1984
DOI: 10.1073/pnas.81.9.2811
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MAL6 of Saccharomyces: a complex genetic locus containing three genes required for maltose fermentation.

Abstract: The MAL6 locus is one of five closely related unlinked loci, any one of which is sufficient for fermentation of maltose in Saccharomyces. Previous genetic analysis indicated that this locus is defined by two complementation groups, MALp and MALg. MALp reportedly is a regulatory gene required for inducible synthesis of the two enzymatic functions needed for fermentation: maltose permease and maltase. We have investigated the physical and genetic structure of the MAL6 locus, which has been isolated on a recombin… Show more

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Cited by 114 publications
(90 citation statements)
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“…3) contains a fragment internal to the MAL62 gene. This probe detected a 1.9-kb maltose-inducible transcript in MAL6 strains (11,22). Plasmid pD-5 also detected a 1.9-kb maltose-inducible transcript in the MALl strain 600-1B (Fig.…”
Section: Resultsmentioning
confidence: 99%
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“…3) contains a fragment internal to the MAL62 gene. This probe detected a 1.9-kb maltose-inducible transcript in MAL6 strains (11,22). Plasmid pD-5 also detected a 1.9-kb maltose-inducible transcript in the MALl strain 600-1B (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Plasmid DNA and yeast DNA and RNA isolations as well as Southern and Northern analyses were carried out as previously described except that polyadenylated [poly(A)+] RNA was isolated following a single pass through oligo(dT)-cellulose (11,22).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Strain NCYC 1324 also possessed sequences corresponding to the MAL2 locus. The MAL6 locus, which has been the subject of extensive investigation 8,25,35,43,53,63 , was not detected by the MAL31 probe in the strains studied. In addition to the reactive sequences that could be assigned to specific loci, hybridisation to other bands was observed.…”
Section: (Ixigxmsr Sj Kiriw Mrzspzih Mr Qepxswi Erh Wygvswi Yxmpmwexmsrmentioning
confidence: 99%
“…Total RNA from S. cerevisiae was isolated by the method of Needleman et al (31). RNA was separated in 1.0% agarose gels and transferred to nylon membranes as described previously (7).…”
Section: Methodsmentioning
confidence: 99%