Regulation of Intestinal Epithelial Calcium Transport Proteins by Stanniocalcin-1 in Caco2 Cells

Abstract

Stanniocalcin-1 (STC1) is a calcium and phosphate regulatory hormone. However, the exact molecular mechanisms underlying how STC1 affects Ca2+ uptake remain unclear. Here, the expression levels of the calcium transport proteins involved in transcellular transport in Caco2 cells were examined following over-expression or inhibition of STC1. These proteins include the transient receptor potential vanilloid members (TRPV) 5 and 6, the plasma membrane calcium ATPase 1b (PMCA1b), the sodium/calcium exchanger (NCX1), and the vitamin D receptor (VDR). Both gene and protein expressions of TRPV5 and TRPV6 were attenuated in response to over-expression of STC1, and the opposite trend was observed in cells treated with siRNASTC1. To further investigate the ability of STC1 to influence TRPV6 expression, cells were treated with 100 ng/mL of recombinant human STC1 (rhSTC1) for 4 h following pre-transfection with siRNASTC1 for 48 h. Intriguingly, the increase in the expression of TRPV6 resulting from siRNASTC1 was reversed by rhSTC1. No significant effect of STC1 on the expression of PMCA1b, NCX1 or VDR was observed in this study. In conclusion, the effect of STC1 on calcium transport in intestinal epithelia is due to, at least in part, its negative regulation of the epithelial channels TRPV5/6 that mediate calcium influx.

Androgen deprivation therapy promotes an obesity-like microenvironment in periprostatic fat

“…The third top ranked gene (included in the obesity signature) is a key regulator of hormonal homeostasis ( DHRS9 ), which is able to convert 3-alpha-tetrahydroprogesterone to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone in the cytoplasm (47); also, it is a marker for regulatory macrophages (48). Regulatory genes for calcium homeostasis were also present, including S100 calcium-binding protein A1 ( S100A1 ) and stanniocalcin 2 ( STC2 ), which regulate renal and intestinal calcium and phosphate transport (49).

Figure 4GSEA enrichment plot showing the significant enrichment of the obesity signature among the most differentially transcribed genes.

…”

Trichostatin A Induces Autophagy in Cervical Cancer Cells by Regulating the PRMT5-STC1-TRPV6-JNK Pathway

“…Yeung et al [39] reported that patients with a high level of STC1 had smaller tumours. It was reported that STC1 is a potential epithelium-derived relaxing factor in asthma [40] and that STC1 decreases the expression of TRPV6 in Caco2 cells [29]. Law et al [41] found that the histone deacetylase inhibitor TSA induces apoptosis through the regulation of STC1 in HT29 cells.…”

“…A recent study found that PRMT5 can bind to the promoter region of STC1 and reduce STC1 expression in breast cancer [27]. In addition, elevated STC1 can activate JNK signalling in breast cancer, and the inhibition of STC1 leads to increased levels of TRV6 in cervical cancer, indicating possible interactions among the STC1, TRPV6, and JNK signalling pathways [28, 29]. However, the regulatory mechanisms of the STC1 and TRPV6/JNK axes involved in cervical cancer are still unknown.…”

Microbiota Alters Urinary Bladder Weight and Gene Expression

“…Calcium serves as a second messenger that propagates numerous intracellular and extracellular signals, regulating cell proliferation and apoptosis as well as enzyme activity [18]. Stc1 gene has a role in calcium and phosphate cell homeostasis [19], its expression was upregulated in germ-free mice. Calcium channel regulator Rrad gene was downregulated in GF mice, while Acng5, a gene coding for a calcium channel protein, was upregulated in GF mouse bladder tissue.…”