Regulation of soluble CD127 protein release and corresponding transcripts expression in T lymphocytes from septic shock patients

Mouillaux, Julie; Allam, Camille; Rimmelé, Thomas; Uberti, Thomas; Delwarde, Benjamin; Textoris, Julien; Monneret, Guillaume; Peronnet, Estelle; Venet, Fabienne

doi:10.1186/s40635-018-0220-3

Cited by 3 publications

(3 citation statements)

References 12 publications

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“…2B). Together, these data demonstrate the occurrence of spontaneous release of the soluble receptor, consistent with previous observations [15,33].…”

Section: Resultssupporting

confidence: 92%

IL‐7 induces sCD127 release and mCD127 downregulation in human CD8⁺ T cells by distinct yet overlapping mechanisms, both of which are impaired in HIV infection

et al. 2020

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The IL-7 receptor specific α chain, CD127, can be expressed both as a membraneassociated (mCD127) and a soluble form (sCD127), however, the mechanisms involved in their regulation remain to be defined. We first demonstrated in primary human CD8 + T cells that IL-7-induced downregulation of mCD127 expression is dependent on JAK and PI3K signaling, whereas IL-7-induced sCD127 release is also mediated by STAT5. Following stimulation with IL-7, expression of alternatively spliced variants of the CD127 gene, sCD127 mRNA, is reduced, but to a lesser degree than the full-length gene. Evaluation of the role of proteases revealed that MMP-9 was involved in sCD127 release, without affecting the expression of mCD127, suggesting it does not induce direct shedding from the cell surface. Since defects in the IL-7/CD127 pathway occur in various diseases, including HIV, we evaluated CD8 + T cells derived from HAART-treated HIV-infected individuals and found that IL-7-induced (1) downregulation of mCD127, (2) release of sCD127, and (3) expression of the sCD127 mRNA were all impaired. Expression of mCD127 and sCD127 is, therefore, regulated by distinct, but overlapping, mechanisms and their impairment in HIV infection contributes to our understanding of the CD8 + T cell dysfunction that persists despite effective antiretroviral therapy.

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“…2B). Together, these data demonstrate the occurrence of spontaneous release of the soluble receptor, consistent with previous observations [15,33].…”

Section: Resultssupporting

confidence: 92%

IL‐7 induces sCD127 release and mCD127 downregulation in human CD8⁺ T cells by distinct yet overlapping mechanisms, both of which are impaired in HIV infection

et al. 2020

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“…140 In the setting of septic shock, sCD127 levels appear to be altered. Results, however, seem conflicting as sCD127 levels have been shown to be upregulated 141 or downregulated 142 when compared to healthy controls. Additionally, Delwarde et al observed lower levels of mRNA encoding total CD127, only mCD127 or only sCD127 in patients with septic shock.…”

Section: Role Of Scd127 In Disease Pathogenesismentioning

confidence: 98%

Soluble IL-7Rα/sCD127 in Health, Disease, and Its Potential Role as a Therapeutic Agent

Barros¹,

Berthoud²,

Aloufi³

et al. 2021

ITT

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Soluble cytokine receptors can influence immune responses by modulating the biological functions of their respective ligands. These effects can be either agonistic or antagonistic and a number of soluble cytokine receptors have been shown to play critical roles in both maintenance of health and disease pathogenesis. Soluble IL-7Ra (sCD127) is one such example. With its impact on the IL-7/CD127 pathway, which is fundamental for the development and homeostasis of T cells, the role of sCD127 in health and disease has been extensively studied in recent years. Within this review, the role of sCD127 in maintaining host immune function is presented. Next, by addressing genetic factors affecting sCD127 expression and the associated levels of sCD127 production, the roles of sCD127 in autoimmune disease, infections and cancer are described. Finally, advances in the field of soluble cytokine therapy and the potential for sCD127 as a biomarker and therapeutic agent are discussed.

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“…its level was significantly higher than that in healthy controls (25). Based on the above results, it can be speculated that the expression and production of sIL-7Rα may be proportional to the extent of inflammation.…”

Section: Discussionmentioning

confidence: 70%

Serum soluble interleukin-7 receptor alpha levels are negatively correlated with the simultaneous activity of antineutrophil cytoplasmic antibody-associated vasculitis

Yoon

Pyo

Ahn

et al. 2022

Clinical and Experimental Rheumatology

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ObjectivesThis study investigated whether serum soluble interleukin-7 receptor alpha (sIL-7Rα) levels could reflect the simultaneous activity of antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). were compared between patients at different time points and between patients and healthy controls. Methods Sixty patients with AAV were included in this study. AAV-related variables and clinical and laboratory data were collected at the two-time points (at early high and late low BVAS) for each patient along with blood sampling. Serum sIL-7Rα levels and the populations of CD3+CD4+ and CD3+CD8+ T cells expressing membranous ResultsSerum sIL-7Rα levels were significantly lower in AAV patients at early high BVAS than in those at late low BVAS, and the direction of change in serum sIL-7Rα levels increased as BVAS decreased. Serum sIL-7Rα levels were inversely correlated with BVAS, erythrocyte sedimentation rate and C-reactive protein levels. In addition, serum sIL-7Rα levels in AAV patients at early high BVAS exhibited significantly lower levels than those in healthy controls. Particularly, AAV patients at early high BVAS showed significantly increased populations of CD3+ T cells and CD3+CD8+ T cells expressing mIL-7Rα compared to those at late low BVAS. ConclusionThis study demonstrated that not only serum sIL-7Rα levels but also the populations of CD3+ and CD3+CD8+ T cells expressing m IL-7Rα were negatively correlated with simultaneous BVAS in patients with AAV. Therefore, we suggest that serum sIL-7Rα levels can be an additional and useful biomarker for assessing the simultaneous activity of AAV.

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Regulation of soluble CD127 protein release and corresponding transcripts expression in T lymphocytes from septic shock patients

Cited by 3 publications

References 12 publications

IL‐7 induces sCD127 release and mCD127 downregulation in human CD8⁺ T cells by distinct yet overlapping mechanisms, both of which are impaired in HIV infection

IL‐7 induces sCD127 release and mCD127 downregulation in human CD8⁺ T cells by distinct yet overlapping mechanisms, both of which are impaired in HIV infection

Soluble IL-7Rα/sCD127 in Health, Disease, and Its Potential Role as a Therapeutic Agent

Serum soluble interleukin-7 receptor alpha levels are negatively correlated with the simultaneous activity of antineutrophil cytoplasmic antibody-associated vasculitis

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Regulation of soluble CD127 protein release and corresponding transcripts expression in T lymphocytes from septic shock patients

Cited by 3 publications

References 12 publications

IL‐7 induces sCD127 release and mCD127 downregulation in human CD8+ T cells by distinct yet overlapping mechanisms, both of which are impaired in HIV infection

IL‐7 induces sCD127 release and mCD127 downregulation in human CD8+ T cells by distinct yet overlapping mechanisms, both of which are impaired in HIV infection

Soluble IL-7Rα/sCD127 in Health, Disease, and Its Potential Role as a Therapeutic Agent

Serum soluble interleukin-7 receptor alpha levels are negatively correlated with the simultaneous activity of antineutrophil cytoplasmic antibody-associated vasculitis

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IL‐7 induces sCD127 release and mCD127 downregulation in human CD8⁺ T cells by distinct yet overlapping mechanisms, both of which are impaired in HIV infection

IL‐7 induces sCD127 release and mCD127 downregulation in human CD8⁺ T cells by distinct yet overlapping mechanisms, both of which are impaired in HIV infection