Regulation of lipid metabolism in diabetic rats by Arctium lappa L. polysaccharide through the PKC/NF-κB pathway

Li, Xinpeng; Zhao, Zhihui; Kuang, Pengqun; Shi, Xiaowei; Wang, Zhen; Guo, Lan-Ping

doi:10.1016/j.ijbiomac.2019.06.057

Cited by 40 publications

(24 citation statements)

References 31 publications

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“…PCK8 is a member of the PKC family. As a multifunctional kinase, PKC plays an important role in gene expression and regulation [42]. The activation of PKC can lead to the high expression of NF-kB, which in turn causes in ammation, while H 2 inhibits the activation of the NF-kB signaling pathway by inhibiting the activation of PKC8.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory Effects of Hydrogen in LPS-induced RAW264.7 Cells via Inhibiting NF-κB and MAPKs Signaling Pathways

Zhang

Wang

Jiang

et al. 2021

Preprint

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Hydrogen (H2), a new type of medical gas molecule, which has significant preventive effect on numerous diseases and its anti-inflammatory properties has been proven in previous studies. However, the mechanisms of H2 anti-inflammatory activity in signal transduction pathway or protein level regulation are inadequately inexplicit. In the current study, the effect of H2 on LPS-induced inflammation in RAW 264.7 cells were assessed and its molecular mechanisms were clarified. The in vitro model of inflammation was induced by lipopolysaccharide (LPS) in RAW264.7 cells. Cell viability was evaluated by MTT assay. Protein expression of inflammatory mediators were analyzed by ELISA and Western blot. mRNA levels were detected by RT-qPCR. In addition, RNA sequencing (RNA-seq) was conducted to explore the molecular targets of H2 anti-inflammatory. According to the findings, H2 reversed LPS-induced variety in NO levels and TNF-a production as well as IL-6, IL-10 proteins and related mRNA levels in macrophages. RNA-seq newly discovered that H2 acted on inflammatory signaling molecule protein kinase C 8 (PKC8) and heterodimer activator protein-1 (AP-1). The WB analysis was then used to determine the key proteins in the inflammatory signaling pathway involved in PKC8 and AP-1, which found that H2 inhibited the phosphorylation of key proteins in the NF-kB and MAPKs pathways, thereby the expression of mRNA and inflammatory mediators were affected. The findings of this study show that H2 may serve as a promising anti-inflammatory gas in mitigating inflammatory conditions.

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Section: Discussionmentioning

confidence: 99%

Anti-inflammatory Effects of Hydrogen in LPS-induced RAW264.7 Cells via Inhibiting NF-κB and MAPKs Signaling Pathways

Zhang

Wang

Jiang

et al. 2021

Preprint

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“…Studies have shown that BFO has a wide range of pharmacological activities. BFO has antioxidant properties and the ability to scavenge free radicals ( Jiang et al, 2019 ), it can significantly regulate lipid metabolism in diabetic rats ( Li et al, 2019 ) and exert antithrombotic effects via regulating the ERK/NF-κB pathway ( Qiu et al, 2020 ), and it has anti-inflammatory effects in vivo and in vitro ( Zhang et al, 2019 ; Zhang X. et al, 2020 ), as well as anti-cancer effects ( Xu et al, 2019 ). BFO can also lower fasting blood glucose (FBG) levels and improve glucose tolerance ( Gao Y. et al, 2018 ; Annunziata et al, 2019 ; Yuan et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Burdock Fructooligosaccharide Attenuates High Glucose-Induced Apoptosis and Oxidative Stress Injury in Renal Tubular Epithelial Cells

et al. 2021

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Hyperglycemia-induced apoptosis and oxidative stress injury are thought to play important roles in the pathogenesis of diabetic nephropathy (DN). Attenuating high glucose (HG)-induced renal tubular epithelial cell injury has become a potential approach to ameliorate DN. In recent years, burdock fructooligosaccharide (BFO), a water-soluble inulin-type fructooligosaccharide extracted from burdock root, has been shown to have a wide range of pharmacological activities, including antiviral, anti-inflammatory, and hypolipidemic activities. However, the role and mechanism of BFO in rat renal tubular epithelial cells (NRK-52E cells) have rarely been investigated. The present study investigated the protective effect of BFO on HG-induced damage in NRK-52E cells. BFO could protect NRK-52E cells against the reduced cell viability and significantly increased apoptosis rate induced by HG. These anti-oxidative stress effects of BFO were related to the significant inhibition of the production of reactive oxygen species, stabilization of mitochondrial membrane potential, and increased antioxidant (superoxide dismutase and catalase) activities. Furthermore, BFO increased the expression of Nrf2, HO-1, and Bcl-2 and decreased the expression of Bax. In conclusion, these findings suggest that BFO protects NRK-52E cells against HG-induced damage by inhibiting apoptosis and oxidative stress through the Nrf2/HO-1 signaling pathway.

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“…Its root is edible and rich in amino acids, polyphenols, polysaccharides, volatile oil, avonoids and other nutrients and chemical components (Gao et al 2018;Chen et al 2020). At the same time, roots and seeds of Arctium lappa L. are also traditional Chinese medicine, which have antibacterial, anti-tumor, anti-virus, immune regulation and other pharmacological effects (Chan et al 2011;Li et al 2019). Arctium lappa L. is widely planted in China, and 80% of total output is exported every year.…”

Section: Introductionmentioning

confidence: 99%

Diversity of Endophytic Bacteria Associated with Different Cultivars of Medicinal Plant Arctium Lappa L. and Their Potential for Host Plant Growth Promoting

Liu

Zhang

Gong

et al. 2021

Preprint

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Purpose Arctium lappa L. is one of the medicinal and food homologous plants in China, which is rich in nutrients and medicinal ingredients. The use of plant growth promoting (PGP) endophytic bacteria is a useful alternative in agricultural production to reduce the use of chemical fertilizers. The aim of this study was to analysis the diversity of endophytic bacteria in different cultivars of A. lappa L. collected from two different geographical locations in China and evaluate PGP traits of the isolates and their potential PGP ability in greenhouse condition. Methods Endophytic bacterial community was investigated by culture-dependent and culture-independent methods. Isolates were screened and investigated for multiple PGP traits, and representative strains were inoculated host seedlings to evaluate the growth promoting effect. Results A total of 348 endophytic bacteria were obtained and they distributed into four phyla, 30 genera and 73 different species. In addition, high throughput sequencing revealed more abundant bacterial community, including 17 bacterial phyla, and 207 different known genera. A high proportion of PGP traits were detected, including production of indole acetic acid, siderophore, ammonia and phosphate solubilization. Four representative strains with multiple PGP traits of the most prevalent genera were further selected for host inoculation and growth promoting evaluation, and they significantly increase seedlings length, root length and fresh weight. Conclusion This study demonstrated that A. lappa L. harbors abundant endophytic bacteria, and plant genotype and geographical origin affect their composition. Moreover, some endophytic bacteria showed good potential for the development of microbial fertilizer in the future.

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Regulation of lipid metabolism in diabetic rats by Arctium lappa L. polysaccharide through the PKC/NF-κB pathway

Cited by 40 publications

References 31 publications

Anti-inflammatory Effects of Hydrogen in LPS-induced RAW264.7 Cells via Inhibiting NF-κB and MAPKs Signaling Pathways

Anti-inflammatory Effects of Hydrogen in LPS-induced RAW264.7 Cells via Inhibiting NF-κB and MAPKs Signaling Pathways

Burdock Fructooligosaccharide Attenuates High Glucose-Induced Apoptosis and Oxidative Stress Injury in Renal Tubular Epithelial Cells

Diversity of Endophytic Bacteria Associated with Different Cultivars of Medicinal Plant Arctium Lappa L. and Their Potential for Host Plant Growth Promoting

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