Involvement of Cyclic GMP in Nitric-Oxide-induced Gastric Relaxation: Comparison of the Actions of Cyclic GMP and Cyclic AMP

Sim, Sang Soo; Kim, Y.-C.; Shim, Hyun Soo; Choi, Ji‐Yeob; Min, Do Sik; Rhie, Duck-Joo; Yoon, Shin Hee; Hahn, Sang June; Kim, M.-S.; Jo, Yang-Hyeok

doi:10.1080/00365520150218011

Cited by 1 publication

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References 27 publications

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“…cAMP and cGMP are jointly responsible for muscle relaxation but the cross‐talk between the two influences the overall muscle response 40,41 . The activation of the cAMP and cGMP‐pathways is the physiological norm in smooth muscle of the gut on which, however, VIP induces a greater generation of cAMP 25,42 .…”

Section: Discussionmentioning

confidence: 99%

Vasoactive intestinal peptide receptor subtypes and signalling pathways involved in relaxation of human stomach

Severi¹,

Tattoli²,

Corleto³

et al. 2006

Neurogastroenterology Motil

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Vasoactive intestinal peptide (VIP) relaxes smooth muscle by interacting with receptors coupled to cAMP- or cGMP-signalling pathways. Their relative contribution to human gastric relaxation is unknown. This study aimed at investigating, in terms of biological activity, receptor expression and related signalling pathways, the action of VIP separately on the human fundus and the antrum. VIP caused greater relaxation of smooth muscle cells (SMC) and strips of the antrum presenting on the former a higher efficacy and potency (ED(50): 0.53 +/- 0.17 nmol L(-1)) than on the fundus (ED(50): 3.4 +/- 1.4 nmol L(-1)). On both fundus and antrum strips, its effect was tetrodotoxin insentitive. Reverse transcriptase-polymerase chain reaction analysis showed the sole expression of VPAC2 and natriuretic peptide clearance receptors, with VPAC2 being more abundant in the antrum. Functional regional differences in receptor-related signalling pathways were found. Activation of the cAMP-pathway by forskolin or its inhibition by adenylate cyclase (2'5'-dideoxyadenosine) or kinase (Rp-cAMPs) inhibitors had more pronounced effects on antrum SMC. Activation of the cGMP-pathway by sodium nitroprusside or its inhibition by guanylate cyclase (LY83583) or kinase (KT5823) inhibitors had more effects on fundus SMC, on which a higher expression of endothelial nitric oxide synthase was found. In conclusion, regional differences in VIP action on human stomach are related to distinct myogenic properties of SMC of the antrum and the fundus.

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Section: Discussionmentioning

confidence: 99%

Vasoactive intestinal peptide receptor subtypes and signalling pathways involved in relaxation of human stomach

Severi¹,

Tattoli²,

Corleto³

et al. 2006

Neurogastroenterology Motil

View full text Add to dashboard Cite

show abstract

Involvement of Cyclic GMP in Nitric-Oxide-induced Gastric Relaxation: Comparison of the Actions of Cyclic GMP and Cyclic AMP

Abstract: cAMP inhibits the contraction mechanism associated with intracellular Ca2+ mobilization as well as extracellular Ca2+ influx, while cGMP inhibits contraction by inhibiting the mechanism associated with extracellular Ca2+ influx.

Cited by 1 publication

References 27 publications

Vasoactive intestinal peptide receptor subtypes and signalling pathways involved in relaxation of human stomach

Vasoactive intestinal peptide receptor subtypes and signalling pathways involved in relaxation of human stomach

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