Regulation of Pyruvate Formate Lyase-Deficient Klebsiella pneumoniae for Efficient 1,3-Propanediol Bioproduction

Bao, Wenjing; Wei, Renquan; Liu, Xuxia; Dong, Shufan; Chen, Tianyu; Fu, Shuilin; Gong, H.

doi:10.1007/s00284-019-01795-5

Cited by 8 publications

(3 citation statements)

References 27 publications

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“…Using a fed-batch operation with Clostridium diolis DSM 15410, the final 1,3-PDO concentration and productivity were increased by 208% and 28% as compared to the batch culture . However, in Klebsiella pneumoniae fermentation, the fed-batch mode resulted in a 423% increase in 1,3-PDO concentration and a 248% increase in 1,3-PDO productivity over batch fermentation. , Meanwhile, a relatively high 1,3-PDO concentration (60.4 and 76.8 g/L) and productivity (2.52 and 2.56 g/L.h) were similarly achieved by Bao et al and Lin et al in fed-batch fermentation using mutant Klebsiella pneumoniae strains with gene deficiencies. However, an exception was observed in fed-batch fermentation with Clostridium perfringens GYL, where the 1,3-PDO concentration, yield, and productivity were comparable to batch fermentation .…”

Section: Smart Drop-in Biobased 13-propanediolmentioning

confidence: 98%

Palm Oil-Based Chemicals for Sustainable Development of Petrochemical Industries in Malaysia: Progress, Prospect, and Challenges

Chin

Shahruddin

Chua

et al. 2021

ACS Sustainable Chem. Eng.

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The Malaysia petrochemical industry has continued to grow due to its dominance in the global chemical market. The consequential adverse impact on environmental aspects and resource depletion have urged the petrochemical industries to commit to sustainability-driven initiatives such as substitution with renewables and natural processes using biobased feedstock. Nevertheless, many petrochemical industries shy way from investing in such a complex and capital-intensive production process. It is anticipated that capital cost and energy cost can be reduced significantly if the functional group of biobased feedstock matches well with the composition and energy content of the end product. This perspective reviews the technical aspects of the processes for converting vegetable oil-based raw materials into the respective biobased smart drop-in and dedicated chemicals, 1,3-propanediol (1,3-PDO) and lubricant, attributed to the notable market position of Malaysia petrochemical industries for these products. Vegetable oil-based glycerol is a potential bioderived feedstock for the synthesis of 1,3-PDO. Hitherto, the biotechnological conversion process has been commercialized even though its complexity and capital cost can be further reduced by using microbial consortia, which are highly adaptable to the less stringent continuous glycerol fermentation. The comparatively simple chemical conversion process is yet to be commercialized because of impractical catalyst selectivity and stability under severe operating conditions. On another note, vegetable oils have been proven as a promising feedstock for the production of biolubricants. Despite numerous research efforts, setbacks like process complexity and unrecyclable catalysts are still restricting an economically viable large-scale production of biolubricants from vegetable oil. Palm oil which is abundantly available in Malaysia could continue to be the feasible biobased feedstock for the production of 1,3-PDO and biolubricant, provided sustainable agronomic and manufacturing practices were developed for the cultivation of oil palm and production of palm oil, respectively. With an abundant supply of biobased feedstock, the petrochemical industries, with their market position for chemical products, and the palm oil and oleochemical industries should work hand in hand to green Malaysia's economy through the acceleration of the development of sustainable business in biobased chemicals. The mutually beneficial partnership could only be promoted by the realization of relevant government policies.

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Section: Smart Drop-in Biobased 13-propanediolmentioning

confidence: 98%

Palm Oil-Based Chemicals for Sustainable Development of Petrochemical Industries in Malaysia: Progress, Prospect, and Challenges

Chin

Shahruddin

Chua

et al. 2021

ACS Sustainable Chem. Eng.

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“…After pretreatment, the fermentation broth (glycerol as substrate) is a low-concentration aqueous solution (80%-90% water content) of mixed composition (1,3-PDO: 30-100 g/L, main by-product 2,3-butanediol [2,3-BDO]: 10-70 g/L) due to the different wild-type strains used (Table S1). [4][5][6][7][8] The product 1,3-PDO and the main byproduct 2,3-BDO are both diols, which contain two hydroxyl groups and can form strong hydrogen bonds with a large amount of water, which increases the difficulty of obtaining the high-quality 1,3-PDO product (Figure S1; Table S2). 9,10 Various strategies for the separation of 1,3-PDO and 2,3-BDO have been reported, such as distillation, 11 solvent extraction, 12 Jianshuang Zhang and Jinglan Wu contributed equally to this study.…”

Section: Introductionmentioning

confidence: 99%

Introduction of hydrogen bond recognition sites in ZIF‐71 for effective separation of bio‐diols in aqueous solutions

Zhang,

Wu,

Gao

et al. 2023

AIChE Journal

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The bio‐based diols 1,3‐propanediol (1,3‐PDO) and 2,3‐butanediol (2,3‐BDO) can form strong hydrogen bonds with water, which increases the separation difficulty of diols in aqueous solutions. Herein, a series of mixed‐linker ZIF‐71‐clnImx samples with hydrogen bond–van der Waals (vdW) dual recognition sites have been constructed using 4‐chloro‐5‐nitroimidazole (clnIm) linkers to substitute partially for the original 4,5‐dichloroimidazole (dclIm) linkers of the ZIF‐71 framework. The experimental and calculated results show that ZIF‐71‐clnImx with appropriate clnIm substitution proportion (x = 7.6 and 12.8 mol%) can strengthen the interaction between ZIF‐71‐clnImx and 2,3‐BDO and enhance the adsorption and mass transfer of 2,3‐BDO, thus achieving the efficient separation of 2,3‐BDO/1,3‐PDO. Molecular simulation results further verify that 2,3‐BDO can simultaneously form strong H bonds with the –NO2 of the clnIm linker and weak vdW interactions with the –Cl of the dclIm linker, which are the mixed recognition sites for 2,3‐BDO adsorption intensification in aqueous solution for ZIF‐71‐clnImx.

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“…The rise of the biodiesel industry leads to the overproduction of glycerol as a by-product, which now threatens the economic feasibility of the industry (Pan et al, 2019;Kim et al, 2020). This situation has prompted scientists to explore the utilization of glycerol as a carbon source to produce 1,3-propanediol (1,3-PDO), which is a precursor of some important commercial polymers such as polyester and polyurethane (Zhou et al, 2019b;Bao et al, 2020;Chen et al, 2020). 1,3-PDO can be produced by chemical synthesis or biosynthesis using Klebsiella pneumoniae.…”

Section: Introductionmentioning

confidence: 99%

Rational Proteomic Analysis of a New Domesticated Klebsiella pneumoniae x546 Producing 1,3-Propanediol

et al. 2021

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In order to improve the capability of Klebsiella pneumoniae to produce an important chemical raw material, 1,3-propanediol (1,3-PDO), a new type of K. pneumoniae x546 was obtained by glycerol acclimation and subsequently was used to produce 1,3-PDO. Under the control of pH value using Na+ pH neutralizer, the 1,3-PDO yield of K. pneumoniae x546 in a 7.5-L fermenter was 69.35 g/L, which was 1.5-fold higher than the original strain (45.91 g/L). After the addition of betaine, the yield of 1,3-PDO reached up to 74.44 g/L at 24 h, which was 40% shorter than the original fermentation time of 40 h. To study the potential mechanism of the production improvement of 1,3-PDO, the Tandem Mass Tags (TMT) technology was applied to investigate the production of 1,3-PDO in K. pneumoniae. Compared with the control group, 170 up-regulated proteins and 291 down-regulated proteins were identified. Through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis, it was found that some proteins [such as homoserine kinase (ThrB), phosphoribosylglycinamide formyltransferase (PurT), phosphoribosylaminoimidazolesuccinocarboxamide synthase (PurC), etc.] were involved in the fermentation process, whereas some other proteins (such as ProX, ProW, ProV, etc.) played a significant role after the addition of betaine. Moreover, combined with the metabolic network of K. pneumoniae during 1,3-PDO, the proteins in the biosynthesis of 1,3-PDO [such as DhaD, DhaK, lactate dehydrogenase (LDH), BudC, etc.] were analyzed. The process of 1,3-PDO production in K. pneumoniae was explained from the perspective of proteome for the first time, which provided a theoretical basis for genetic engineering modification to improve the yield of 1,3-PDO. Because of the use of Na+ pH neutralizer in the fermentation, the subsequent environmental pollution treatment cost was greatly reduced, showing high potential for industry application in the future.

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Regulation of Pyruvate Formate Lyase-Deficient Klebsiella pneumoniae for Efficient 1,3-Propanediol Bioproduction

Cited by 8 publications

References 27 publications

Palm Oil-Based Chemicals for Sustainable Development of Petrochemical Industries in Malaysia: Progress, Prospect, and Challenges

Palm Oil-Based Chemicals for Sustainable Development of Petrochemical Industries in Malaysia: Progress, Prospect, and Challenges

Introduction of hydrogen bond recognition sites in ZIF‐71 for effective separation of bio‐diols in aqueous solutions

Rational Proteomic Analysis of a New Domesticated Klebsiella pneumoniae x546 Producing 1,3-Propanediol

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