Regulation of Morphogenesis and Improvement in Shoot Multiplication in Vitex Species Using Thidiazuron

Ahmad, Naseem; Faisal, Mohammad; Hussain, Sheikh Altaf; Anis, Mohammad

doi:10.1007/978-981-10-8004-3_18

Cited by 2 publications

(2 citation statements)

References 9 publications

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“…We observed the same findings in results. The optimum concentration and type of PGRs is different for different explants (Ahmad et al, 2018). In our results, most optimal cytokinine concentration for nodal segment is 1.5 mg/l of BAP and it showed robust-axillary shoots proliferation (100%).…”

Section: Discussionmentioning

confidence: 64%

In Vitro Organogenesis and High-Frequency Plant Regeneration in Vitex Negundo (L) Using Thidiazuron

G.,

S.,

2023

PLANT ARCHIVES

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In this work, a simple, efficient and repeatable protocol was developed for in vitro regeneration via-callus-mediated organogenesis of Vitex negundo using nodal explants. Effects of basal medium and plant growth regulators, with nodal explants were studied. We report optimum response of callus induction from nodal segment explants in the MS medium containing 1.0 mg/l TDZ and 1.5 mg/l BAP. When BAP and TDZ individually or in combination with 2, 4-D and NAA were employed for callus regeneration, shoot and embryo like structure (ELSs) were noted in the callus from nodal explants. The best responses of callus per explants were given by TDZ with NAA. ELSs/explants were ascertained on MS medium with 1.0 mg/l TDZ. TDZ 0.1mg/l and BAP 1.5 mg/l along with NAA were found to perform well for shoot regeneration via callus from nodal explants. Our research data will be significant for in vitro mass propagation of V. negundo via somatic embryogenesis. It can consider as vital and promising off shoot protocol for the conservation of medicinally significance and endangered plant. This work is the first report of a consistent, definitive, and unique protocol for V. negundo regeneration, paving the way for the in vitro preservation of such significant genetic resources and also further allied systems based on such callus-based or embryo-based approaches.

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Section: Discussionmentioning

confidence: 64%

In Vitro Organogenesis and High-Frequency Plant Regeneration in Vitex Negundo (L) Using Thidiazuron

G.,

S.,

2023

PLANT ARCHIVES

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“…This finding indicates that TDZ used on its own and not combined with other plant regulators in the culture medium was most effective in the generation of somaclonal variants resistant to Foc race 1. Thidiazuron is a plant regulator of the cytokinin group, responsible for cell division and used in many studies to induce organogenesis and embryogenesis (PELAH et al, 2002;SHEIBANI et al, 2006;AHMAD et al, 2018). Figure 2.…”

Section: Resultsmentioning

confidence: 99%

Selection of somaclonal variants of the cultivar ‘Prata-Anã’ for resistance to Fusarium oxysporum f. sp. cubense race 1

et al. 2020

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The banana tree is one of the most cultivated fruit globally; however, some diseases significantly affect its production, such as Fusarium wilt. The most appropriate measure for controlling this disease in areas with inoculum pressure is the use of resistant cultivars. Therefore, this study aimed to generate banana somaclones of the cultivar ‘Prata-Anã’ resistant to Fusarium wilt by inducing somaclonal variation. ‘Prata-Anã’ stem apexes were established in vitro in MS culture medium and, on a monthly basis, subcultivated in AIA and adenine sulfate supplemented MS medium with added plant regulators: 6-benzylaminopurine (BAP, 4 ml L-1), Thidiazuron (TDZ, 1 ml L-1), and Paclobutrazol (PBZ, 10 ml L-1). The treatments were: T0: no regulator, T1: BAP, T2: TDZ, T3: PBZ, T4: BAP + TDZ, T5: BAP + PBZ, T6: TDZ + PBZ, and T7: BAP + TDZ + PBZ. After the twelfth subculture, the regenerated plants were planted in boxes containing sterile soil infected with Fusarium oxysporum f. sp. cubense, and evaluated after 90 days for resistance to the pathogen. Somaclonal variants T2-1 and T2-2, generated in Treatment 2, with TDZ, were selected as resistant. This result is promising for the launch of a new Fusarium race 1-resistant banana variety.

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Regulation of Morphogenesis and Improvement in Shoot Multiplication in Vitex Species Using Thidiazuron

Cited by 2 publications

References 9 publications

In Vitro Organogenesis and High-Frequency Plant Regeneration in Vitex Negundo (L) Using Thidiazuron

In Vitro Organogenesis and High-Frequency Plant Regeneration in Vitex Negundo (L) Using Thidiazuron

Selection of somaclonal variants of the cultivar ‘Prata-Anã’ for resistance to Fusarium oxysporum f. sp. cubense race 1

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