Regulation of lipid metabolism and peroxisome proliferator-activated receptors in rainbow trout adipose tissue by lipolytic and antilipolytic endocrine factors

Cruz-García, Lourdes; Sánchez-Gurmaches, Joan; Monroy, Mario Muñoz; Gutiérrez, Joaquím; Navarro, Isabel

doi:10.1016/j.domaniend.2014.11.002

Cited by 7 publications

(3 citation statements)

References 72 publications

(100 reference statements)

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“…PPARγ is critical for the regulation of lipogenesis and lipid storage, and PPARα has been implicated in fatty acid catabolism (Ribet et al, 2010;Minghetti et al, 2011). The findings of the present study indicate that insulin injection significantly influences mRNA levels of PPARα and PPARγ, suggesting that they mediate the insulin-induced change of lipid metabolism, in agreement with other studies (Zhuo et al, 2014;Cruz-Garcia et al, 2015;Zheng et al, 2015b). However, we also found some differences between our in vivo study and the in vitro study (Zhuo et al, 2014) in the insulin-induced change in mRNA levels of these transcription factors.…”

Section: Discussionsupporting

confidence: 91%

Effect and the related signaling pathways of insulin influencing lipid metabolism in yellow catfishPelteobagrus fulvidraco

Zhuo

Luo

Pan

et al. 2015

Journal of Experimental Biology

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The influence of insulin on hepatic metabolism in fish is not well understood. The present study was therefore conducted to investigate the effects of insulin on lipid metabolism, and the related signaling pathways, in the yellow catfish Pelteobagrus fulvidraco. Hepatic lipid and intracellular triglyceride (TG) content, the activity and expression levels of several enzymes and the mRNA expression of transcription factors (PPARα and PPARγ) involved in lipid metabolism were determined. Troglitazone, GW6471, fenofibrate and wortmannin were used to explore the signaling pathways by which insulin influences lipid metabolism. Insulin tended to increase hepatic lipid accumulation, the activity of lipogenic enzymes (6PGD, G6PD, ME, ICDH and FAS) and mRNA levels of FAS, G6PD, 6PGD, CPT IA and PPARγ, but down-regulated PPARα mRNA level. The insulin-induced effect could be stimulated by the specific PPARγ activator troglitazone or reversed by the PI3 kinase/Akt inhibitor wortmannin, demonstrating that signaling pathways of PPARγ and PI3 kinase/Akt were involved in the insulin-induced alteration of lipid metabolism. The specific PPARα pathway activator fenofibrate reduced insulininduced TG accumulation, down-regulated the mRNA levels of FAS, G6PD and 6PGD, and up-regulated mRNA levels of CPT IA, PPARα and PPARγ. The specific PPARα pathway inhibitor GW6471 reduced insulin-induced changes in the expression of all the tested genes, indicating that PPARα mediated the insulin-induced changes of lipid metabolism. The present results contribute new knowledge on the regulatory role of insulin in hepatic metabolism in fish.

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Section: Discussionsupporting

confidence: 91%

Effect and the related signaling pathways of insulin influencing lipid metabolism in yellow catfishPelteobagrus fulvidraco

Zhuo

Luo

Pan

et al. 2015

Journal of Experimental Biology

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“…In contrast to the liver, LPS administration caused a significant increase in the expression of PPARs, mostly PPARβ and PPARγ, in seabream adipose tissue. This result is contrary to the reported down-regulation of PPARβ by LPS administration in rainbow trout adipose tissue [ 47 ] and by the direct effects of recombinant human TNFα (rhTNFα) in seabream adipocytes in vitro [ 48 ]. Whether there are species-specific differences in the regulation of PPARβ and PPARγ by LPS in teleosts needs to be investigated further.…”

Section: Discussioncontrasting

confidence: 88%

In Vivo Effects of Lipopolysaccharide on Peroxisome Proliferator-Activated Receptor Expression in Juvenile Gilthead Seabream (Sparus Aurata)

Antonopoulou

Kaitetzidou

Castellana

et al. 2017

Biology

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Fish are constantly exposed to microorganisms in the aquatic environment, many of which are bacterial pathogens. Bacterial pathogens activate the innate immune response in fish involving the production of pro-inflammatory molecules that, in addition to their immune-related role, can affect non-immune tissues. In the present study, we aimed at investigating how inflammatory responses can affect metabolic homeostasis in the gilthead seabream (Sparus aurata), a teleost of considerable economic importance in Southern European countries. Specifically, we mimicked a bacterial infection by in vivo administration of lipopolysaccharide (LPS, 6 mg/kg body weight) and measured metabolic parameters in the blood and, importantly, the mRNA expression levels of the three isotypes of peroxisome proliferator activated receptors (PPARα, β, and γ) in metabolically-relevant tissues in seabream. PPARs are nuclear receptors that are important for lipid and carbohydrate metabolism in mammals and that act as biological sensors of altered lipid metabolism. We show here that LPS-induced inflammatory responses result in the modulation of triglyceride plasma levels that are accompanied most notably by a decrease in the hepatic mRNA expression levels of PPARα, β, and γ and by the up-regulation of PPARγ expression only in adipose tissue and the anterior intestine. In addition, LPS-induced inflammation results in an increase in the hepatic mRNA expression and protein activity levels of members of the mitogen-activated protein kinase (MAPK) family, known in mammals to regulate the transcription and activity of PPARs. Our results provide evidence for the involvement of PPARs in the metabolic response to inflammatory stimuli in seabream and offer insights into the molecular mechanisms underlying the redirection of metabolic activities under inflammatory conditions in vertebrates.

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“…Insulin acts as a growth promoter and affects lipid metabolism by inducing adipocytes differentiation and increasing adipose fat stores in red sea bream (Pagrus major), Atlantic salmon (Salmo salar), and large yellow croaker (Pseudosciaena crocea R.) (Oku et al, 2006;Sánchez-Gurmaches et al, 2011;Wang et al, 2012). Insulin promoted rainbow trout preadipocyte differentiation and stimulated lpl gene expression in proliferating and freshly isolated adipocytes of the same species (Bouraoui et al, 2012;Cruz-Garcia et al 2015). However, insulin did not seem to increase lipid accumulation during the differentiation phase on rainbow trout (Salmerón et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Gilthead seabream (Sparus aurata) in vitro adipogenesis and its endocrine regulation by leptin, ghrelin, and insulin

Basto-Silva

Balbuena-Pecino

Oliva-Teles

et al. 2020

Comparative Biochemistry and Physiology Part A: Molecular &

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Regulation of lipid metabolism and peroxisome proliferator-activated receptors in rainbow trout adipose tissue by lipolytic and antilipolytic endocrine factors

Cited by 7 publications

References 72 publications

Effect and the related signaling pathways of insulin influencing lipid metabolism in yellow catfishPelteobagrus fulvidraco

Effect and the related signaling pathways of insulin influencing lipid metabolism in yellow catfishPelteobagrus fulvidraco

In Vivo Effects of Lipopolysaccharide on Peroxisome Proliferator-Activated Receptor Expression in Juvenile Gilthead Seabream (Sparus Aurata)

Gilthead seabream (Sparus aurata) in vitro adipogenesis and its endocrine regulation by leptin, ghrelin, and insulin

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