Regulation of secondary metabolism in fungi

Demain, Arnold L.

doi:10.1351/pac198658020219

Cited by 68 publications

(49 citation statements)

References 36 publications

(42 reference statements)

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“…Whereas for the carbon source, fructose was relatively favorable for the growth, but the pigment production was found to be low. In the case of carbon and nitrogen source, the mycelial growth and biosynthesis of many secondary metabolites were obtained when glucose was used as a carbon source [32], peptone and yeast extract as a suitable organic nitrogen source for the mycelial growth and pigment production [23]. Goudar et al (1999) [27] reported that the carbon sources such as sucrose and glucose influenced the growth, while maltose influenced high pigment production in fungi.…”

Section: Discussionmentioning

confidence: 99%

Aspergillus Terreus Kmbf1501 a Potential Pigment Producer Under Submerged Fermentation

Akilandeswari

Pradeep

2017

Int J Pharm Pharm Sci

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Objective: The present study was aimed to identify the fungal isolate from soil and to understand the different optimized parameters better to facilitate the pigment production that has high yield and stability.Methods: Aspergillus sp. was isolated from Western Ghats soil by the conventional serial dilution technique and assessed as a potential pigment producer. Different broth medium such as potato dextrose broth (PDB), czapek-dox broth (CDB), malt extract broth (MEB), rose bengal broth (RBB), sabouraud dextrose broth (SDB), yeast malt extract broth (YEMB), pH (3-9), temperature (24, 27, 30, 33, 37 and 40 °C), carbon (lactose,glucose,sucrose, maltose, galactose and fructose) and nitrogen source (peptone, yeast extract, urea and inorganic nitrogen sources like potassium nitrate, ammonium chloride and sodium nitrate), mineral salts such as sodium dihydrogen phosphate (Na2H2Po4), magnesium sulphate (Mg2So4), calcium chloride (CaCl2), copper sulphate (Cu2So4), potassium dihydrogen phosphate (KH2Po4) and manganese sulphate (Mn2So4) and inoculum age (2-7 d) of the medium related to high pigment production were analysed.Results: Aspergillus terreus KMBF1501 was identified by ribosomal DNA sequencing showing 99% similarity with other Aspergillus terreus and the accession number (KX113516) was assigned. The optimum culture conditions for pigment production by Aspergillus terreus KMBF1501 was achieved at pH 5 (0.563±0.012 nm), temperature of 27 °C (0.382±0.001 nm) with glucose (0.501±0.002 nm) as carbon source, peptone (2.147±0.004 nm) as nitrogen source, Mg2SO4 (0.401±0.001 nm) as mineral salt and 4 d (0.324±0.001 nm) of inoculum age in PDB (0.761±0.006 nm). Conclusion:Aspergillus terreus KMBF1501 produced maximum pigment when cultured in modified PDB than in common PDB medium. The high concentration of the pigment can be used for various industrial purposes.

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Section: Discussionmentioning

confidence: 99%

Aspergillus Terreus Kmbf1501 a Potential Pigment Producer Under Submerged Fermentation

Akilandeswari

Pradeep

2017

Int J Pharm Pharm Sci

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“…The mixture was then placed in sterile 40-ml glass headspace vials covered with a polypropylene screw cap and PTFE/silicone septum (Sigma-Aldrich, St. Louis, MO). This basal medium was chosen based on preliminary studies performed in this laboratory and studies performed by Demain [28]. The corn media was autoclaved for 1 hour to avoid contamination.…”

Section: Fungal Sample Preparationmentioning

confidence: 99%

Monitoring MVOC Profiles over Time from Isolates of Aspergillus flavus Using SPME GC-MS

Sun¹,

Wood-Jones²,

Wang³

et al. 2014

JACEN

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Fungi produce a variety of microbial volatile organic compounds (MVOCs) during primary and secondary metabolism. The fungus, Aspergillus flavus, is a human, animal and plant pathogen which produces aflatoxin, one of the most carcinogenic substances known. In this study, MVOCs were analyzed using solid phase microextraction (SPME) combined with GCMS from two genetically different A. flavus strains, an aflatoxigenic strain, NRRL 3357, and a non-aflatoxigenic strain, NRRL 21882. A PDMS/CAR SPME fiber was used over 30 days to observe variations in MVOCs over time. The relative percentage of individual chemicals in several chemical classes (alcohols, aldehydes, esters, furans, hydrocarbons, ketones, and organic acids) was shown to change considerably during the varied fungal growth stages. This changing chemical profile reduces the likelihood of finding a single chemical that can be used consistently as a biomarker for fungal strain identification. In our study, discriminant analysis techniques were successfully conducted using all identified and quantified MVOCs enabling discrimination of the two A. flavus strains over the entire 30-day period. This study underscores the potential of using SPME GCMS coupled with multivariate analysis for fungi strain identification.

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“…Secondary metabolites are low-molecular weight bioactive natural products and essential for growth and survival functions of fungi [16] . Hyper saline environments originated by the evaporation of water and precipitation of the sodium chloride (NaCl) eventually increases the salinity [17] .…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Antiangiogenic and Antimetastatic Effects of Penicillium chrysogenum Secondary Metabolites

Dikmen¹

2017

ijps

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Dikmen, et al.: Antiangiogenic and Antimetastatic Effects of Penicillium chrysogenumDevelopment of resistance to standard therapy and toxic side effects are the major challenges encountered in chemotherapy of colorectal cancer. Increasing evidence has shown the ability of natural products to overcome various challenging disorders. Since, the development of antibiotics, fungi have been shown to be a wide useful source of lead compounds for the development of new pharmaceuticals. Fungal secondary metabolites from extreme environments are remarkable due to their diverse components, which makes them interesting candidates for drug discovery. In order to discover novel active antimetastatic and antiangiogenic compounds, secondary metabolites of halotolerant Penicillium chrysogenum-1 isolated from Tuz Lake in Turkey and ethyl acetate extract of halotolerant P. chrysogenum-1 was prepared from the isolate of halotolerant P. chrysogenum-1 culture medium. Firstly, antioxidant activity of the extract was determined. Then cytotoxicity and cell proliferation were investigated by WST-1 assay and real-time cell analysis system-DP on human umbilical vein endothelial cells and colorectal carcinoma cells, respectively. Antiangiogenic effects on human umbilical vein endothelial cells were evaluated with cell migration and invasion assays by using real-time cell analysis system. Also, mRNA expression levels of metastasis and angiogenesis-related genes, VEGFA, VEGFB, EGFR, COX-10, ANGPT-1 and IL-8 were determined on both cell lines. Results indicated that halotolerant P. chrysogenum-1 extract exhibited significant antimetastatic and antiangiogenic effects. According to the real-time cell analysis system results, IC 50 concentrations of halotolerant P. chrysogenum-1 extract on Caco-2 and human umbilical vein endothelial cells were calculated as 55.2 and 37.89 µg/ml, respectively for 72 h. These results indicated that the secondary metabolites of halotolerant P. chrysogenum-1 extract could have the potential as a preventive and therapeutic agent in metastatic cancer processes.

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Regulation of secondary metabolism in fungi

Abstract: Abstract

Cited by 68 publications

References 36 publications

Aspergillus Terreus Kmbf1501 a Potential Pigment Producer Under Submerged Fermentation

Aspergillus Terreus Kmbf1501 a Potential Pigment Producer Under Submerged Fermentation

Monitoring MVOC Profiles over Time from Isolates of Aspergillus flavus Using SPME GC-MS

Evaluation of Antiangiogenic and Antimetastatic Effects of Penicillium chrysogenum Secondary Metabolites

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