Regulation of Intestinal Alkaline Phosphatase by Dietary Phosphate

Abstract

Day-old chicks were fed practical rations containing varying levels of inorganic phosphate (Pi) in an attempt to study the regulation of intestinal alkaline phosphatase by phosphate. Low dietary Pi (0.16%) increased the enzyme activity by 50% compared to adequate Pi (0.48%). The enzyme activities were significantly reversed 8 days after reversing the diets. In another experiment chicks were fed 0.16 or 0.80%) Pi for 4 weeks and killed when full-fed, or after 3 days' starvation. In the full-fed birds low dietary Pi reduced the intestinal nuclear Pi and doubled the intestinal enzyme activity. Nuclear Pi was found to be inversely correlated with alkaline phosphatase in the fed animals. No such effects were found in the starved birds.

Absence d'effet de la vitamine D sur la phytase et la phosphatase alcaline intestinales : relation avec l'absorption du phosphore phytique chez le porc

“…et la phosphatase alcaline (E.C.3.1.3.1.) sont susceptibles d'intervenir dans l'hydrolyse des phytates (McCuaig et Motzok, 1972 ;Davies, Ritcey et Motzok, 1970 ; Flett, 1978) et dans l'absorption du phosphore (Shirazi, Beechey et Butterworth, 1981 ; Ulmann, 1982 ;Kempson et a/., 1979 ;Birge et Avioli, 19811. Chez le rat ou le poulet, l'activité de l'une et l'autre enzymes augmente avec l'apport de vitamine D tant in vivo (Steenbock et a/., 1953 ; Pileggi, De Luca et Steenbock, 1955 ; Davies, Ritcey et Motzok, 1970 ;Roberts et Yudkins, 1961 ;Bhandari, 1980 ;Valinietse et Bauman, 1984) qu'in vitro (Peterlik et Wasserman, 1980 ;Chan et Atkins, 1983 ; Haussler, Nagode et Rasmussen, 1970 ;Bikle et al, 1979 ; Lacour, 1979).…”

Interactions of Ca, P, Zn and Alkaline Phosphatase in the Chick

“…Phosphorus in excess had no effect (Mc-Cuaig et al, 1972). A mechanism for the action of low phosphate diets has been proposed (McCuaig and Motzok, 1972a).…”