Regulation of pri-MIRNA processing: mechanistic insights into the miRNA homeostasis in plant

Jodder, Jayanti

doi:10.1007/s00299-020-02660-7

Cited by 17 publications

(11 citation statements)

References 134 publications

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“…Given that miRNAs exert their function by reducing their downstream expression of target genes [24]. The variety of functions of miR-223 has been linked to the suppression of many different target genes in inflammatory pathologies [25,26].…”

Section: Targeting Relationship Between Mir-223-3p and Mek/erkmentioning

confidence: 99%

miR-223-3p inhibits the progression of atherosclerosis via down-regulating the activation of MEK1/ERK1/2 in macrophages

You

Qiao

Ono

et al. 2022

Aging

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Background: microRNAs (miRNAs) have drawn more attention to the progression of atherosclerosis (AS), due to their noticeable inflammation function in cardiovascular disease. Macrophages play a crucial role in disrupting atherosclerotic plaque, thereby we explored the involvement of miR-223-3p in the inflammatory response in macrophages. Methods: RT-qPCR was used to analyze the miR-223-3p levels in carotid arteries and serum of AS patients. ROC curve was used to assess the diagnostic value of miR-223-3p. Movat staining was applied to evaluate the morphological differences. FISH was used to identify the expression of miR-223-3p in macrophages of atherosclerotic lesions. Bioinformatic analysis was performed. Double-immunofluorescence and western blot were performed to assess the inflammatory cytokine secretion and p-ERK1/2. C16-PAF was injected into the culture medium of the miR-223-3p mimic/NC-transfected macrophages with ox-LDL. Results: MiR-223-3p was up-regulated in AS patients and was associated with a higher overall survival rate. MiR-223-3p was co-localized with CD68+ macrophages in vulnerable atherosclerotic lesions. MiR-223-3p mimics decreased atherosclerotic lesions, macrophages numbers whereas increased SMCs numbers in the lesions. The TNF-a immune-positive areas were reduced by miR-223-3p mimics. MAP2K1 was negatively associated with miR-223-3p. MiR-223-3p mimics reduced the inflammation and the MEK1/ERK1/2 signaling pathway in vivo and in vitro. C16-PAF reversed the effects of miR-223-3p mimics on inflammation and ERK1/2 signaling pathway. Conclusions: MiR-223-3p negatively regulates inflammatory responses by the MEK1/ERK1/2 signaling pathway. Our study provides new insight into how miR-223-3p protects against atherosclerosis, representing a broader therapeutic prospect for treating atherosclerosis by miR-223-3p.

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Section: Targeting Relationship Between Mir-223-3p and Mek/erkmentioning

confidence: 99%

miR-223-3p inhibits the progression of atherosclerosis via down-regulating the activation of MEK1/ERK1/2 in macrophages

You

Qiao

Ono

et al. 2022

Aging

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“…The miRISC uncapped and sensitive 3′ cleavage products are degraded by the exonucleases (EXONUCLEASE4 being identified while the other nucleases were still unknown). On the other hand, capped 5′ cleavage products first undergo uridylation by HESO1 [ 32 ] and are then cleaved by RISC-INTERACTING CLEARING 3′-5′ EXORIBONULEASE 1 (RICE1) and (RICE2) [ 33 ] assisted by some RNA exosome cofactors including SKI2, SKI3, and SKI8 [ 34 ] Repression at the translational level: In addition to target messenger RNA cleavage, miRNAs negatively regulate the gene expression through translational repression also [ 35 ]. In the case of plants, although miRNAs and their target mRNAs have mostly near-perfect complementarity in their sequences, nevertheless, the negative regulation via translational repression is extensive [ 36 ].…”

Section: Micrornas and Their Mode Of Actionmentioning

confidence: 99%

Section: Micrornas and Their Mode Of Actionmentioning

confidence: 99%

“…Numerous studies have demonstrated the changes in the expression level of different miRNA families in N limitation conditions in various plant species such as Arabidopsis [ 51 , 52 ], maize [ 53 , 54 ], sorghum [ 55 ], rice [ 32 ], and soybean [ 33 ]. The altered expression pattern of these miRNAs has been shown to play crucial roles in plant adaptive responses to N limitation via the mediation of the expression of their target genes involved in N uptake and remobilization [ 56 ].…”

Section: Biological Functions Of Micrornas In N Metabolismmentioning

confidence: 99%

“…under N deficient conditions in peanut. Analysis of miRNA expression in root tissues of several rice genotypes showed that miR156 and miR447 were overexpressed while miR398 was downregulated in response to N stress [ 32 ]. The different miRNAs which get differentially expressed under N starvation conditions are given in Table 1 .…”

Section: Biological Functions Of Micrornas In N Metabolismmentioning

confidence: 99%

See 2 more Smart Citations

miRNAomic Approach to Plant Nitrogen Starvation

Yousuf¹,

Shabir²,

Hakeem

2021

International Journal of Genomics

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Nitrogen (N) is one of the indispensable nutrients required by plants for their growth, development, and survival. Being a limited nutrient, it is mostly supplied exogenously to the plants, to maintain quality and productivity. The increased use of N fertilizers is associated with high-cost inputs and negative environmental consequences, which necessitates the development of nitrogen-use-efficient plants for sustainable agriculture. Understanding the regulatory mechanisms underlying N metabolism in plants under low N is one of the prerequisites for the development of nitrogen-use-efficient plants. One of the important and recently discovered groups of regulatory molecules acting at the posttranscriptional and translational levels are microRNAs (miRNAs). miRNAs are known to play critical roles in the regulation of gene expression in plants under different stress conditions including N stress. Several classes of miRNAs associated with N metabolism have been identified so far. These nitrogen-responsive miRNAs may provide a platform for a better understanding of the regulation of N metabolism and pave a way for the development of genotypes for better N utilization. The current review presents a brief outline of miRNAs and their regulatory role in N metabolism.

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Regulatory mechanism of the miR172e–LbrAP2 module during the vegetative growth phase transition in Lilium

Feng,

Wang,

et al. 2023

Planta

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Regulation of pri-MIRNA processing: mechanistic insights into the miRNA homeostasis in plant

Cited by 17 publications

References 134 publications

miR-223-3p inhibits the progression of atherosclerosis via down-regulating the activation of MEK1/ERK1/2 in macrophages

miR-223-3p inhibits the progression of atherosclerosis via down-regulating the activation of MEK1/ERK1/2 in macrophages

miRNAomic Approach to Plant Nitrogen Starvation

Regulatory mechanism of the miR172e–LbrAP2 module during the vegetative growth phase transition in Lilium

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