Regulation of spatiotemporal expression of cell–cell adhesion molecules during development of <i>Dictyostelium discoideum</i>

Siu, Chi‐Hung; Sriskanthadevan, Shrivani; Wang, Jun; Liansheng, Hou; Chen, Gong; Xu, Xiaoqun; Thomson, Alexander; Yang, Chunxia

doi:10.1111/j.1440-169x.2011.01267.x

Cited by 26 publications

(30 citation statements)

References 70 publications

(85 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Intercellular signaling presumably becomes more efficient and important as cells begin to adhere to each other during the aggregation stage of development. The onset of cell-cell adhesion occurs as cells become responsive to cAMP and this adhesion allows cells form streams as they aggregate into mounds [46]. Chemotactic responses to folate do not facilitate cell-cell adhesion but groups of cells can chemotax to folate while in contact with each other (unpublished results).…”

Section: Discussionmentioning

confidence: 99%

The Dictyostelium MAPK ERK1 is phosphorylated in a secondary response to early developmental signaling

Schwebs¹,

Hadwiger²

2015

Cellular Signalling

View full text Add to dashboard Cite

Previous reports have suggested that the two mitogen-activated protein kinases (MAPKs) in Dictyostelium discoideum, ERK1 and ERK2, can be directly activated in response to external cAMP even though these MAPKs play different roles in the developmental life cycle. To better characterize MAPK regulation, the levels of phosphorylated MAPKs were analyzed in response to external signals. Only ERK2 was rapidly phosphorylated in response to the chemoattractants, cAMP and folate. In contrast, the phosphorylation of ERK1 occurred as a secondary or indirect response to these stimuli and this phosphorylation was enhanced by cell-cell interactions, suggesting that other external signals can activate ERK1. The phosphorylation of ERK1 or ERK2 did not require the function of the other MAPK in these responses. Folate stimulation of a chimeric population of erk1− and gα4− cells revealed that the phosphorylation of ERK1 could be mediated through an intercellular signal other than folate. Loss of ERK1 function suppressed the developmental delay and the deficiency in anterior cell localization associated with gα5− mutants suggesting that ERK1 function can be down regulated through Gα5 subunit-mediated signaling. However, no major changes in the phosphorylation of ERK1 were observed in gα5− cells suggesting that the Gα5 subunit signaling pathway does not regulate the phosphorylation of ERK1. These findings suggest that the activation of ERK1 occurs as a secondary response to chemoattractants and that other cell-cell signaling mechanisms contribute to this activation. Gα5 subunit signaling can down regulate ERK1 function to promote prestalk cell development but not through major changes to the level of phosphorylated ERK1.

show abstract

Section: Discussionmentioning

confidence: 99%

The Dictyostelium MAPK ERK1 is phosphorylated in a secondary response to early developmental signaling

Schwebs¹,

Hadwiger²

2015

Cellular Signalling

View full text Add to dashboard Cite

show abstract

“…The origin of multicellularity is closely associated with cell-cell adhesion molecules (CAMs), and we analyzed the expression of this group of genes in detail [21]. There are four adhesion systems in D. discoideum , three of which are well-characterized [22]. EDTA-sensitive, Ca 2+ -dependent adhesion sites are mediated by the protein DdCAD-1, which is encoded by the gene cadA , while the two EDTA-insensitive systems include the gp80 protein, encoded by csaA, and gp150 encoded by tgrC1 [23–26].…”

Section: Resultsmentioning

confidence: 99%

Sociogenomics of self vs. non-self cooperation during development of Dictyostelium discoideum

Buttery

Thompson

et al. 2014

BMC Genomics

View full text Add to dashboard Cite

BackgroundDictyostelium discoideum, a microbial model for social evolution, is known to distinguish self from non-self and show genotype-dependent behavior during chimeric development. Aside from a small number of cell-cell recognition genes, however, little is known about the genetic basis of self/non-self recognition in this species. Based on the key hypothesis that there should be differential expression of genes if D. discoideum cells were interacting with non-clone mates, we performed transcriptomic profiling study in this species during clonal vs. chimeric development. The transcriptomic profiles of D. discoideum cells in clones vs. different chimeras were compared at five different developmental stages using a customized microarray. Effects of chimerism on global transcriptional patterns associated with social interactions were observed.ResultsWe find 1,759 genes significantly different between chimera and clone, 1,144 genes associated significant strain differences, and 6,586 genes developmentally regulated over time. Principal component analysis showed a small amount of the transcriptional variance to chimerism-related factors (Chimerism: 0.18%, Chimerism × Timepoint: 0.03%). There are 162 genes specifically regulated under chimeric development, with continuous small differences between chimera vs. clone over development. Almost 60% of chimera-associated differential genes were differentially expressed at the 4 h aggregate stage, which corresponds to the initial transition of D. discoideum from solitary life to a multicellular phase.ConclusionsA relatively small proportion of over-all variation in gene expression is explained by differences between chimeric and clonal development. The relatively small modifications in gene expression associated with chimerism is compatible with the high level of cooperation observed among different strains of D. discoideum; cells of distinct genetic backgrounds will co-aggregate indiscriminately and co-develop into fruiting bodies. Chimeric development may involve re-programming of the transcriptome through small modifications of the developmental genetic network, which may also indicate that response to social interaction involves many genes with individually small transcriptional effect.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-616) contains supplementary material, which is available to authorized users.

show abstract

“…This suggests that Ca 2+ -dependent cell–cell interaction facilitates the formation of the clusters. In D. discoideum , CadA (Gp24) is the major cell adhesion molecule mediating Ca 2+ -dependent cell–cell interaction (Siu et al, 2011). Therefore, we tested whether anti-CadA serum blocks the formation of the clusters.…”

Section: Resultsmentioning

confidence: 99%

The G alpha subunit Gα8 inhibits proliferation, promotes adhesion and regulates cell differentiation

Wu¹,

Janetopoulos²

2013

Developmental Biology

View full text Add to dashboard Cite

Heterotrimeric G protein-mediated signal transduction plays a pivotal role in both vegetative and developmental stages in the eukaryote Dictyostelium discoideum. Here we describe novel functions of the G protein alpha subunit Gα8 during vegetative and development stages. Gα8 is expressed at low levels during vegetative growth. Loss of Gα8 promotes cell proliferation, whereas excess Gα8 expression dramatically inhibits growth and induces aberrant cytokinesis on substrates in a Gβ-dependent manner. Overexpression of Gα8 also leads to increased cell–cell cohesion and cell–substrate adhesion. We demonstrate that the increased cell–cell cohesion is mainly caused by induced CadA expression, and the induced cell– substrate adhesion is responsible for the cytokinesis defects. However, the expression of several putative constitutively active mutants of Gα8 does not augment the phenotypes caused by intact Gα8. Gα8 is strongly induced after starvation, and loss of Gα8 results in decreased expression of certain adhesion molecules including CsA and tgrC1. Interestingly, Gα8 is preferentially distributed in the upper and lower cup of the fruiting body. Lack of Gα8 decreases the expression of the specific marker of the anterior-like cells, suggesting that Gα8 is required for anterior-like cell differentiation.

show abstract

Regulation of spatiotemporal expression of cell–cell adhesion molecules during development of Dictyostelium discoideum

Cited by 26 publications

References 70 publications

The Dictyostelium MAPK ERK1 is phosphorylated in a secondary response to early developmental signaling

The Dictyostelium MAPK ERK1 is phosphorylated in a secondary response to early developmental signaling

Sociogenomics of self vs. non-self cooperation during development of Dictyostelium discoideum

The G alpha subunit Gα8 inhibits proliferation, promotes adhesion and regulates cell differentiation

Contact Info

Product

Resources

About