1986
DOI: 10.1128/mcb.6.11.3891
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Structural and functional analysis of the MAL1 locus of Saccharomyces cerevisiae.

Abstract: We describe the isolation of a 22.6-kilobase fragment of DNA containing the MALI locus of Saccharomyces cerevisiae. Our results demonstrate that the MALI locus, like the MAL6 locus, is a complex locus containing three genes. These genes were organized similarly to their MAL6 counterparts. We refer to them as MALI], MAL12, and MAL13 and show that they are functionally homologous to the MAL61 (encoding maltose permease), MAL62 (encoding maltase), and MAL63 (encoding the positive regulator) genes of the MAL6 locu… Show more

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Cited by 90 publications
(76 citation statements)
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“…Each MAL locus is a complex of three genes: GENE1 encodes maltose permease, GENE2 encodes maltase and GENE3 encodes the MALactivator (Charron et al, 1989). All three functional genes are required for a strain to ferment maltose (Charron et al, 1986). However, in some cases only one or two of the three active genes are present and the strains are therefore not able to ferment maltose (Naumov et al, 1994b).…”
Section: Resultsmentioning
confidence: 99%
“…Each MAL locus is a complex of three genes: GENE1 encodes maltose permease, GENE2 encodes maltase and GENE3 encodes the MALactivator (Charron et al, 1989). All three functional genes are required for a strain to ferment maltose (Charron et al, 1986). However, in some cases only one or two of the three active genes are present and the strains are therefore not able to ferment maltose (Naumov et al, 1994b).…”
Section: Resultsmentioning
confidence: 99%
“…The expression of the MALx1 and MALx2 genes required for maltose utilisation is often repressed in the presence of glucose 7,15,27,35 . This phenomenon has a negative effect on the early utilisation of maltose since glucose (10-15 g/L) and fructose (1-2 g/L) are initially present in an all-malt wort.…”
Section: -2863(9'8-32mentioning
confidence: 99%
“…Two kinetically different systems were identified: a high-affinity transporter with a Km of 4 mM and a low-affinity transporter with a Km of 70 to 80 mM. The high-affinity maltose transporter is maltose inducible and is encoded by the MALlI (and/or MAL61) (1,5,7,12,17,18). Each locus is a complex locus consisting of three genes essential for maltose fermentation (5,7,17,18).…”
mentioning
confidence: 99%
“…The high-affinity maltose transporter is maltose inducible and is encoded by the MALlI (and/or MAL61) (1,5,7,12,17,18). Each locus is a complex locus consisting of three genes essential for maltose fermentation (5,7,17,18). GENE 1 has been suggested to encode maltose permease based on genetic analysis of mutations in MAL61, the GENE 1 located at the MAL6 locus (4).…”
mentioning
confidence: 99%