“…FACS staining was performed as previously described (13). Briefly, cells preincubated with the anti-FcgRII/III mAb 2.4G2 for 30 min were stained with the appropriate combinations of the following mAbs: anti-CD4, anti-TCR-b, anti-DO11.10 TCR, anti-CD45.1, anti-CD3ε, anti-IL-4, and anti-IFN-g (all from BD Pharmingen).…”