Regulation of maltose fermentation in Saccharomyces carlsbergensis

Berge, A. M. A. ten; Zoutewelle, G.; Poll, K. W. van de

doi:10.1007/bf00271242

Cited by 57 publications

(3 citation statements)

References 11 publications

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“…Early observations suggested that the MAL alleles represented structural rather than regulatory genes (15). Recent reports, however, have described the existence of constitutive regulatory mutations within the MAL genes (18,19,26,27). Also, maltose-nonfermenting strains obtained as segregational nonfermenters (26) Coordinate induction of a-galactosidase and transferase by galactose.…”

Section: Discussionmentioning

confidence: 99%

Genetic co-regulation of galactose and melibiose utilization in Saccharomyces

Kew¹,

Douglas²

1976

J Bacteriol

109

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The gal3 mutation of Saccharomyces, which is associated with an impairment in the utilization of galactose, has been shown to be pleiotropic, causing similar impairments in the utilization of melibiose and maltose. Melibiose utilization and a-galactosidase production are directly controlled by the galactose regulatory elements i, c, and GAL4. The fermentation of maltose and the induction of a-glucosidase are regulated independently of the i,c,GAL4 system. The production of a-galactosidase and galactose-1-phosphate uridyl transferase is coordinate in galactokinaseless strains. Galactose serves as a nonmetabolized, gratuitous inducer of a-galactosidase in strains lacking the genes for one or more of the Leloir pathway enzymes.

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Section: Discussionmentioning

confidence: 99%

Genetic co-regulation of galactose and melibiose utilization in Saccharomyces

Kew¹,

Douglas²

1976

J Bacteriol

109

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“…Although the cryptic copies are insufficient in themselves to permit maltose fermentation, they usually fall into one of two complementation groups capable of complementing each other to give a maltose-positive phenotype (4)(5)(6)(7). In addition, the cryptic copies are capable of complementing mutations in the MAL6 locus (6,8).…”

mentioning

confidence: 99%

“…MALp is thought to encode a positive regulatory factor needed for the induction of maltase and maltose permease, the enzymes required for maltose fermentation (6,8), while MALg has been suggested to encode the enzyme maltase (a-D-glucosidase, EC 3.2.1.20) (2,9). Recently, it was reported that the structural gene for maltose permease maps near a functional MAL gene (refs.…”

mentioning

confidence: 99%

MAL6 of Saccharomyces: a complex genetic locus containing three genes required for maltose fermentation.

Needleman¹,

Kaback²,

Dubin³

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

114

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The MAL6 locus is one of five closely related unlinked loci, any one of which is sufficient for fermentation of maltose in Saccharomyces. Previous genetic analysis indicated that this locus is defined by two complementation groups, MALp and MALg. MALp reportedly is a regulatory gene required for inducible synthesis of the two enzymatic functions needed for fermentation: maltose permease and maltase. We have investigated the physical and genetic structure of the MAL6 locus, which has been isolated on a recombinant DNA plasmid. One subclone of the region, pDF-1, was found to encode a single transcribed region and to contain the MALp gene. A second subclone, pl, was shown to contain the MALg function but surprisingly had not one but two maltose-inducible transcripts. Subclones having only one of these transcribed regions lacked MALg activity. The three transcribed regions have been named MAL61 and MAL62, which correspond to MALg, and MAL63, which corresponds to MALp. This clustered arrangement of a regulatory gene adjacent to the sequences it controls has not previously been described in eukaryotes and is reminiscent of bacterial operons except that the messenger RNA molecules are not polycistronic.

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Effect of Yeast Adaptation to Maltose Utilization on Sugar Uptake During the Fermentation of Brewer's Wort

Ernandes

Williams

Russell

et al. 1993

Journal of the Institute of Brewing

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Yeast adaptation to maltose utilization diminishes the repressing effect of glucose on maltose uptake. Furthermore, it affects both the rates and profiles of maltose, glucose and maltotriose uptake during high cell density brewer's wort fermentation. Yeast cells pre-grown in maltose, as sole carbon source, and harvested while the sugar is still present in the growth medium, are better adapted to utilize maltose. The adapted cells are less sensitive to glucose inhibition, and the uptake of glucose is inhibited in the early stages of fermentation. Cells grown for longer periods and harvested following maltose depletion, lose their ability to preferentially utilize maltose. In addition, they become more sensitive to glucose repression, and are able to utilize glucose faster than the cells harvested when maltose is still present in the medium. During the process of adaptation, maltose controls the induction of its own transport systems and appears to affect the biosynthesis of the glucose transport systems.

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Regulation of maltose fermentation in Saccharomyces carlsbergensis

Cited by 57 publications

References 11 publications

Genetic co-regulation of galactose and melibiose utilization in Saccharomyces

Genetic co-regulation of galactose and melibiose utilization in Saccharomyces

MAL6 of Saccharomyces: a complex genetic locus containing three genes required for maltose fermentation.

Effect of Yeast Adaptation to Maltose Utilization on Sugar Uptake During the Fermentation of Brewer's Wort

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