Regulation of human natural killing by levamisole

Shau, Hungyi; Dawson, Jeffrey R.

doi:10.1007/bf00200196

Cited by 5 publications

(4 citation statements)

References 21 publications

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“…The correlation of granule content and cytolytic capacity within NK cells has been well documented (21)(22)(23)(24)(25)(26)(27). However, it is unclear how much degranulation is sufficient for lysis and, for that matter, to what extent other nongranular mechanisms contribute.…”

Section: Determination Of Nk Binder-and Killer-cell Frequencies Bymentioning

confidence: 99%

Purification and characterization of cytolytic and noncytolytic human natural killer cell subsets.

Lebow

Bonavida

1990

Proc. Natl. Acad. Sci. U.S.A.

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Natural killer (NK) cells form three functionally distinct populations of effectors: competent cytolytic effectors able to bind and kill target cells and two subsets of nonlytic effectors, one able and the other unable to bind target cells. A flow cytometric method was developed, based on size and two-color fluorescence of NK cell-target conjugates, for the characterization and sorting of highly purified subpopulations-killer cells, nonkiller binder cells, and free cells. Ultrastructural examination revealed that granule content was reduced in the killer cells and absent in most of the binder cells. Quantitative differences in the expression level of HLA class I, CD11b (C3bi receptor), and CD16 (receptor for the Fc portion of IgG) antigens could differentiate the subsets. The killer phenotype was HLAIO, CD11b"Y hi, and CD16VerY 10; the binder phenotype was CD11bh' and CD16IO; and the free-cell phenotype was CD11bIO and CD16M1. Cell activation was not requisite for lytic function because no difference in either expression of activation markers or cell cycle could be established among the sorted subpopulations. Although recycling function was inhibited, retention of lytic activity was enriched 4-fold in the sorted killer cell population. These results represent characterization of a successful bulk isolation of competent killer, nonkiller binder, and free cells in human NK-cell populations and should aid our understanding of NK-cell development, lineage, and function.

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Section: Determination Of Nk Binder-and Killer-cell Frequencies Bymentioning

confidence: 99%

Purification and characterization of cytolytic and noncytolytic human natural killer cell subsets.

Lebow

Bonavida

1990

Proc. Natl. Acad. Sci. U.S.A.

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“…There is no evidence that levamisole can augment NK cells [24]. In vitro investiga tions in our study on RCC patients showed that levami sole and OMPI are apparently without any influence on NK cell activity.…”

Section: Discussionmentioning

confidence: 65%

Natural Killer Cells in Patients with Renal Cell Cancer

Hofmann¹,

Lehmer²,

Reidel

et al. 1985

Urol Int

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Cytotoxic activity of natural killer (NK) cells in peripheral blood was studied in 60 patients with renal cell carcinoma (RCC) by means of a ⁵¹Cr release assay against K 562 cells, homologous and autologous RCC. All patients were tested prior to surgery and thereafter followed up for 1 year. Immunostimulating agents as interferon, levamisole, OMPI, and various bacterial extracts were tested for their boosting capacity on NK cells. Patients with localized tumor showed increased activity in comparison to age-matched controls, whereas NK cell activity and interferon-boosted activity in patients with advanced disease and tumor spread was decreased. Augmentation of NK activity could only be achieved by interferon, while application of levamisole, OMPI, and bacterial extracts resulted rather in suppression of cytotoxic activity.

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“…Preparation ofperipheral blood lymphocytes (PBL) Human peripheral blood mononuclear cells were isolated from defibrinated or heparinized blood as previously described (Shau & Dawson, 1982). Adherent cells were depleted by incubating the mononuclear preparations (4 x 106 cells ml-1) at 370 for 1 h on plastic tissue culture flasks and by passing cell suspensions through nylon wool columns.…”

Section: Methodsmentioning

confidence: 99%

“…Percollfractionation of PBL NK effector cells were enriched by centrifugation on discontinuous density gradients of Percoll Timonen & Saksela, 1981 (Shau & Dawson, 1982).…”

Section: Methodsmentioning

confidence: 99%