Regulation of photosynthesis by sugars in sugarcane leaves

McCormick, Alistair J.; Cramer, Michael D.; Watt, D. A.

doi:10.1016/j.jplph.2008.01.008

Cited by 80 publications

(46 citation statements)

References 59 publications

(67 reference statements)

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“…Cold-girdle induced sugar accumulation was associated with a decline in photosynthetic activity measures including A, ETR, J max and CE. These changes in photosynthesis were similar to those observed in excised sugarcane leaves supplied with sugars [49]. The application of a cold-girdle also had similar consequences for photosynthesis as the accumulation of sugar through the course of a day.…”

Section: Discussionsupporting

confidence: 74%

Differential Expression of Genes in the Leaves of Sugarcane in Response to Sugar Accumulation

McCormick

Cramer

Watt

2008

Tropical Plant Biol.

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In C 4 sugarcane (Saccharum spp. hybrids), photosynthetic activity has been shown to be regulated by the demand for carbon from sink tissues. There is evidence, from other plant species, that sink-limitation of photosynthesis is facilitated by sugar-signaling mechanisms in the leaf that affect photosynthesis through regulation of gene expression. In this work, we manipulated leaf sugar levels by cold-girdling leaves (5°C) for 80 h to examine the mechanisms whereby leaf sugar accumulation affects photosynthetic activity and assess whether signaling mechanisms reported for other species operate in sugarcane. During this time, sucrose and hexose concentrations above the girdle increased by 77% and 81%, respectively. Conversely, leaf photosynthetic activity (A) and electron transport rates (ETR) decreased by 66% and 54%, respectively. Quantitative expression profiling by means of an Affymetrix GeneChip Sugarcane Genome Array was used to identify genes responsive to cold-girdling (56 h). A number of genes (74) involved in primary and secondary metabolic pathways were identified as being differentially expressed. Decreased expression of genes related to photosynthesis and increased expression of genes involved in assimilate partitioning, cell wall synthesis, phosphate metabolism and stress were observed. Furthermore four probe sets homologous to trehalose 6-phosphate phosphatase (TPP; EC 5.3.1.1) and trehalose 6-phosphate synthase (TPS; EC 2.4.1.15) were up-and down-regulated, respectively, indicating a possible role for trehalose 6-phosphate (T6P) as a putative sugar-sensor in sugarcane leaves.

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Section: Discussionsupporting

confidence: 74%

Differential Expression of Genes in the Leaves of Sugarcane in Response to Sugar Accumulation

McCormick

Cramer

Watt

2008

Tropical Plant Biol.

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“…The fact that photosynthesis was reduced already after the morning peak points to a fast negative feedback regulation. Evidence for a rapid regulatory effect on photosynthesis has been obtained for culms of sugarcane, where Suc feeding caused significantly lower assimilation and electron transport rates already within 2 h (McCormick et al, 2008). This inhibition can indeed occur regularly under normal growth conditions because of source overcapacity, as demonstrated for greenhouse-grown tomato (Solanum lycopersicum) plants, where midday depression of photosynthesis was stronger in spring than in winter because of the higher light intensities (Ayari et al, 2000).…”

Section: Discussion Dynamic Simulation Of Central Carbohydrate Metabomentioning

confidence: 96%

Mathematical Modeling of the Central Carbohydrate Metabolism in Arabidopsis Reveals a Substantial Regulatory Influence of Vacuolar Invertase on Whole Plant Carbon Metabolism

et al. 2010

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A mathematical model representing metabolite interconversions in the central carbohydrate metabolism of Arabidopsis (Arabidopsis thaliana) was developed to simulate the diurnal dynamics of primary carbon metabolism in a photosynthetically active plant leaf. The model groups enzymatic steps of central carbohydrate metabolism into blocks of interconverting reactions that link easily measurable quantities like CO 2 exchange and quasi-steady-state levels of soluble sugars and starch. When metabolite levels that fluctuate over diurnal cycles are used as a basic condition for simulation, turnover rates for the interconverting reactions can be calculated that approximate measured metabolite dynamics and yield kinetic parameters of interconverting reactions. We used experimental data for Arabidopsis wild-type plants, accession Columbia, and a mutant defective in vacuolar invertase, AtbFruct4, as input data. Reducing invertase activity to mutant levels in the wild-type model led to a correct prediction of increased sucrose levels. However, additional changes were needed to correctly simulate levels of hexoses and sugar phosphates, indicating that invertase knockout causes subsequent changes in other enzymatic parameters. Reduction of invertase activity caused a decline in photosynthesis and export of reduced carbon to associated metabolic pathways and sink organs (e.g. roots), which is in agreement with the reported contribution of vacuolar invertase to sink strength. According to model parameters, there is a role for invertase in leaves, where futile cycling of sucrose appears to have a buffering effect on the pools of sucrose, hexoses, and sugar phosphates. Our data demonstrate that modeling complex metabolic pathways is a useful tool to study the significance of single enzyme activities in complex, nonintuitive networks.

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“…Water stress induced decline in photosynthetic rate is mainly caused by a decrease in phosphoenolpyruvate carboxylase (PEPcase) and ribulose-1,5-biphosphate carboxylase (Rubisco) activity (Du et al, 1996; Inman-Bamber and Smith, 2005; Lakshmanan and Robinson, 2014). It is worth to note that photosynthesis rate is also impacted by sugar accumulation in the leaves (McCormick et al, 2008). Under non-stressed condition low leaf sugar content is conducive to photosynthesis, while high sugar content moderates carbon fixation (Goldschmidt and Huber, 1992).…”

Section: Morphological and Physiological Responses Of Sugarcane To Wamentioning

confidence: 99%

Sugarcane Water Stress Tolerance Mechanisms and Its Implications on Developing Biotechnology Solutions

et al. 2017

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Sugarcane is a unique crop with the ability to accumulate high levels of sugar and is a commercially viable source of biomass for bioelectricity and second-generation bioethanol. Water deficit is the single largest abiotic stress affecting sugarcane productivity and the development of water use efficient and drought tolerant cultivars is an imperative for all major sugarcane producing countries. This review summarizes the physiological and molecular studies on water deficit stress in sugarcane, with the aim to help formulate more effective research strategies for advancing our knowledge on genes and mechanisms underpinning plant response to water stress. We also overview transgenic studies in sugarcane, with an emphasis on the potential strategies to develop superior sugarcane varieties that improve crop productivity in drought-prone environments.

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Regulation of photosynthesis by sugars in sugarcane leaves

Cited by 80 publications

References 59 publications

Differential Expression of Genes in the Leaves of Sugarcane in Response to Sugar Accumulation

Differential Expression of Genes in the Leaves of Sugarcane in Response to Sugar Accumulation

Mathematical Modeling of the Central Carbohydrate Metabolism in Arabidopsis Reveals a Substantial Regulatory Influence of Vacuolar Invertase on Whole Plant Carbon Metabolism

Sugarcane Water Stress Tolerance Mechanisms and Its Implications on Developing Biotechnology Solutions

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