Regulation of inflammatory response in human chondrocytes by lentiviral mediated RNA interference against S100A10

Song, Changzhi; Zhou, Xigeng; Dong, Qirong; Fan, Rengen; Wu, Guangzhou; Ji, Biao; Meng, Qingbing; Zheng, Ming‐Hua

doi:10.1007/s00011-012-0519-6

Cited by 16 publications

(12 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As for S100 proteins, PRRSV infection promoted secretion of S100A10. Previous studies have demonstrated that knockdown of S100A10 inhibits the secretion of inflammatory cytokines and S100A10 could interact with annexin A2 (AnxA2), which was implicated in the process of virus entry and replication . Our data show that AnxA2 was also upregulated in response to PRRSV, indicating that S100A10 and AnxA2 may be involved in the inflammatory response and/or replication process of PRRSV.…”

Section: Discussionsupporting

confidence: 58%

SILAC-based quantitative proteomic analysis of secretome of Marc-145 cells infected with porcine reproductive and respiratory syndrome virus

et al. 2016

View full text Add to dashboard Cite

Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of PRRS, which causes severe reproductive failure in sows, respiratory disease in young and growing pigs, and enormous economic losses to the global swine industry. In this study, SILAC combined with MS/MS was used to quantitatively identify the secretory proteins differentially expressed in PRRSV-infected Marc-145 cells compared with mock-infected controls. In total, we identified 204 secretory proteins showing significant differences in infected cells (163 upregulated, 41 downregulated). Intensive bioinformatic analysis of secretome data revealed that PRRSV infection strongly activated nonclassical protein secretion, especially vesicle-mediated release of exosomal proteins, including different danger-associated molecular pattern molecules and the majority of secreted proteins involved in protein binding and transport, regulation of response to stimulus, metabolic processes, and immune responses. According to the functional proteins analysis, we speculate that proteins functioning in binding, transport, and the immune response are exploited by PRRSV to facilitate virus replication and immune evasion. Our study for the first time analyzes the secretory protein profile of PRRSV-infected Marc-145 cells and provides valuable insight into the host response to PRRSV infection.

show abstract

Section: Discussionsupporting

confidence: 58%

SILAC-based quantitative proteomic analysis of secretome of Marc-145 cells infected with porcine reproductive and respiratory syndrome virus

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The upregulated transcripts comprise chemokine (C-X-C motif) ligand 13, CXCL13 (FC 79.22), CXCL9/MIG (FC 11.16), chemokine (C-C motif) ligand 18, CCL18 (FC 20.32), interleukin 1 receptor, type I, IL1R1 [30,31] (FC 5.67), oxidized low density lipoprotein receptor 1, OLR1/LOX1 (FC 83.1), S100 calcium binding protein namely S100B [32] (FC 7.8) and S100A102 [33] (FC 2.21), fibronectin1, FN1 [34] (FC 405.4), tumour necrosis factor alpha TNF (FC 3.5).…”

Section: Resultsmentioning

confidence: 99%

Gene Expression Profiling in Peripheral Blood Cells and Synovial Membranes of Patients with Psoriatic Arthritis

et al. 2015

View full text Add to dashboard Cite

BackgroundPsoriatic arthritis (PsA) is an inflammatory arthritis whose pathogenesis is poorly understood; it is characterized by bone erosions and new bone formation. The diagnosis of PsA is mainly clinical and diagnostic biomarkers are not yet available. The aim of this work was to clarify some aspects of the disease pathogenesis and to identify specific gene signatures in paired peripheral blood cells (PBC) and synovial biopsies of patients with PsA. Moreover, we tried to identify biomarkers that can be used in clinical practice.MethodsPBC and synovial biopsies of 10 patients with PsA were used to study gene expression using Affymetrix arrays. The expression values were validated by Q-PCR, FACS analysis and by the detection of soluble mediators.ResultsSynovial biopsies of patients showed a modulation of approximately 200 genes when compared to the biopsies of healthy donors. Among the differentially expressed genes we observed the upregulation of Th17 related genes and of type I interferon (IFN) inducible genes. FACS analysis confirmed the Th17 polarization. Moreover, the synovial trascriptome shows gene clusters (bone remodeling, angiogenesis and inflammation) involved in the pathogenesis of PsA. Interestingly 90 genes are modulated in both compartments (PBC and synovium) suggesting that signature pathways in PBC mirror those of the inflamed synovium. Finally the osteoactivin gene was upregulared in both PBC and synovial biopsies and this finding was confirmed by the detection of high levels of osteoactivin in PsA sera but not in other inflammatory arthritides.ConclusionsWe describe the first analysis of the trancriptome in paired synovial tissue and PBC of patients with PsA. This study strengthens the hypothesis that PsA is of autoimmune origin since the coactivity of IFN and Th17 pathways is typical of autoimmunity. Finally these findings have allowed the identification of a possible disease biomarker, osteoactivin, easily detectable in PsA serum.

show abstract

“…49 Recent studies reported on special functions of S100 subtypes in chondrocytes. S100A10 seems to play a role in inflammatory responses in human chondrocytes, 50 whereas S100A12 seems to be involved in the pathogenesis of osteoarthritis by upregulating matrix metalloproteinase 13 (MMP13) and vascular endothelial growth factor (VEGF) both associated with the progression of osteoarthritis. 51 These observations are in line with the statement of Donato et al that particular S100 proteins could be induced in pathological circumstances in a cell type that does not express it in normal physiological conditions.…”

Section: Discussionmentioning

confidence: 99%

Three-dimensional scaffold-free fusion culture: the way to enhance chondrogenesis of in vitro propagated human articular chondrocytes

et al. 2013

View full text Add to dashboard Cite

Cartilage regeneration based on isolated and culture-expanded chondrocytes has been studied in various in vitro models, but the quality varies with respect to the morphology and the physiology of the synthesized tissues. The aim of our study was to promote in vitro chondrogenesis of human articular chondrocytes using a novel three-dimensional (3-D) cultivation system in combination with the chondrogenic differentiation factors transforming growth factor beta 2 (TGF-β2) and L-ascorbic acid. Articular chondrocytes isolated from six elderly patients were expanded in monolayer culture. A single-cell suspension of the dedifferentiated chondrocytes was then added to agar-coated dishes without using any scaffold material, in the presence, or absence of TGF-β2 and/or L-ascorbic acid. Three-dimensional cartilage-like constructs, called single spheroids, and microtissues consisting of several spheroids fused together, named as fusions, were formed. Generated tissues were mainly characterized using histological and immunohistochemical techniques. The morphology of the in vitro tissues shared some similarities to native hyaline cartilage in regard to differentiated S100-positive chondrocytes within a cartilaginous matrix, with strong collagen type II expression and increased synthesis of proteoglycans. Finally, our innovative scaffold-free fusion culture technique supported enhanced chondrogenesis of human articular chondrocytes in vitro. These 3-D hyaline cartilage-like microtissues will be useful for in vitro studies of cartilage differentiation and regeneration, enabling optimization of functional tissue engineering and possibly contributing to the development of new approaches to treat traumatic cartilage defects or osteoarthritis.

show abstract

Regulation of inflammatory response in human chondrocytes by lentiviral mediated RNA interference against S100A10

Abstract: Our investigation demonstrated that S100A10 might be considered as a potential target for anti-inflammatory treatment.

Cited by 16 publications

References 42 publications

SILAC-based quantitative proteomic analysis of secretome of Marc-145 cells infected with porcine reproductive and respiratory syndrome virus

SILAC-based quantitative proteomic analysis of secretome of Marc-145 cells infected with porcine reproductive and respiratory syndrome virus

Gene Expression Profiling in Peripheral Blood Cells and Synovial Membranes of Patients with Psoriatic Arthritis

Three-dimensional scaffold-free fusion culture: the way to enhance chondrogenesis of in vitro propagated human articular chondrocytes

Contact Info

Product

Resources

About