Regulation of laryngeal squamous cell cancer progression by the lncRNA H19/miR-148a-3p/DNMT1 axis

Wu, Tianyi; Qu, Lingmei; He, Gang; Tian, Linli; Li, Liang; Zhou, Han; Qian, Jin; Ren, Jianlin; Wang, Yu; Wang, Jingting; Kan, Xuan; Liu, Ming; Shen, Jia; Guo, Mian; Sun, Yanan

doi:10.18632/oncotarget.7270

Cited by 124 publications

(102 citation statements)

References 37 publications

(33 reference statements)

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“…2A). Previous studies have demonstrated that H19 siRNA inhibited the cell viability of cancer cells [32, 33], and we obtained the consistent results (Fig. 2B).…”

Section: Resultssupporting

confidence: 90%

Huaier Extract Inhibits Breast Cancer Progression Through a LncRNA-H19/MiR-675-5p Pathway

Wang

Chen

et al. 2017

Cell Physiol Biochem

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Background/Aims: Increasing evidence indicates that Huaier extract has promising therapeutic effects against cancer. However, the mechanisms that underlie its anti-tumor effects remain unclear. In recent years, various studies have shown that long noncoding RNAs (lncRNAs) play a critical role in the regulation of cancer development and progression. Here, we explored the role of lncRNAs in Huaier-induced tumor suppression. Methods: Microarray profiling was performed to identify the candidate lncRNAs affected by Huaier extract. Quantitative realtime PCR (qPCR) was used to evaluate the transfection efficiency and the influence of Huaier extract on H19 expression. The effect of Huaier extract on the cell viability was examined by MTT. Moreover, the rates of apoptotic cells were detected using flow-cytometric analysis. Western blot analysis was applied to show the protein levels of CBL. Results: Microarray data derived from Huaier-treated breast cancer cells identified H19 as a potential target. Huaier extract reduced the expression of H19. The over-expression of H19 inhibited the cytotoxic effects of Huaier extract; in contrast, reduced H19 expression enhanced the function of Huaier extract. MiR-675-5p was identified as a mature product of H19. Moreover, Huaier extract reduced the miR-675-5p expression. Upregulating miR-675-5p reversed the inhibitory effects of Huaier extract, whereas downregulating miR-675-5p sensitized breast cancer cells to the effect of Huaier extract. In addition, Huaier extract increased the expression of CBL protein, a direct target of miR-675-5p. Conclusion: Collectively, the data demonstrate that Huaier extract reduces viability and induces apoptosis in breast cancer cells via H19-miR-675-5p-CBL axis regulation.

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“…2A). Previous studies have demonstrated that H19 siRNA inhibited the cell viability of cancer cells [32, 33], and we obtained the consistent results (Fig. 2B).…”

Section: Resultssupporting

confidence: 90%

Huaier Extract Inhibits Breast Cancer Progression Through a LncRNA-H19/MiR-675-5p Pathway

Wang

Chen

et al. 2017

Cell Physiol Biochem

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“…83 In laryngeal squamous cell carcinoma, DNMT1 is a target of miR-148a-3p, which inhibits cellular DNA methylation. 84 In colorectal cancer, miR-124 and miR-506 are downregulated, and both target DNMT3b directly and DNMT1 indirectly. 85 In addition, DNMT3a has been identified as a direct target of miR-101 in astrocytoma cells, and miR-101 suppresses PRDM16 expression by targeting DNMT3a at the PRDM16 core promoter.…”

Section: How Do Mirnas Regulate Dna Methylation?mentioning

confidence: 99%

Mutual regulation of microRNAs and DNA methylation in human cancers

Wang¹,

Wu²,

Claret

2017

Epigenetics

117

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microRNAs (miRNAs) and DNA methylation are the 2 epigenetic modifications that have emerged in recent years as the most critical players in the regulation of gene expression. Compelling evidence has indicated the roles of miRNAs and DNA methylation in modulating cellular transformation and tumorigenesis. miRNAs act as negative regulators of gene expression and are involved in the regulation of both physiologic conditions and during diseases, such as cancer, inflammatory diseases, and psychiatric disorders, among others. Meanwhile, aberrant DNA methylation manifests in both global genome changes and in localized gene promoter changes, which influences the transcription of cancer genes. In this review, we described the mutual regulation of miRNAs and DNA methylation in human cancers. miRNAs regulate DNA methylation by targeting DNA methyltransferases or methylation-related proteins. On the other hand, both hyper-and hypo-methylation of miRNAs occur frequently in human cancers and represent a new level of complexity in gene regulation. Therefore, understanding the mechanisms underlying the mutual regulation of miRNAs and DNA methylation may provide helpful insights in the development of efficient therapeutic approaches.

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“…Overexpression of miR-148a-3p reduced proliferation and induced apoptosis in OS cells [25]; miR-148a-3p is frequently downregulated and miR-148a-3p inhibits cell proliferation and epithelial mesenchymal transition (EMT) by regulating ERBB3/ AKT2/c-myc and ERBB3/AKT2/Snail signaling in bladder cancer [26]; miR-148a-3p reduced cell migration, invasion and proliferation by targeting DNA methyltransferase enzyme (DNMT1) and runt-related transcription factor 3 (RUNX3) in laryngeal squamous cell carcinoma (LSCC) [27,28]; circling miR-148a-3p has been reported to serve as tumor biomarker [29][30][31]. However, the roles of miR148a-3p in OS has not been reported.…”

Section: Discussionmentioning

confidence: 99%

MiR-148a-3p suppresses cell proliferation, migration and invasion by targeting PIK3CA in human osteosarcoma cells

Wang¹,

Zhou²,

Zhao³

et al. 2018

Oncotarget

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Osteosarcoma (OS) is the most common type of primary malignant bone tumor and is characterized by complex genetic changes and resistance to conventional treatments. In this study, the role of miR-148a-3p in the progression and metastasis of OS was investigated. Firstly, it was found that the expression of miR-148a-3p was down-regulated in OS tissues and cell lines compared with that in adjacent non-neoplastic bone tissues and OS cell line. In addition, it was found that miR148a-3p inhibited cell proliferation, migration and invasion in MG-63 and U2OS cells and tumorigenicity in nude mice. Meanwhile, bioinformatic analysis suggested that phosphatidylinositol-4, 5-bisphosphate 3-kinase, catalytic subunit alpha (PIK3CA) gene is a potential target of miR-148a-3p. Then, it was found that PIK3CA can be directly regulated by miR-148a-3p via luciferase assay and western blot assay. Furthermore, PIK3CA knockdown induced effects on OS cell lines similar to those induced by miR-148a-3p. Taken together, these data suggest that miR-148a-3p suppresses cell proliferation, migration and invasion and PIK3CA was involved in miR-148a-3p associated biological functions in OS. Commonly down-regulated miR148a-3p may act as a tumor suppressor and a novel therapeutic strategy by downregulating PIK3CA expression.

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Regulation of laryngeal squamous cell cancer progression by the lncRNA H19/miR-148a-3p/DNMT1 axis

Cited by 124 publications

References 37 publications

Huaier Extract Inhibits Breast Cancer Progression Through a LncRNA-H19/MiR-675-5p Pathway

Huaier Extract Inhibits Breast Cancer Progression Through a LncRNA-H19/MiR-675-5p Pathway

Mutual regulation of microRNAs and DNA methylation in human cancers

MiR-148a-3p suppresses cell proliferation, migration and invasion by targeting PIK3CA in human osteosarcoma cells

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