Regulation of protein kinases in steady-state contraction of cat gastric smooth muscle

Sim, Sang-Soo; Baek, Hye-Jung; Yoon, Sung Hyun; Rhie, Duck-Joo; Hahn, Sang-June; Jo, Yang-Hyeok; Kim, Myung-Suk

doi:10.1016/s0014-2999(97)00083-6

Cited by 5 publications

(4 citation statements)

References 31 publications

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“…Many transmitters have been shown to affect contraction of intestinal muscle strips via PKC‐dependent mechanisms. In gastrointestinal muscle, PKC has been shown to be involved in signalling via ACh 6 , SP 7 , angiotensin II 8 , CCK 9 and EGF 9 . Studies on isolated ICC and muscle cells are needed to determine if these effects are in muscle cells or ICC.…”

Section: Discussionmentioning

confidence: 99%

“…Because it was not recognized that segments of circular and longitudinal muscle contain ICC, many studies that have concluded that PKC has a role in muscle may actually include effects in ICC. For example, in gastrointestinal muscle, PKC has been shown to be involved in regulating muscle contraction via acetylcholine (ACh), 6 substance P (SP), 7 angiotensin II, 8 cholecystokinin (CCK) 9 and endothelial growth factor (EGF) 9 . ICC have receptors for ACh, SP, vasoactive intestinal peptide (VIP) and nitric oxide (NO), 10–14 so this signalling could involve ICC.…”

Section: Introductionmentioning

confidence: 99%

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Localization of protein kinase C theta immunoreactivity to interstitial cells of Cajal in guinea‐pig gastrointestinal tract

Southwell

2003

Neurogastroenterology Motil

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In the gastrointestinal tract, interstitial cells of Cajal (ICC) are located between nerve fibres and muscle cells and have a role in neuromuscular transmission and muscle contractility. Protein kinase C (PKC) is involved in modulation of muscle contractility by neurotransmitters, but it is not known if PKC has a role in ICC. There are 11 different PKC isoforms. The presence of PKC isoforms in ICC in guinea-pig gastrointestinal tract was examined using fluorescence immunohistochemistry and confocal microscopy. Segments of guinea-pig stomach, duodenum, ileum, proximal and distal colon were fixed in zambonis fixative. Frozen sections and wholemounts were incubated with anti-PKC antibodies (alpha, beta, delta, epsilon, gamma, iota, lambda, mu, theta) followed by fluorescent secondary antibody. Only PKC theta (theta) immunoreactivity was found in ICC. None of the other PKC isoforms (alpha, beta, delta, epsilon, gamma, iota, lambda, mu) localized to the ICC. PKC theta immunoreactivity was prominent in ICC located between the circular and longitudinal muscle layers (ICC-MY) in all regions except stomach and within the circular muscle (ICC-IM) in the large intestine. PKC theta was not present in ICC in the deep muscular plexus in either duodenum or ileum. PKC theta immunoreactivity was present in the cell body and proximal processes of the ICC. The cells containing PKC theta also contained cKit confirming the cells were ICC. ICC-MY in the ileum also contained the neurokinin (NK) 1 receptor. In conclusion, PKC theta is present in pacemaker ICC, but its function is not yet known. Functional studies will be needed to determine the role of this kinase in ICC. Knowing the second messenger cascades and being able to manipulate subpopulations of ICC will add to our understanding of the molecular and cell biology of ICC networks within the gastrointestinal tract and may ultimately help in understanding the aetiology of some gastrointestinal motor pathologies.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Localization of protein kinase C theta immunoreactivity to interstitial cells of Cajal in guinea‐pig gastrointestinal tract

Southwell

2003

Neurogastroenterology Motil

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“…The Krebs bicarbonate solution was aerated with 95% O 2 -5% CO 2 until the pH was 7.4. Circular muscle strips (1.0 £ 0.2 cm) were prepared from the corpus, cutting at right-angles to the greater curvature (11).…”

Section: Preparation Of Gastric Smooth Muscle Stripsmentioning

confidence: 99%

Effect of Ethanol on Spontaneous Phasic Contractions of Cat Gastric Smooth Muscle

Sim

Choi

Min³

et al. 2002

Scandinavian Journal of Gastroenterology

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“…The Krebs bicarbonate solution was aerated with 95% O 2 -5% CO 2 until the pH was 7.4. Circular muscle strips (1.0 0.2 cm) were prepared from the fundus, cutting at right angles to the greater curvature (22).…”

Section: Preparation Of Gastric Smooth Muscle Stripsmentioning

confidence: 99%

Involvement of Cyclic GMP in Nitric-Oxide-induced Gastric Relaxation: Comparison of the Actions of Cyclic GMP and Cyclic AMP

Sim

Kim

Shim

et al. 2001

Scandinavian Journal of Gastroenterology

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Regulation of protein kinases in steady-state contraction of cat gastric smooth muscle

Cited by 5 publications

References 31 publications

Localization of protein kinase C theta immunoreactivity to interstitial cells of Cajal in guinea‐pig gastrointestinal tract

Localization of protein kinase C theta immunoreactivity to interstitial cells of Cajal in guinea‐pig gastrointestinal tract

Effect of Ethanol on Spontaneous Phasic Contractions of Cat Gastric Smooth Muscle

Involvement of Cyclic GMP in Nitric-Oxide-induced Gastric Relaxation: Comparison of the Actions of Cyclic GMP and Cyclic AMP

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