominal Preadipocyte Differentiation in Chickens

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