Regulation of Nitrate Reductase in Acetabularia mediterranea

Balandin, Teresa; Aparicio, Pedro J.

doi:10.1093/jxb/43.5.625

Cited by 9 publications

(8 citation statements)

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“…This may be explained by the presence of an important constitutive enzyme level found in plants grown solely on NH4 sources. In recent experiments with N-source variation, Balandin and Aparicio (1992) found some appreciable NRA in the green alga Acetabularia mediterranea fed only with NH4 as a sole N-source. These authors postulated that this particularity could represent an advantage for an organism growing in low amounts of nitrate.…”

Section: Enzyme Activity and Distributionmentioning

confidence: 99%

Nitrate reductase and glutamine synthetase activities in relation to growth and nitrogen assimilation in red oak and red ash seedlings: effects of N-forms, N concentration and light intensity

Truax

Gagnon

Chevrier

1994

Trees

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Abstract. The effects of growing seedlings of red oak (Quercus rubra) and red ash (Fraxinus pennsylvanica) with Hoagland solutions containing five N-regimes, differing in the N-forms (NH4, NO3) and concentrations (High and Low), in relation to light intensity were investigated by the utilization of enzymatic markers of the N assimilation pathway, nitrate reductase (NR) and glutamine synthetase (GS). Red oak and red ash showed different patterns of N-assimilation. Red oak seedlings assimilated NO3 in low amounts in their roots and leaves, whereas red ash seedlings assimilated high amounts of NO3, mostly in the leaves. A significant amount of constitutive NR activity was found in red oak seedlings supplied with NH4 N-regime. This could be characteristic of a species adapted to soils that are poor in nitrogen. Root GS activity was lower in red oak seedlings than in red ash seedlings, indicating that the rate of NH4 assimilation differed in these two hardwood species. Low irradiance reduced growth of both hardwood species, but greatly affected the specific leaf area of red ash and reduced NO3 assimilation (when data are expressed per leaf area). Both species reacted similarly to N-regimes in terms of relative growth rate.

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Section: Enzyme Activity and Distributionmentioning

confidence: 99%

Nitrate reductase and glutamine synthetase activities in relation to growth and nitrogen assimilation in red oak and red ash seedlings: effects of N-forms, N concentration and light intensity

Truax

Gagnon

Chevrier

1994

Trees

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“…Inactivation of NR activity and degradation of NR-protein have been observed in microalgae, macroalgae and higher plants undergoing nitrate deficiency or other forms of reduced nitrogen (e.g. ammonium and urea) (Weidner & Kiefer, 1981;Vergara et al, 1998;Solomonson & Barber, 1990;Balandin & Aparicio, 1992;Crawford &Arst, 1993;Vergara et al, 1998;Campbell, 1999;Gao et al, 2000;Chow & Oliveira, 2007;Nicodemus et al, 2008). The NR-inhibiting action mechanism is unknown, although it is thought to occur via feedback-regulation of ammonium assimilation by metabolites, e.g.…”

Section: Nitrate Reductase In Macroalgaementioning

confidence: 99%

Nitrate Assimilation: The Role of In Vitro Nitrate Reductase Assay as Nutritional Predictor

Chow¹

2012

Applied Photosynthesis

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“…Another difference between NR activity in Fucus and other algal species examined to date is the response to ammonium enrichment. Ammonium has been observed to inhibit NR activity, and NR protein synthesis, in several algae (Balandin and Aparicio 1992, Berges et al. 1995, Vergara et al.…”

Section: Novel Patterns Of Nr Activity In Fucus Speciesmentioning

confidence: 99%

“…Hamel (Weidner and Kiefer 1981). Ammonium also suppressed NR protein synthesis in a green macroalga (Balandin and Aparicio 1992) and in a diatom (Vergara et al. 1998).…”

mentioning

confidence: 99%

Distinct patterns of nitrate reductase activity in brown algae: light and ammonium sensitivity in Laminaria digitata is absent in Fucus species¹

Young

Dring

Berges

2007

Journal of Phycology

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Fucus and Laminaria species, dominant seaweeds in the intertidal and subtidal zones of the temperate North Atlantic, experience tidal cycles that are not synchronized with light:dark (L:D) cycles. To investigate how nutrient assimilation is affected by light cycles, the activity of nitrate reductase (NR) was examined in thalli incubated in outdoor tanks with flowing seawater and natural L:D cycles. NR activity in Laminaria digitata (Huds.) Lamour. showed strong diel patterns with low activities in darkness and peak activities near midday. This diel pattern was controlled by light but not by a circadian rhythm. In contrast, there was no diel variation in NR activity in Fucus serratus L., F. vesiculosus (L.) Lamour., and F. spiralis L. either collected directly from the shore or maintained in the outdoor tanks. In laboratory cultures, transfer to continuous darkness suppressed NR activity in L. digitata, but not in F. vesiculosus; continuous light increased NR activity in L. digitata but decreased activity in F. vesiculosus. Furthermore, 4 d enrichment with ammonium (50 lmol AE L )1 pulses), resulted in NR activity declining by >80% in L. digitata, but no significant changes in F. serratus. Seasonal differences in maximum NR activity were present in both genera with activities highest in late winter and lowest in summer. This is the first report of NR activity in any alga that is not strongly regulated by light and ammonium. Because light and tidal emersion do not always coincide, Fucus species may have lost the regulation of NR by light that has been observed in other algae and higher plants.

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Regulation of Nitrate Reductase in Acetabularia mediterranea

Cited by 9 publications

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Nitrate reductase and glutamine synthetase activities in relation to growth and nitrogen assimilation in red oak and red ash seedlings: effects of N-forms, N concentration and light intensity

Nitrate reductase and glutamine synthetase activities in relation to growth and nitrogen assimilation in red oak and red ash seedlings: effects of N-forms, N concentration and light intensity

Nitrate Assimilation: The Role of In Vitro Nitrate Reductase Assay as Nutritional Predictor

Distinct patterns of nitrate reductase activity in brown algae: light and ammonium sensitivity in Laminaria digitata is absent in Fucus species¹

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Regulation of Nitrate Reductase in Acetabularia mediterranea

Cited by 9 publications

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Nitrate reductase and glutamine synthetase activities in relation to growth and nitrogen assimilation in red oak and red ash seedlings: effects of N-forms, N concentration and light intensity

Nitrate reductase and glutamine synthetase activities in relation to growth and nitrogen assimilation in red oak and red ash seedlings: effects of N-forms, N concentration and light intensity

Nitrate Assimilation: The Role of In Vitro Nitrate Reductase Assay as Nutritional Predictor

Distinct patterns of nitrate reductase activity in brown algae: light and ammonium sensitivity in Laminaria digitata is absent in Fucus species1

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Distinct patterns of nitrate reductase activity in brown algae: light and ammonium sensitivity in Laminaria digitata is absent in Fucus species¹