Regulation of Neuronal Thyroid Hormone Receptor α₁ mRNA by Hydrocortisone, Thyroid Hormone and Retinoic Acid

Abstract

The regulation of thyroid hormone receptor Α₁(TRΑ₁) mRNA by hydrocortisone (HC), thyroid hormone (T₃) and retinoic acid (RA) has been studied in mixed and neuronal primary cultures of cells dissociated from fetal rat cerebra. Steady-state levels of TRΑ₁ mRNA were increased as much as 5-fold at 13 days of development by 0.3 µM HC in both mixed and neuronal-enriched cultures. The regulation of TRΑ₁ mRNA by HC appears to be mainly limited to neurons. This conclusion is based on two observations. First, stimulation by HC occurs in cultures highly enriched in neurons at approximately the same time and extent as that seen in mixed-cell cultures (containing neurons, oligodendroglia and astroglia). Second, the stimulation reaches a peak at 13 days in mixed-cell cultures, an age at which neurons but not glia differentiate.

Long-term vitamin A deficiency induces alteration of adult mouse spermatogenesis and spermatogonial differentiation: direct effect on spermatogonial gene expression and indirect effects via somatic cells

“…Multiple studies describe interactions between retinoid and thyroid hormone pathways, in particular interaction with the RXR receptor (67, 68). Depressed expression of thyroid hormone nuclear receptor (TR) has also been reported in the brains of Vitamin-A-Deficient rats (69).…”

Influence of glucocorticoid hormones on the thyroid gland function

“…Взаимодействие ЙТГ с рецепторами в органах-мишенях: гидрокортизон (инкубация смешанной (содержащей нейроны, олигодендроглию и астроглию) и нейронно-обогащенной культур клеток головного мозга крыс с 0,3 мкМ раствором гормона) -увеличение уровня мРНК TRα1 (TR -thyroid receptor: рецептор тиреоидных гормонов) в 5 раз на 13-е сутки инкубации [7]; дексаметазон (внутрибрюшинно 50, 125 и 250 мкг/100 г веса каждые 12 ч в течение 48 ч) -повышение уровня TRβ1 на 52 % в клетках печени адреналэктомированных крыс при использовании дозы 250 мкг/100 г, увеличение содержания мPНК TRβ1 на 43 и 74 % -при 125 и 250 мкг/100 г, рост скорости транскрипции на 255 % -при 250 мкг/100 г, связанный со стимуляцией транскрипционной активности промотора TRβ1, индуцирование связывания белков с последовательностью ДНК сайта промотора TRβ1 [8].…”

Mutual Up-Regulation of Thyroid Hormone and Parathyroid Hormone Receptors in Rat Osteoblastic Osteosarcoma 17/2.8 Cells1

“…1,25-dihydroxyvitamin D, tumor necrosis factor-␣, and retinoic acid down-regulate PTHR and receptor mRNA expression (21,22). Insulin and hydrocortisone increase TR␣ transcripts (23,24), whereas interleukin 1␤, interleukin 6, and tumor necrosis factor-␣ down-regulate TR␣-and TR␤ binding capacity in a liver cell line (25).…”