Human natural killing against ovarian carcinoma

Shau, Hungyi; Koren, Hillel S.; Dawson, Jeffrey R.

doi:10.1038/bjc.1983.107

Cited by 8 publications

(2 citation statements)

References 19 publications

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“…Cultured cell lines derived from the same tumour may differ among each other and differ from their precursor cells in the original tumour. For example, it is known that cell culture may produce alterations in chromosome number and may lead to increased sensitivity to lysis by natural killer (NK) cells present in human peripheral blood (Shau et al, 1983). In contrast, the cells of the original tumour when freshly isolated are usually resistant to lysis (Shau et al, 1983;Vanky et al, 1984).…”

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confidence: 99%

“…For example, it is known that cell culture may produce alterations in chromosome number and may lead to increased sensitivity to lysis by natural killer (NK) cells present in human peripheral blood (Shau et al, 1983). In contrast, the cells of the original tumour when freshly isolated are usually resistant to lysis (Shau et al, 1983;Vanky et al, 1984). Alterations in susceptibility to lysis are likely due to either culture-induced changes in cell phenotype or to selection of a minor subpopulation whose killing by NK cells is inapparent when diluted by a large number of NK-resistant cells.…”

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Human ovarian carcinoma: evidence for patient-related differences in susceptibility to cytotoxic effectors that attack different cellular subpopulations within a tumour

Clark

Hirte²,

Buick³

1988

Br J Cancer

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Summary Human ovarian carcinoma cells obtained from ascites were tested for susceptibility to lysis by peripheral blood NK cells, alpha-interferon-activated NK cells, and interleukin 2-activated killer cells. Cryopreserved tumour cell preparations were used to allow repeated testing of the same target, and the tumour cells were fractionated using albumin density gradients to determine if fractions containing clonogenic (stem) cells were killed. Four tumour cell donors were studied and each showed a different pattern of susceptibility of unfractionated tumour to lysis by different effector cells. Using fractionated tumour cells, we found that NK and interferon-activated NK cells did not always lyse cells in the clonogenic fractions and that interferon activation could in some cases shift killing away from the clonogenic fractions and towards the peak of proliferating (but not self-renewing) colony forming cells. Interleukin 2-activated killer cells (LAK) however, killed the fractions containing clonogenic cells in all 4 cases. The magnitude of killing seen when fractions of the original tumour were tested was often striking when compared to lysis of the unfractionated cells. Apparent heterogeneity between patients and stem cell susceptibility to effector cells may be important determinants of the efficacy of treatment of patients with biologic response modifiers such as interferon and interleukin 2.

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confidence: 99%

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confidence: 99%

Human ovarian carcinoma: evidence for patient-related differences in susceptibility to cytotoxic effectors that attack different cellular subpopulations within a tumour

Clark

Hirte²,

Buick³

1988

Br J Cancer

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Adenovirus Inversely Modulates Target Cell Class I Antigen Expression and Sensitivity to Natural Killing

Storkus

Cresswell

Patterson

et al. 1989

Immunobiology of HLA

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Studies on cytotoxicity generated in human mixed lymphocyte culture

Shau¹,

Gray²,

Golub³

1988

Cancer Immunol Immunother

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High levels of cytotoxic activity against the natural killer (NK) cell-sensitive target K562 and the NK-resistant target UCLA-SO-M14 (M14) can be generated in vitro either by mixed lymphocyte culture (MLC) or by culture of lymphocytes in interleukin 2 (IL2) (lymphokine activated killer (LAK) cells). The purpose of this study was to identify similarities and differences between MLC-LAK and IL2-LAK cells and allospecific cytotoxic T cells. Induction of cytotoxicity against K562 and M14 in both culture systems was inhibited by antibodies specific either for IL2 or the Tac IL2 receptor. Like NK effector cells, the precursors for the MLC-LAK cells were low density large lymphocytes. However these precursors differed from the large granular lymphocytes that mediated NK cytolysis in sensitivity to the toxic lysosomotropic agent L-leucine methyl ester (LME). The resistance of the MLC-LAK precursors to LME indicated that the precursors included large agranular lymphocytes. Although interferon-gamma (IFN-gamma) is produced in MLC and in IL2 containing cultures, it is not required for induction of either type of cytotoxic activity. Neutralization of IFN-gamma in MLC- and IL2-containing cultures with specific antibodies had no effect on the induction of cytotoxic activities. Both allospecific cytotoxic T lymphocyte (CTL) and LAK activities were enhanced by IL2 and IFN-gamma at the effector cell stage. However, the mechanism of cytolysis was different in the two systems. NK- and MLC-induced LAK activities were independent of CD3-T cell receptor complex while CTL activity was blocked by monoclonal antibodies specific for the CD3 antigen. These results suggest that NK and the in vitro induced LAK cytotoxicities are a family of related functions that differ from CTL. Furthermore, MLC-induced and IL2-induced cytotoxicities against K562 and M14 appear to be identical.

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Human natural killing against ovarian carcinoma

Cited by 8 publications

References 19 publications

Human ovarian carcinoma: evidence for patient-related differences in susceptibility to cytotoxic effectors that attack different cellular subpopulations within a tumour

Human ovarian carcinoma: evidence for patient-related differences in susceptibility to cytotoxic effectors that attack different cellular subpopulations within a tumour

Adenovirus Inversely Modulates Target Cell Class I Antigen Expression and Sensitivity to Natural Killing

Studies on cytotoxicity generated in human mixed lymphocyte culture

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