In Vitro Organogenesis and High-Frequency Plant Regeneration in Vitex Negundo (L) Using Thidiazuron

Abstract

In this work, a simple, efficient and repeatable protocol was developed for in vitro regeneration via-callus-mediated organogenesis of Vitex negundo using nodal explants. Effects of basal medium and plant growth regulators, with nodal explants were studied. We report optimum response of callus induction from nodal segment explants in the MS medium containing 1.0 mg/l TDZ and 1.5 mg/l BAP. When BAP and TDZ individually or in combination with 2, 4-D and NAA were employed for callus regeneration, shoot and embryo like structure (ELSs) were noted in the callus from nodal explants. The best responses of callus per explants were given by TDZ with NAA. ELSs/explants were ascertained on MS medium with 1.0 mg/l TDZ. TDZ 0.1mg/l and BAP 1.5 mg/l along with NAA were found to perform well for shoot regeneration via callus from nodal explants. Our research data will be significant for in vitro mass propagation of V. negundo via somatic embryogenesis. It can consider as vital and promising off shoot protocol for the conservation of medicinally significance and endangered plant. This work is the first report of a consistent, definitive, and unique protocol for V. negundo regeneration, paving the way for the in vitro preservation of such significant genetic resources and also further allied systems based on such callus-based or embryo-based approaches.